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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06080 and PS417_06085 are separated by 27 nucleotides
PS417_06085 and PS417_06090 are separated by 27 nucleotides
PS417_06090 and PS417_06095 are separated by 3 nucleotides
PS417_06095 and PS417_06100 are separated by 96 nucleotides
PS417_06080: PS417_06080 - hypothetical protein, at 1,348,423 to 1,349,283
_06080
PS417_06085: PS417_06085 - 2-methylisocitrate lyase, at 1,349,311 to 1,350,138
_06085
PS417_06090: PS417_06090 - extensin, at 1,350,166 to 1,350,840
_06090
PS417_06095: PS417_06095 - energy transducer TonB, at 1,350,844 to 1,351,464
_06095
PS417_06100: PS417_06100 - SAM-dependent methyltransferase, at 1,351,561 to 1,352,343
_06100
Group
Condition
PS417
_06080
PS417
_06085
PS417
_06090
PS417
_06095
PS417
_06100
agar plate interaction control
Taped volatile agar plate with no fungus
+0.0
-0.2
-0.0
-4.6
+0.5
phage
JP1 MOI 10
-0.3
-0.4
+0.1
-4.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.2
+0.1
-0.4
-0.7
-2.0
phage
P. simiae Grant Run MOI 0.1
+0.0
-0.3
+0.0
-2.0
-0.5
nophagecontrol
Only library
-0.1
-0.4
+0.0
-3.3
+1.0
motility
inner cut, LB soft agar motility assay
-0.2
-0.5
-0.2
-1.5
-0.3
stress
Gentamicin 0.008 mg/ml
-0.7
+0.0
+0.0
+0.7
-2.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.2
-0.3
+0.2
+0.6
-2.9
phage
P. simiae ORA MOI 10
-0.2
+0.0
-0.2
-1.2
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.5
+0.0
-1.8
-0.1
carbon source
2-Deoxy-D-Ribose (C)
-0.1
-0.2
+0.2
-0.7
-1.3
phage
Wcs_1
+0.0
+0.3
-0.3
-1.9
-0.2
phage
P. simiae Grant Run MOI 0.1
+0.2
-0.0
+0.2
-1.6
-0.8
phage
P. simiae ORA MOI 0.1
-0.5
-0.4
-0.2
-1.0
+0.3
root
root sample 6; outgrowth in LB
+0.2
+0.4
+0.2
-2.2
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
+0.1
-0.1
+0.7
-2.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
-0.2
-0.2
-1.5
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
+0.1
-0.4
+0.2
-1.6
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.3
+0.3
-0.0
-1.5
-0.7
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.3
-0.6
+0.3
-1.5
+0.2
seeds
Growth on radish seeds for 72 hours
+0.3
+0.5
+0.4
-0.4
-2.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.4
-0.0
-1.4
+0.3
phage
JP1 MOI 10
-0.2
-0.9
-0.3
-0.7
+0.9
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.1
-0.0
+0.7
-1.1
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.6
-0.1
+1.2
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
+0.2
+0.0
+0.2
+1.3
-1.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.4
-0.3
+0.4
+1.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.2
+0.1
+1.6
+0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.1
-0.5
+0.3
+1.5
+0.9
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
+0.2
-0.1
+0.1
+3.0
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