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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_05240 and PS417_05245 are separated by 92 nucleotides
PS417_05245 and PS417_05250 are separated by 355 nucleotides
PS417_05250 and PS417_05255 are separated by 11 nucleotides
PS417_05255 and PS417_05260 are separated by 140 nucleotides
PS417_05240: PS417_05240 - Lon protease, at 1,161,025 to 1,163,445
_05240
PS417_05245: PS417_05245 - GntR family transcriptional regulator, at 1,163,538 to 1,164,527
_05245
PS417_05250: PS417_05250 - hypothetical protein, at 1,164,883 to 1,166,187
_05250
PS417_05255: PS417_05255 - hypothetical protein, at 1,166,199 to 1,167,077
_05255
PS417_05260: PS417_05260 - MFS transporter, at 1,167,218 to 1,168,558
_05260
Group
Condition
PS417
_05240
PS417
_05245
PS417
_05250
PS417
_05255
PS417
_05260
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
-0.4
N.D.
-0.1
-0.5
-0.2
carbon source
2'-Deoxyinosine 5 mM (C)
+0.0
N.D.
-0.5
-0.2
-0.4
seeds
Growth on radish seeds for 72 hours
-0.4
N.D.
-0.2
-0.4
+0.1
carbon source
2'-Deoxyinosine 5 mM (C)
+0.2
N.D.
-0.5
-0.6
-0.1
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.0
N.D.
+0.2
-0.7
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.4
N.D.
+0.2
-0.3
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
N.D.
-0.2
-0.7
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
N.D.
-0.1
-0.4
+0.2
stress
Cisplatin 0.01 mg/ml
-1.2
N.D.
+0.5
-0.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
N.D.
-0.1
-0.2
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
N.D.
+0.4
-0.5
-0.2
phage
P. simiae Grant Run MOI 10
+0.2
N.D.
-0.1
-0.6
+0.3
stress
Gentamicin 0.008 mg/ml
+0.1
N.D.
-0.8
+0.4
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
N.D.
+0.5
-0.4
-0.4
supernatant
LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
-0.3
N.D.
-0.4
+0.5
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
-0.4
+0.5
+0.1
seeds
Growth on radish seeds for 72 hours
-0.4
N.D.
+0.2
-0.3
+0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.5
N.D.
+0.5
-0.2
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
N.D.
-0.2
-0.3
+0.3
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.3
N.D.
+0.2
+0.5
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
N.D.
-0.3
+0.4
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
+0.4
+0.4
-0.3
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
-0.1
N.D.
-0.3
+0.8
+0.1
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.2
N.D.
+0.0
+0.8
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
N.D.
+0.4
+0.8
+0.1
stress
methylglyoxal 0.032 vol%
+0.4
N.D.
-0.1
+0.6
+0.1
liquid stress
Fraxetin 3 mM; liquid stress
+0.9
N.D.
+0.1
-0.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.5
N.D.
+0.1
+0.4
+0.3
carbon source
D-Glucose-6-Phosphate (C)
+2.4
N.D.
-0.1
+0.2
-0.0
carbon source
D-Glucose-6-Phosphate (C)
+4.2
N.D.
+0.1
-0.0
+0.0
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