Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06080 and PS417_06085 are separated by 27 nucleotidesPS417_06085 and PS417_06090 are separated by 27 nucleotidesPS417_06090 and PS417_06095 are separated by 3 nucleotidesPS417_06095 and PS417_06100 are separated by 96 nucleotides PS417_06080: PS417_06080 - hypothetical protein, at 1,348,423 to 1,349,283 _06080 PS417_06085: PS417_06085 - 2-methylisocitrate lyase, at 1,349,311 to 1,350,138 _06085 PS417_06090: PS417_06090 - extensin, at 1,350,166 to 1,350,840 _06090 PS417_06095: PS417_06095 - energy transducer TonB, at 1,350,844 to 1,351,464 _06095 PS417_06100: PS417_06100 - SAM-dependent methyltransferase, at 1,351,561 to 1,352,343 _06100
Group Condition PS417_06080 PS417_06085 PS417_06090 PS417_06095 PS417_06100
agar plate interaction control Taped volatile agar plate with no fungus +0.0 -0.2 -0.0 -4.6 +0.5
phage JP1 MOI 10 -0.3 -0.4 +0.1 -4.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +0.1 -0.4 -0.7 -2.0
phage P. simiae Grant Run MOI 0.1 +0.0 -0.3 +0.0 -2.0 -0.5
nophagecontrol Only library -0.1 -0.4 +0.0 -3.3 +1.0
motility inner cut, LB soft agar motility assay -0.2 -0.5 -0.2 -1.5 -0.3
stress Gentamicin 0.008 mg/ml -0.7 +0.0 +0.0 +0.7 -2.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 -0.3 +0.2 +0.6 -2.9
phage P. simiae ORA MOI 10 -0.2 +0.0 -0.2 -1.2 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.5 +0.0 -1.8 -0.1
carbon source 2-Deoxy-D-Ribose (C) -0.1 -0.2 +0.2 -0.7 -1.3
phage Wcs_1 +0.0 +0.3 -0.3 -1.9 -0.2
phage P. simiae Grant Run MOI 0.1 +0.2 -0.0 +0.2 -1.6 -0.8
phage P. simiae ORA MOI 0.1 -0.5 -0.4 -0.2 -1.0 +0.3
root root sample 6; outgrowth in LB +0.2 +0.4 +0.2 -2.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.1 -0.1 +0.7 -2.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 -0.2 -0.2 -1.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 +0.1 -0.4 +0.2 -1.6
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 +0.3 -0.0 -1.5 -0.7
rhizosphere rhizosphere sample 8; outgrowth in LB +0.3 -0.6 +0.3 -1.5 +0.2
seeds Growth on radish seeds for 72 hours +0.3 +0.5 +0.4 -0.4 -2.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.4 -0.0 -1.4 +0.3
phage JP1 MOI 10 -0.2 -0.9 -0.3 -0.7 +0.9
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 -0.0 +0.7 -1.1 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.6 -0.1 +1.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.0 +0.2 +1.3 -1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.4 -0.3 +0.4 +1.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.2 +0.1 +1.6 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.5 +0.3 +1.5 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 +0.2 -0.1 +0.1 +3.0
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