Fitness for 5 genes in Escherichia coli BW25113

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500 ntmcrB and yjiW are separated by 161 nucleotidesyjiW and ryjC overlap by 6 nucleotidesryjC and hsdS are separated by 156 nucleotideshsdS and hsdM overlap by 4 nucleotides b4346: mcrB - component of McrBC 5-methylcytosine restriction system (VIMSS), at 4,575,981 to 4,577,360 mcrB b4347: yjiW - conserved protein (RefSeq), at 4,577,522 to 4,577,863 yjiW b4625: ryjC - Novel sRNA, function unknown (RefSeq), at 4,577,858 to 4,577,934 ryjC b4348: hsdS - specificity determinant for hsdM and hsdR (NCBI), at 4,578,091 to 4,579,485 hsdS b4349: hsdM - DNA methylase M (NCBI), at 4,579,482 to 4,581,071 hsdM
Group Condition b4346 b4347 b4625 b4348 b4349
mcrB yjiW ryjC hsdS hsdM
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.3 -0.2 -1.1 -1.6
M9 Glucose 2% aerobic -1.3 -0.0 -0.1 -0.5 -0.1
M9 Acetate 0.5% aerobic -1.3 +0.0 +0.0 -0.6 -0.0
carbon source carbon source D-Glucose 0.25mM -0.5 -0.0 -0.4 -0.8 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.1 -0.2 -1.1 -0.8
M9 Acetate 0.5% aerobic -1.1 +0.1 -0.1 -0.6 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 +0.2 -0.3 -0.8 -1.0
M9 Glucose 2% aerobic -1.1 -0.0 -0.1 -0.5 -0.0
M9 Glucose 2%, 20 mM NO3- anaerobic -1.2 +0.1 -0.0 -0.5 -0.1
M9 Glucose 2% anaerobic -1.1 +0.1 -0.0 -0.6 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.2 -0.2 -0.5 -0.8
stress Cisplatin 0.1 mg/ml -0.1 -0.5 +0.1 -0.5 -0.5
motility inner cut, LB soft agar motility assay -0.7 -0.2 +0.0 -0.7 +0.0
phage Ste41 +0.5 -0.4 -0.6 -0.3 -0.3
motility outer cut, LB soft agar motility assay -0.6 +0.4 +0.1 -0.6 -0.3
carbon source carbon source D-Mannose 5mM +0.0 +0.5 -0.7 -0.3 -0.3
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.5 -0.4 -0.4 +0.4 -0.1
phage Δdbn_10 +0.7 -0.2 -0.0 +0.2 -0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 +0.4 +0.6 -0.2 -0.5
stress LB Aerobic with Irgasan 0.0000003125 mM +0.7 -0.5 +0.2 +0.4 -0.2
phage T7_phage 0.00015625 MOI TC4 +0.6 -0.6 +0.6 +0.3 -0.1
phage T7_phage 0.0015625 MOI TC2 +0.8 +0.1 -0.4 +0.4 -0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.9 -0.5 +0.1 +0.2 +0.2
control PBS_cage_1_mice _Feces_22 hr +0.5 -0.4 +0.3 +0.2 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.8 +0.1 +0.2 +0.3 -0.4
phage N4_phage 0.009375 MOI TC2 +1.3 +0.2 -0.2 +0.3 +0.1
phage Papa dilution 10-1 cacl2 maltose +1.5 -0.2 -0.0 +0.6 +0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.6 -0.1 +0.9 +0.5 -0.1
phage Lambda_ci1857 +0.4 +0.2 +0.0 +0.8 +0.6
control PBS_cage_1_mouse_1_Cecum_40 hr +0.9 +0.5 +0.3 +0.6 -0.0
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