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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_05985 and PS417_05990 are separated by 51 nucleotides
PS417_05990 and PS417_06000 are separated by 453 nucleotides
PS417_06000 and PS417_06005 are separated by 83 nucleotides
PS417_06005 and PS417_06010 are separated by 106 nucleotides
PS417_05985: PS417_05985 - chemotaxis protein CheY, at 1,331,450 to 1,332,460
_05985
PS417_05990: PS417_05990 - diguanylate cyclase, at 1,332,512 to 1,333,513
_05990
PS417_06000: PS417_06000 - peptide chain release factor 2, at 1,333,967 to 1,334,848
_06000
PS417_06005: PS417_06005 - lysyl-tRNA synthetase, at 1,334,932 to 1,336,431
_06005
PS417_06010: PS417_06010 - TetR family transcriptional regulator, at 1,336,538 to 1,337,254
_06010
Group
Condition
PS417
_05985
PS417
_05990
PS417
_06000
PS417
_06005
PS417
_06010
phage
JP1 MOI 1
-3.7
-0.1
N.D.
N.D.
-0.3
phage
JP1 MOI 10
-3.1
+0.6
N.D.
N.D.
-0.7
phage
JP1 MOI 0.1
-3.4
+0.5
N.D.
N.D.
-0.3
phage
P. simiae Grant Run MOI 0.1
-3.6
+0.6
N.D.
N.D.
-0.2
phage
P. simiae OR Antrim MOI 1
-3.0
+0.3
N.D.
N.D.
-0.1
phage
P. simiae OR Antrim MOI 10
-2.8
+0.0
N.D.
N.D.
+0.2
phage
P. simiae OR Antrim MOI 1
-3.1
+0.7
N.D.
N.D.
-0.3
phage
JP1 MOI 10
-2.9
+1.0
N.D.
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-3.0
+0.6
N.D.
N.D.
+0.0
phage
P. simiae Grant Run MOI 10
-3.0
+0.8
N.D.
N.D.
-0.1
phage
P. simiae ORA MOI 1
-2.7
+0.5
N.D.
N.D.
-0.0
phage
P. simiae ORA MOI 10
-2.4
+0.4
N.D.
N.D.
-0.1
phage
P. simiae OR Antrim MOI 0.1
-2.9
+0.8
N.D.
N.D.
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.6
+0.6
N.D.
N.D.
-0.1
phage
P. simiae Grant Run MOI 0.1
-2.6
+0.4
N.D.
N.D.
+0.3
stress
Choline chloride 300 mM
-2.3
+0.6
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.0
+0.8
N.D.
N.D.
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.5
+1.0
N.D.
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.0
+0.8
N.D.
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.8
+1.4
N.D.
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
+1.3
N.D.
N.D.
+0.3
soil
soil sample 5; outgrowth in LB
+2.3
-0.8
N.D.
N.D.
+0.3
ecofab
LB in EcoFAB_3.5mL
+2.8
-0.0
N.D.
N.D.
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+2.9
-0.1
N.D.
N.D.
+0.2
rhizosphere
rhizosphere sample 1; outgrowth in LB
+2.6
+0.2
N.D.
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+3.2
-0.0
N.D.
N.D.
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+3.4
-0.1
N.D.
N.D.
+0.2
rhizosphere
rhizosphere sample 2; outgrowth in LB
+3.5
+0.1
N.D.
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+3.3
+0.3
N.D.
N.D.
+0.2
root
root sample 6; outgrowth in LB
+4.3
+0.4
N.D.
N.D.
+0.1
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