Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_00535 and PS417_00540 are separated by 80 nucleotidesPS417_00540 and PS417_00545 are separated by 136 nucleotidesPS417_00545 and PS417_00550 are separated by 263 nucleotidesPS417_00550 and PS417_00555 are separated by 317 nucleotides PS417_00535: PS417_00535 - Holliday junction resolvase, at 108,229 to 108,537 _00535 PS417_00540: PS417_00540 - hypothetical protein, at 108,618 to 108,938 _00540 PS417_00545: PS417_00545 - hypothetical protein, at 109,075 to 109,260 _00545 PS417_00550: PS417_00550 - hypothetical protein, at 109,524 to 109,739 _00550 PS417_00555: PS417_00555 - acetyl-CoA hydrolase, at 110,057 to 111,550 _00555
Group Condition PS417_00535 PS417_00540 PS417_00545 PS417_00550 PS417_00555
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. -0.9 -5.1 +0.1
soil soil sample 3; outgrowth in LB -1.7 N.D. -0.7 -1.5 +0.5
phage P. simiae Grant Run MOI 0.1 -0.5 N.D. +0.1 -3.4 +0.5
stress Cisplatin 0.01 mg/ml -1.1 N.D. -0.2 -1.6 -0.0
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -0.7 N.D. -0.3 -1.7 +0.0
phage P. simiae ORA MOI 10 -1.7 N.D. -0.8 -0.4 +0.2
motility inner cut, LB soft agar motility assay -1.3 N.D. -0.3 -1.2 +0.3
carbon source RCH2_defined_glucose with MS_media 1x -0.5 N.D. -0.1 -1.9 -0.2
root root sample 6; outgrowth in LB -0.1 N.D. +1.0 -3.6 +0.1
seeds Growth on radish seeds for 72 hours +0.3 N.D. -0.4 -2.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.9 N.D. -1.0 -0.8 +0.2
motility outer cut, LB soft agar motility assay -1.5 N.D. -0.2 -1.1 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.5 N.D. -0.5 +0.7 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.8 N.D. -0.9 +0.8 -0.4
soil soil sample 6; outgrowth in LB -2.6 N.D. -0.2 +0.6 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.9 N.D. -0.5 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.1 N.D. +0.4 -0.1 +0.2
carbon source 2'-Deoxyinosine 5 mM (C) +0.9 N.D. -0.3 -1.8 -0.0
phage P. simiae ORA MOI 0.1 -1.1 N.D. -0.7 +0.5 +0.4
supernatant control Vogels_fungal_media 0.1X -1.3 N.D. -0.4 +0.8 +0.3
phage P. simiae OR1 MOI 0.1 +0.7 N.D. +0.5 -1.9 +0.1
motility inner cut, LB soft agar motility assay -1.6 N.D. +0.2 +0.8 +0.2
motility outer cut, LB soft agar motility assay -1.6 N.D. +0.2 +0.8 +0.2
stress Gentamicin 0.008 mg/ml -1.1 N.D. -0.5 +1.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. +0.7 +0.9 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.2 N.D. +0.3 +1.0 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. -0.4 +1.6 +0.6
seeds Growth on radish seeds for 72 hours +0.7 N.D. -0.3 +2.1 -0.3
soil soil sample 2; outgrowth in LB -0.1 N.D. +1.1 +1.2 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 N.D. +0.2 +2.3 +0.2
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