Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_06070 and PS417_06075 are separated by 160 nucleotidesPS417_06075 and PS417_06080 overlap by 1 nucleotidesPS417_06080 and PS417_06085 are separated by 27 nucleotidesPS417_06085 and PS417_06090 are separated by 27 nucleotides PS417_06070: PS417_06070 - peptidase M22, at 1,347,286 to 1,347,972 _06070 PS417_06075: PS417_06075 - hypothetical protein, at 1,348,133 to 1,348,423 _06075 PS417_06080: PS417_06080 - hypothetical protein, at 1,348,423 to 1,349,283 _06080 PS417_06085: PS417_06085 - 2-methylisocitrate lyase, at 1,349,311 to 1,350,138 _06085 PS417_06090: PS417_06090 - extensin, at 1,350,166 to 1,350,840 _06090
Group Condition PS417_06070 PS417_06075 PS417_06080 PS417_06085 PS417_06090
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.5 -0.1 -0.7 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -3.2 -0.1 +0.2 +0.2
phage P. simiae Grant Run MOI 0.1 N.D. -2.1 +0.0 -0.3 +0.0
soil soil sample 6; outgrowth in LB N.D. -2.0 +0.2 -0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.6 -0.0 -0.3 -0.2
soil soil sample 3; outgrowth in LB N.D. -2.2 +0.2 +0.2 -0.1
motility inner cut, LB soft agar motility assay N.D. -0.9 -0.2 -0.5 -0.2
stress Gentamicin 0.008 mg/ml N.D. -1.0 -0.7 +0.0 +0.0
carbon source Gly-Glu (C) N.D. -1.3 -0.1 -0.2 -0.1
phage JP1 MOI 10 N.D. -0.3 -0.2 -0.9 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.6 +0.1 +0.1 -0.2
carbon source Carbon source D-Mannitol 2.5 mM N.D. -0.9 +0.1 -0.5 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.9 +0.2 -0.2 -0.2
solid stress Fraxetin 3 mM; solid stress N.D. -1.1 +0.1 -0.2 +0.1
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -1.0 +0.1 -0.3 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.9 +0.1 -0.3 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.8 +0.1 -0.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 +0.2 -0.5 +0.2
seeds Growth on radish seeds for 72 hours N.D. -1.9 +0.3 +0.5 +0.4
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.5 +0.3 -0.6 +0.3
seeds Growth on radish seeds for 72 hours N.D. -1.1 +0.6 +0.1 +0.1
phage P. simiae ORA MOI 0.1 N.D. +1.2 -0.5 -0.4 -0.2
phage JP1 MOI 10 N.D. +0.8 -0.3 -0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.9 +0.4 -0.6 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +1.0 +0.3 -0.1 -0.2
carbon source L-Valine (C) N.D. +1.6 +0.2 -0.5 -0.1
stress methylglyoxal 0.032 vol% N.D. +1.7 -0.2 -0.3 +0.1
root root sample 6; outgrowth in LB N.D. +0.7 +0.2 +0.4 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +1.8 +0.1 +0.0 -0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +2.7 +0.3 +0.3 -0.0
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