Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06085 and PS417_06090 are separated by 27 nucleotidesPS417_06090 and PS417_06095 are separated by 3 nucleotidesPS417_06095 and PS417_06100 are separated by 96 nucleotidesPS417_06100 and PS417_06105 are separated by 190 nucleotides PS417_06085: PS417_06085 - 2-methylisocitrate lyase, at 1,349,311 to 1,350,138 _06085 PS417_06090: PS417_06090 - extensin, at 1,350,166 to 1,350,840 _06090 PS417_06095: PS417_06095 - energy transducer TonB, at 1,350,844 to 1,351,464 _06095 PS417_06100: PS417_06100 - SAM-dependent methyltransferase, at 1,351,561 to 1,352,343 _06100 PS417_06105: PS417_06105 - TetR family transcriptional regulator, at 1,352,534 to 1,353,169 _06105
Group Condition PS417_06085 PS417_06090 PS417_06095 PS417_06100 PS417_06105
agar plate interaction control Taped volatile agar plate with no fungus -0.2 -0.0 -4.6 +0.5 +0.0
phage JP1 MOI 10 -0.4 +0.1 -4.0 +0.3 -0.0
phage P. simiae Grant Run MOI 0.1 -0.3 +0.0 -2.0 -0.5 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.0 -1.8 -0.1 -0.4
nophagecontrol Only library -0.4 +0.0 -3.3 +1.0 -0.1
motility inner cut, LB soft agar motility assay -0.5 -0.2 -1.5 -0.3 -0.1
phage P. simiae Grant Run MOI 0.1 -0.0 +0.2 -1.6 -0.8 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.1 -0.4 -0.7 -2.0 +0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 -0.0 -1.5 -0.7 -0.5
seeds Growth on radish seeds for 72 hours -0.7 +0.1 -0.6 -0.7 -0.4
stress Neomycin 0.04 mg/ml -0.1 +0.1 -1.2 -0.9 +0.1
stress Gentamicin 0.008 mg/ml +0.0 +0.0 +0.7 -2.6 -0.1
carbon source 2-Deoxy-D-Ribose (C) -0.2 +0.2 -0.7 -1.3 +0.2
phage Wcs_1 +0.3 -0.3 -1.9 -0.2 +0.1
root root sample 6; outgrowth in LB +0.4 +0.2 -2.2 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 +0.2 +0.6 -2.9 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.4 +0.2 -1.6 +0.1
rhizosphere rhizosphere sample 8; outgrowth in LB -0.6 +0.3 -1.5 +0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.1 +0.7 -2.8 +0.6
seeds Growth on radish seeds for 72 hours +0.5 +0.4 -0.4 -2.0 +0.5
phage JP1 MOI 10 -0.9 -0.3 -0.7 +0.9 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.1 +1.2 -0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.0 +0.2 +1.3 -1.1 +0.0
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 +0.7 -1.1 +0.7 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -0.3 +0.7 +0.9 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 -0.3 +0.4 +1.6 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.1 +1.6 +0.5 -0.2
stress methylglyoxal 0.032 vol% -0.3 +0.1 +0.2 +1.1 +1.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 +0.3 +1.5 +0.9 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.1 +0.1 +3.0 -0.1
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