Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06245 and PS417_06250 are separated by 74 nucleotidesPS417_06250 and PS417_06255 are separated by 45 nucleotidesPS417_06255 and PS417_06260 are separated by 3 nucleotidesPS417_06260 and PS417_06265 are separated by 111 nucleotides PS417_06245: PS417_06245 - zinc metallopeptidase RseP, at 1,384,901 to 1,386,253 _06245 PS417_06250: PS417_06250 - membrane protein, at 1,386,328 to 1,388,715 _06250 PS417_06255: PS417_06255 - Skp, at 1,388,761 to 1,389,264 _06255 PS417_06260: PS417_06260 - UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase, at 1,389,268 to 1,390,323 _06260 PS417_06265: PS417_06265 - 3-hydroxyacyl-ACP dehydratase, at 1,390,435 to 1,390,875 _06265
Group Condition PS417_06245 PS417_06250 PS417_06255 PS417_06260 PS417_06265
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -5.5 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -5.4 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -5.4 N.D. N.D. N.D. N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -4.2 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -4.0 N.D. N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.1X -4.0 N.D. N.D. N.D. N.D.
stress Phosphomycin 0.003 mg/ml -3.9 N.D. N.D. N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus -3.7 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.6 N.D. N.D. N.D. N.D.
stress Polymyxin B sulfate 0.006 mg/ml -3.3 N.D. N.D. N.D. N.D.
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 -3.2 N.D. N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -3.2 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -3.2 N.D. N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -3.1 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -3.1 N.D. N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -3.1 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.1 N.D. N.D. N.D. N.D.
phage P. simiae ORA MOI 0.1 -3.1 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -3.1 N.D. N.D. N.D. N.D.
stress Cisplatin 0.01 mg/ml -3.0 N.D. N.D. N.D. N.D.
soil soil sample 2; outgrowth in LB -3.0 N.D. N.D. N.D. N.D.
phage P. simiae Grant Run MOI 10 -2.9 N.D. N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 10 -2.8 N.D. N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 0.1 -2.8 N.D. N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.8 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.7 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.9 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.9 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +3.0 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +3.3 N.D. N.D. N.D. N.D.
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