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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06400 and PS417_06405 are separated by 26 nucleotides
PS417_06405 and PS417_06410 overlap by 4 nucleotides
PS417_06410 and PS417_06415 are separated by 57 nucleotides
PS417_06415 and PS417_06420 are separated by 240 nucleotides
PS417_06400: PS417_06400 - succinylglutamate desuccinylase, at 1,418,866 to 1,419,984
_06400
PS417_06405: PS417_06405 - amino acid ABC transporter permease, at 1,420,011 to 1,420,721
_06405
PS417_06410: PS417_06410 - amino acid ABC transporter permease, at 1,420,718 to 1,421,446
_06410
PS417_06415: PS417_06415 - ABC transporter substrate-binding protein, at 1,421,504 to 1,422,283
_06415
PS417_06420: PS417_06420 - ATP-dependent DNA ligase, at 1,422,524 to 1,424,158
_06420
Group
Condition
PS417
_06400
PS417
_06405
PS417
_06410
PS417
_06415
PS417
_06420
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
-1.0
-0.8
-0.7
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
-0.9
-0.5
-0.7
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.0
-0.9
-0.8
-0.9
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
-0.9
+0.0
-0.2
+0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.3
-0.3
-0.5
-0.5
+0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.7
-0.2
-0.3
-0.4
+0.1
soil
soil sample 5; outgrowth in LB
-1.6
-0.3
+0.2
+0.3
+0.1
phage
JP1 MOI 1
-1.1
-0.1
-0.3
+0.2
+0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.3
-0.6
-0.4
+0.2
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.3
+0.3
-0.6
-0.4
supernatant control
Vogels_fungal_media 0.1X
-0.7
+0.0
-0.4
-0.3
+0.4
motility
inner cut, LB soft agar motility assay
-0.6
-0.2
+0.2
-0.5
+0.3
phage
P. simiae ORA MOI 1
-1.0
-0.1
+0.1
+0.4
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.0
-0.3
-0.2
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.1
+0.2
-0.5
-0.4
+0.3
phage
P. simiae Grant Run MOI 10
+1.0
-0.6
-0.6
+0.2
-0.5
seeds
Growth on radish seeds for 72 hours
+0.5
-0.6
-0.2
-0.5
+0.5
carbon source
2'-Deoxyinosine 5 mM (C)
+0.4
-0.4
+0.2
-0.3
-0.3
nophagecontrol
Only library
-0.9
+0.0
-0.1
+0.6
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
-0.0
-0.2
-0.7
+0.0
seeds
Growth on radish seeds for 72 hours
-0.2
-0.3
-0.3
+0.2
+0.5
liquid stress
80% Methanol 3 mM; liquid stress
+0.3
+0.3
-0.4
+0.2
-0.3
phage
P. simiae Grant Run MOI 0.1
+0.8
+0.2
-0.2
+0.1
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
+0.2
-0.2
-0.1
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.9
+0.2
+0.1
-0.5
+0.1
stress
Doxycycline hyclate 0.001 mg/ml
+0.9
-0.0
-0.1
-0.3
+0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+0.5
+1.1
-0.5
-0.4
phage
P. simiae ORA MOI 0.1
+0.5
-0.1
+0.5
-0.2
+0.3
agar plate interaction control
Taped volatile agar plate with no fungus
+0.8
+0.3
+0.4
+0.1
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+1.0
+0.3
+0.2
+0.2
+0.3
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