Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_05240 and PS417_05245 are separated by 92 nucleotidesPS417_05245 and PS417_05250 are separated by 355 nucleotidesPS417_05250 and PS417_05255 are separated by 11 nucleotidesPS417_05255 and PS417_05260 are separated by 140 nucleotides PS417_05240: PS417_05240 - Lon protease, at 1,161,025 to 1,163,445 _05240 PS417_05245: PS417_05245 - GntR family transcriptional regulator, at 1,163,538 to 1,164,527 _05245 PS417_05250: PS417_05250 - hypothetical protein, at 1,164,883 to 1,166,187 _05250 PS417_05255: PS417_05255 - hypothetical protein, at 1,166,199 to 1,167,077 _05255 PS417_05260: PS417_05260 - MFS transporter, at 1,167,218 to 1,168,558 _05260
Group Condition PS417_05240 PS417_05245 PS417_05250 PS417_05255 PS417_05260
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -0.4 N.D. -0.1 -0.5 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) +0.0 N.D. -0.5 -0.2 -0.4
seeds Growth on radish seeds for 72 hours -0.4 N.D. -0.2 -0.4 +0.1
carbon source 2'-Deoxyinosine 5 mM (C) +0.2 N.D. -0.5 -0.6 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.0 N.D. +0.2 -0.7 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 N.D. +0.2 -0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -0.2 -0.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 N.D. -0.1 -0.4 +0.2
stress Cisplatin 0.01 mg/ml -1.2 N.D. +0.5 -0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 N.D. -0.1 -0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 N.D. +0.4 -0.5 -0.2
phage P. simiae Grant Run MOI 10 +0.2 N.D. -0.1 -0.6 +0.3
stress Gentamicin 0.008 mg/ml +0.1 N.D. -0.8 +0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 N.D. +0.5 -0.4 -0.4
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.3 N.D. -0.4 +0.5 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. -0.4 +0.5 +0.1
seeds Growth on radish seeds for 72 hours -0.4 N.D. +0.2 -0.3 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 N.D. +0.5 -0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. -0.2 -0.3 +0.3
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.3 N.D. +0.2 +0.5 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. -0.3 +0.4 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. +0.4 +0.4 -0.3
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x -0.1 N.D. -0.3 +0.8 +0.1
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.2 N.D. +0.0 +0.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. +0.4 +0.8 +0.1
stress methylglyoxal 0.032 vol% +0.4 N.D. -0.1 +0.6 +0.1
liquid stress Fraxetin 3 mM; liquid stress +0.9 N.D. +0.1 -0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 N.D. +0.1 +0.4 +0.3
carbon source D-Glucose-6-Phosphate (C) +2.4 N.D. -0.1 +0.2 -0.0
carbon source D-Glucose-6-Phosphate (C) +4.2 N.D. +0.1 -0.0 +0.0
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