Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_05205 and PS417_05210 are separated by 277 nucleotidesPS417_05210 and PS417_05215 overlap by 1 nucleotidesPS417_05215 and PS417_05220 are separated by 79 nucleotidesPS417_05220 and PS417_05225 overlap by 4 nucleotides PS417_05205: PS417_05205 - pyridoxine 5'-phosphate synthase, at 1,155,078 to 1,155,821 _05205 PS417_05210: PS417_05210 - copper oxidase, at 1,156,099 to 1,157,475 _05210 PS417_05215: PS417_05215 - zinc-binding protein, at 1,157,475 to 1,157,972 _05215 PS417_05220: PS417_05220 - tRNA methyltransferase, at 1,158,052 to 1,159,008 _05220 PS417_05225: PS417_05225 - tRNA methyltransferase, at 1,159,005 to 1,159,748 _05225
Group Condition PS417_05205 PS417_05210 PS417_05215 PS417_05220 PS417_05225
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -3.6 N.D. -0.3 -0.5
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. -2.1 N.D. -0.7 -0.5
solid stress Fraxetin 3 mM; solid stress N.D. -1.8 N.D. -0.3 -0.3
solid stress Fraxetin 3 mM; solid stress N.D. -1.3 N.D. -0.6 -0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. -1.4 N.D. -0.6 -0.3
liquid stress Fraxetin 3 mM; liquid stress N.D. -2.2 N.D. -0.1 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.5 N.D. -0.4 -0.2
solid stress Fraxetin 2 mM; solid stress N.D. -1.2 N.D. -0.1 -0.7
solid stress Fraxetin 3 mM; solid stress N.D. -1.3 N.D. -0.3 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 N.D. -0.5 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 N.D. -0.3 -0.8
solid stress Fraxetin 2 mM; solid stress N.D. -1.0 N.D. -0.5 -0.1
liquid stress Fraxetin 3 mM; liquid stress N.D. -1.7 N.D. +0.1 -0.0
solid stress Fraxetin 3 mM; solid stress N.D. -1.1 N.D. -0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 N.D. -0.5 -0.0
liquid stress Fraxetin 3 mM; liquid stress N.D. -1.7 N.D. -0.1 +0.2
solid stress Fraxetin 2 mM; solid stress N.D. -1.5 N.D. -0.1 -0.1
soil soil sample 7; outgrowth in LB N.D. -0.4 N.D. -0.7 -0.4
solid stress Fraxetin 2 mM; solid stress N.D. -0.9 N.D. -0.5 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.8 N.D. -0.6 -0.2
stress Polymyxin B sulfate 0.006 mg/ml N.D. -0.5 N.D. -0.5 -0.5
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.7 N.D. -0.2 -0.5
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.2 N.D. -0.2 -1.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.1 N.D. -1.0 -0.3
motility outer cut, LB soft agar motility assay N.D. -0.8 N.D. -0.2 -0.4
liquid stress Fraxetin 3 mM; liquid stress N.D. -1.3 N.D. +0.1 -0.1
solid stress Fraxetin 2 mM; solid stress N.D. -1.2 N.D. +0.1 -0.1
liquid stress Fraxetin 3 mM; liquid stress N.D. -1.3 N.D. +0.2 +0.2
stress Gentamicin 0.008 mg/ml N.D. +0.7 N.D. +0.7 -0.0
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +1.2 N.D. +0.1 +0.0
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