Fitness for 5 genes in Escherichia coli BW25113

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500 ntyciZ and gmr are separated by 147 nucleotidesgmr and ymiB are separated by 53 nucleotidesymiB and rnb are separated by 77 nucleotidesrnb and yciW are separated by 67 nucleotides b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633 yciZ b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766 gmr b4672: ymiB - expressed protein (RefSeq), at 1,344,820 to 1,344,924 ymiB b1286: rnb - exoribonuclease II (NCBI), at 1,345,002 to 1,346,936 rnb b1287: yciW - putative oxidoreductase (VIMSS), at 1,347,004 to 1,348,131 yciW
Group Condition b4596 b1285 b4672 b1286 b1287
yciZ gmr ymiB rnb yciW
carbon source carbon source D-Mannose 0.5mM -4.0 -2.4 N.D. -3.1 -1.3
predation 6 hr rep1_HD100_10fold_dilution_grewON -4.7 -0.3 N.D. -4.6 -0.6
carbon source Ying_sugar7 rep C; time point 3 -0.4 -7.5 N.D. -1.8 -0.4
carbon source Ying_sugar7 rep B; time point 3 +0.0 -7.6 N.D. -2.0 -0.0
carbon source Ying_sugar7 rep A; time point 3 +0.0 -6.3 N.D. -2.1 -0.1
carbon source Ying_sugar7 rep C; time point 2 +0.1 -6.2 N.D. -2.0 +0.3
carbon source Ying_sugar7 rep A; time point 2 -0.2 -5.2 N.D. -1.5 -0.1
carbon source Ying_sugar7 rep B; time point 2 -0.2 -4.4 N.D. -2.3 -0.1
carbon source carbon source D-Mannose 1mM -4.0 -1.9 N.D. -1.0 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 -2.7 -0.7 N.D. -3.0 +0.0
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.3 -0.8 N.D. -4.2 -0.3
carbon source Ying_sugar7 rep A; time point 1 -0.3 -4.6 N.D. -0.9 +0.2
carbon source Ying_sugar7 rep C; time point 1 -0.3 -3.7 N.D. -1.1 -0.1
carbon source Ying_sugar7 rep B; time point 1 +0.1 -4.3 N.D. -0.7 +0.0
carbon source carbon source D-Mannose 0.5mM +0.2 -1.7 N.D. -2.7 +0.1
motility outer cut, LB soft agar motility assay +1.1 -0.3 N.D. -4.2 -0.2
stress decyl alcohol 5 vol% +0.0 -0.1 N.D. -3.8 +0.3
motility outer cut, LB soft agar motility assay +0.5 +0.1 N.D. -4.5 +0.3
phage TP4 -1.5 -0.4 N.D. -2.0 +0.6
stress Tetracycline 0.0004 mg/ml +0.5 -0.0 N.D. -3.7 +0.1
gfp_control GFP_control +1.1 -0.2 N.D. -2.9 -0.9
carbon source carbon source D-Mannose 5mM +1.0 -2.0 N.D. -1.7 -0.0
carbon source Ying_Nucleobase14 rep B; time point 2 +0.6 -3.0 N.D. -0.5 +0.2
carbon source Ying_Nucleobase14 rep B; time point 3 +1.2 -4.3 N.D. +0.6 +0.1
carbon source Ying_Nucleobase14 rep C; time point 3 +1.0 -2.9 N.D. +0.4 -0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.9 -2.2 N.D. +1.2 -0.5
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +1.3 -1.5 N.D. +0.9 -1.1
plasmid transformation vcDART vector transformation; agar assay -0.8 -0.1 N.D. +3.3 -0.3
plasmid transformation vcDART vector transformation; agar assay +0.3 -0.0 N.D. +3.7 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 -0.4 N.D. +4.1 +0.3
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