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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_05330 and PS417_05335 overlap by 4 nucleotides
PS417_05335 and PS417_05340 are separated by 21 nucleotides
PS417_05340 and PS417_05345 are separated by 10 nucleotides
PS417_05345 and PS417_05350 are separated by 13 nucleotides
PS417_05330: PS417_05330 - fimbrial assembly protein, at 1,183,717 to 1,186,242
_05330
PS417_05335: PS417_05335 - molecular chaperone, at 1,186,239 to 1,187,000
_05335
PS417_05340: PS417_05340 - fimbrial protein, at 1,187,022 to 1,187,594
_05340
PS417_05345: PS417_05345 - exotoxin, at 1,187,605 to 1,188,099
_05345
PS417_05350: PS417_05350 - pilus assembly protein, at 1,188,113 to 1,188,592
_05350
Group
Condition
PS417
_05330
PS417
_05335
PS417
_05340
PS417
_05345
PS417
_05350
phage
P. simiae OR1 MOI 0.1
-0.4
-0.5
N.D.
-1.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
+0.0
N.D.
-1.0
N.D.
phage
P. simiae OR Antrim MOI 0.1
-0.1
-0.2
N.D.
-0.8
N.D.
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.1
-0.1
N.D.
-0.8
N.D.
stress
methylglyoxal 0.032 vol%
-0.1
-0.4
N.D.
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.0
-0.1
N.D.
-0.8
N.D.
stress
Tetracycline 0.001 mg/ml
-0.3
-0.3
N.D.
-0.4
N.D.
stress
Doxycycline hyclate 0.001 mg/ml
-0.5
-0.2
N.D.
-0.3
N.D.
solid stress
80% Methanol 3 mM; solid stress
+0.0
-0.2
N.D.
-0.7
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.3
N.D.
-0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.1
N.D.
-1.0
N.D.
phage
JP1 MOI 10
-0.1
+0.5
N.D.
-1.0
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
+0.3
N.D.
-1.1
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.5
N.D.
-0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.5
N.D.
-0.6
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-0.4
N.D.
+0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.3
N.D.
-0.5
N.D.
motility
outer cut, LB soft agar motility assay
-0.2
-0.2
N.D.
+0.6
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.0
-0.5
N.D.
+0.9
N.D.
soil
soil sample 3; outgrowth in LB
-0.0
-0.4
N.D.
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.3
N.D.
-0.2
N.D.
solid stress
80% Methanol 2 mM; solid stress
+0.1
-0.1
N.D.
+0.8
N.D.
phage
P. simiae ORA MOI 0.1
-0.2
+0.1
N.D.
+0.8
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
-0.3
N.D.
+1.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
+0.3
N.D.
+0.6
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
+0.3
N.D.
+0.5
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.1
+0.0
N.D.
+0.8
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.0
+0.4
N.D.
+0.6
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
+0.4
N.D.
+0.7
N.D.
root
root sample 6; outgrowth in LB
+0.2
+0.8
N.D.
+0.3
N.D.
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