Fitness for 5 genes in Escherichia coli BW25113

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500 ntygfA and rygC are separated by 59 nucleotidesrygC and ibsC overlap by 99 nucleotidesibsC and serA are separated by 228 nucleotidesserA and rpiA are separated by 255 nucleotides b2912: ygfA - putative ligase (VIMSS), at 3,054,263 to 3,054,811 ygfA b4446: rygC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,054,871 to 3,055,010 rygC b4665: ibsC - toxic membrane protein (RefSeq), at 3,054,912 to 3,054,971 ibsC b2913: serA - D-3-phosphoglycerate dehydrogenase (NCBI), at 3,055,200 to 3,056,432 serA b2914: rpiA - ribose-5-phosphate isomerase A (NCBI), at 3,056,688 to 3,057,347 rpiA
Group Condition b2912 b4446 b4665 b2913 b2914
ygfA rygC ibsC serA rpiA
carbon source Ying_sugar7 rep B; time point 3 -5.9 N.D. N.D. -7.9 N.D.
carbon source Ying_sugar7 rep A; time point 3 -5.3 N.D. N.D. -7.9 N.D.
carbon source Ying_sugar7 rep C; time point 2 -4.0 N.D. N.D. -8.1 N.D.
carbon source Ying_OrganicAcid7 rep A; time point 3 -4.1 N.D. N.D. -7.9 N.D.
carbon source Ying_sugar7 rep C; time point 3 -4.2 N.D. N.D. -7.6 N.D.
carbon source Ying_sugar7 rep A; time point 2 -3.9 N.D. N.D. -7.8 N.D.
carbon source Ying_all64 rep B; time point 3 -5.2 N.D. N.D. -6.5 N.D.
carbon source Ying_all64 rep A; time point 3 -5.4 N.D. N.D. -6.1 N.D.
carbon source Ying_sugar7 rep B; time point 2 -3.5 N.D. N.D. -7.8 N.D.
carbon source Ying_OrganicAcid7 rep C; time point 3 -3.6 N.D. N.D. -7.6 N.D.
carbon source Ying_OrganicAcid7 rep B; time point 3 -3.2 N.D. N.D. -7.6 N.D.
carbon source Ying_OrganicAcid7 rep A; time point 2 -2.5 N.D. N.D. -7.4 N.D.
stress M9 with Methotrexate 0.06 mM 24 hr -2.0 N.D. N.D. -7.5 N.D.
carbon source Ying_Nucleobase14 rep A; time point 3 -1.3 N.D. N.D. -7.9 N.D.
carbon source Ying_all64 rep C; time point 3 -5.2 N.D. N.D. -3.9 N.D.
carbon source Ying_sugar7 rep C; time point 1 -3.7 N.D. N.D. -5.1 N.D.
carbon source Ying_Nucleobase14 rep C; time point 3 -0.9 N.D. N.D. -7.9 N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -2.2 N.D. N.D. -6.5 N.D.
stress M9 with Methotrexate 0.06 mM 48 hr -1.6 N.D. N.D. -7.0 N.D.
carbon source Ying_Nucleobase14 rep B; time point 2 -0.7 N.D. N.D. -7.8 N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 -0.7 N.D. N.D. -7.7 N.D.
carbon source Ying_OrganicAcid7 rep B; time point 2 -1.9 N.D. N.D. -6.4 N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -1.9 N.D. N.D. -6.4 N.D.
carbon source Ying_Nucleobase14 rep A; time point 2 -0.9 N.D. N.D. -7.3 N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -1.8 N.D. N.D. -6.3 N.D.
carbon source Ying_OrganicAcid7 rep C; time point 2 -1.7 N.D. N.D. -6.4 N.D.
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -3.0 N.D. N.D. -4.9 N.D.
stress M9 with Methotrexate 0.06 mM 48 hr -2.3 N.D. N.D. -5.6 N.D.
carbon source Ying_sugar7 rep B; time point 1 -3.0 N.D. N.D. -4.7 N.D.
carbon source carbon source D-Mannose 20mM +1.3 N.D. N.D. -7.6 N.D.
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