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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_06070 and PS417_06075 are separated by 160 nucleotides
PS417_06075 and PS417_06080 overlap by 1 nucleotides
PS417_06080 and PS417_06085 are separated by 27 nucleotides
PS417_06085 and PS417_06090 are separated by 27 nucleotides
PS417_06070: PS417_06070 - peptidase M22, at 1,347,286 to 1,347,972
_06070
PS417_06075: PS417_06075 - hypothetical protein, at 1,348,133 to 1,348,423
_06075
PS417_06080: PS417_06080 - hypothetical protein, at 1,348,423 to 1,349,283
_06080
PS417_06085: PS417_06085 - 2-methylisocitrate lyase, at 1,349,311 to 1,350,138
_06085
PS417_06090: PS417_06090 - extensin, at 1,350,166 to 1,350,840
_06090
Group
Condition
PS417
_06070
PS417
_06075
PS417
_06080
PS417
_06085
PS417
_06090
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.5
-0.1
-0.7
-0.2
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
-3.2
-0.1
+0.2
+0.2
phage
P. simiae Grant Run MOI 0.1
N.D.
-2.1
+0.0
-0.3
+0.0
soil
soil sample 6; outgrowth in LB
N.D.
-2.0
+0.2
-0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.6
-0.0
-0.3
-0.2
soil
soil sample 3; outgrowth in LB
N.D.
-2.2
+0.2
+0.2
-0.1
motility
inner cut, LB soft agar motility assay
N.D.
-0.9
-0.2
-0.5
-0.2
stress
Gentamicin 0.008 mg/ml
N.D.
-1.0
-0.7
+0.0
+0.0
carbon source
Gly-Glu (C)
N.D.
-1.3
-0.1
-0.2
-0.1
phage
JP1 MOI 10
N.D.
-0.3
-0.2
-0.9
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.6
+0.1
+0.1
-0.2
carbon source
Carbon source D-Mannitol 2.5 mM
N.D.
-0.9
+0.1
-0.5
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.9
+0.2
-0.2
-0.2
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.1
+0.1
-0.2
+0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
-1.0
+0.1
-0.3
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.9
+0.1
-0.3
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.8
+0.1
-0.5
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.7
+0.2
-0.5
+0.2
seeds
Growth on radish seeds for 72 hours
N.D.
-1.9
+0.3
+0.5
+0.4
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
-0.5
+0.3
-0.6
+0.3
seeds
Growth on radish seeds for 72 hours
N.D.
-1.1
+0.6
+0.1
+0.1
phage
P. simiae ORA MOI 0.1
N.D.
+1.2
-0.5
-0.4
-0.2
phage
JP1 MOI 10
N.D.
+0.8
-0.3
-0.4
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.9
+0.4
-0.6
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
+1.0
+0.3
-0.1
-0.2
carbon source
L-Valine (C)
N.D.
+1.6
+0.2
-0.5
-0.1
stress
methylglyoxal 0.032 vol%
N.D.
+1.7
-0.2
-0.3
+0.1
root
root sample 6; outgrowth in LB
N.D.
+0.7
+0.2
+0.4
+0.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
+1.8
+0.1
+0.0
-0.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
+2.7
+0.3
+0.3
-0.0
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