Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_05295 and PS417_05300 are separated by 61 nucleotidesPS417_05300 and PS417_05305 are separated by 380 nucleotidesPS417_05305 and PS417_05310 are separated by 140 nucleotidesPS417_05310 and PS417_05315 are separated by 4 nucleotides PS417_05295: PS417_05295 - DeoR faimly transcriptional regulator, at 1,173,580 to 1,175,916 _05295 PS417_05300: PS417_05300 - glucose dehydrogenase, at 1,175,978 to 1,178,395 _05300 PS417_05305: PS417_05305 - TonB-dependent receptor, at 1,178,776 to 1,180,794 _05305 PS417_05310: PS417_05310 - hypothetical protein, at 1,180,935 to 1,181,168 _05310 PS417_05315: PS417_05315 - membrane protein, at 1,181,173 to 1,181,796 _05315
Group Condition PS417_05295 PS417_05300 PS417_05305 PS417_05310 PS417_05315
rhizosphere rhizosphere sample 2; outgrowth in LB -0.1 -0.3 +0.1 -0.7 -0.4
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.0 -0.7 -0.0 -0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 -0.7 -0.1 -0.4 -0.1
soil soil sample 3; outgrowth in LB +0.1 -0.4 -0.2 -0.7 +0.2
solid stress Fraxetin 3 mM; solid stress -0.2 -0.6 +0.2 -0.3 -0.0
stress LB_noSalt with Chloride 1100 mM -0.2 -0.7 +0.0 +0.2 +0.1
soil soil sample 2; outgrowth in LB +0.2 -0.5 -0.2 -0.5 +0.4
soil soil sample 5; outgrowth in LB +0.3 -0.4 -0.1 -0.5 +0.2
motility inner cut, LB soft agar motility assay +0.2 -0.4 -0.2 -0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.7 +0.2 -0.2 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 -0.3 +0.0 -0.4 +0.2
soil soil sample 4; outgrowth in LB +0.1 -0.7 -0.2 +0.2 +0.6
phage P. simiae OR1 MOI 0.1 -0.2 -0.2 +0.2 -0.3 +0.5
seeds Growth on radish seeds for 72 hours +0.6 -0.1 +0.2 -0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.1 +0.0 +0.4 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.2 +0.2 +0.7 +0.1
seeds Growth on radish seeds for 72 hours +0.7 -0.1 +0.3 -0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 -0.0 +0.2 +0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 -0.2 +0.3 +0.9 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 +0.2 +0.2 -0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.3 +0.1 -0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.2 -0.0 +0.8 +0.1
stress Gentamicin 0.008 mg/ml +0.4 +0.2 +0.0 -0.4 +0.7
stress 1-ethyl-3-methylimidazolium acetate 35 mM +0.2 +1.1 +0.1 -0.0 +0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.1 +0.2 +0.9 +0.3
carbon source 2'-Deoxyinosine 5 mM (C) +0.6 +0.2 +0.2 +0.3 +0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.6 +0.1 -0.0 +0.6 +0.4
phage P. simiae OR Antrim MOI 1 +0.4 +0.1 +0.3 +0.7 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 +0.3 +0.5 -0.2 +0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.1 +0.2 +1.8 +0.2
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