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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_05470 and PS417_05475 are separated by 82 nucleotides
PS417_05475 and PS417_05480 overlap by 4 nucleotides
PS417_05480 and PS417_05485 overlap by 4 nucleotides
PS417_05485 and PS417_05490 are separated by 80 nucleotides
PS417_05470: PS417_05470 - DSBA oxidoreductase, at 1,232,812 to 1,233,465
_05470
PS417_05475: PS417_05475 - arginase, at 1,233,548 to 1,234,468
_05475
PS417_05480: PS417_05480 - transporter, at 1,234,465 to 1,234,845
_05480
PS417_05485: PS417_05485 - transporter, at 1,234,842 to 1,235,246
_05485
PS417_05490: PS417_05490 - fatty acid desaturase, at 1,235,327 to 1,236,199
_05490
Group
Condition
PS417
_05470
PS417
_05475
PS417
_05480
PS417
_05485
PS417
_05490
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.0
-1.8
N.D.
N.D.
-0.1
supernatant control
Vogels_fungal_media 0.1X
-0.6
-1.2
N.D.
N.D.
-0.1
carbon source
2'-Deoxyinosine 5 mM (C)
-0.4
-1.2
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.2
-0.5
N.D.
N.D.
+0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.8
-1.1
N.D.
N.D.
+0.2
soil
soil sample 3; outgrowth in LB
-0.3
-1.5
N.D.
N.D.
+0.2
phage
P. simiae ORA MOI 0.1
-1.0
-0.5
N.D.
N.D.
+0.1
soil
soil sample 6; outgrowth in LB
+0.5
-1.7
N.D.
N.D.
+0.0
soil
soil sample 7; outgrowth in LB
-0.5
-0.9
N.D.
N.D.
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
-1.2
N.D.
N.D.
-0.5
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
+0.4
-0.7
N.D.
N.D.
-0.4
carbon source
L-Valine (C)
-1.1
+0.5
N.D.
N.D.
+0.0
phage
P. simiae Grant Run MOI 0.1
+0.6
-0.9
N.D.
N.D.
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.7
-0.7
N.D.
N.D.
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.2
+1.2
N.D.
N.D.
-0.1
seeds
Growth on radish seeds for 72 hours
+0.4
+1.0
N.D.
N.D.
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+1.2
N.D.
N.D.
+0.2
stress
Neomycin 0.04 mg/ml
-0.2
+0.5
N.D.
N.D.
+0.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+1.4
N.D.
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.9
+2.1
N.D.
N.D.
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+1.6
N.D.
N.D.
+0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+1.5
N.D.
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.5
+1.1
N.D.
N.D.
+0.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.4
+1.4
N.D.
N.D.
+0.1
stress
Gentamicin 0.008 mg/ml
+0.5
+1.1
N.D.
N.D.
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
+1.6
N.D.
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
+1.7
N.D.
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.6
+1.4
N.D.
N.D.
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.5
+1.7
N.D.
N.D.
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.8
+2.2
N.D.
N.D.
+0.7
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