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Cofit
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
sgcE and sgcA are separated by 11 nucleotides
sgcA and ryjB overlap by 4 nucleotides
ryjB and sgcQ are separated by 44 nucleotides
sgcQ and sgcC are separated by 12 nucleotides
b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560
sgcE
b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003
sgcA
b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089
ryjB
b4303: sgcQ - KpLE2 phage-like element; predicted nucleoside triphosphatase (NCBI), at 4,526,134 to 4,526,940
sgcQ
b4304: sgcC - KpLE2 phage-like element; predicted phosphotransferase enzyme IIC component (NCBI), at 4,526,953 to 4,528,266
sgcC
Group
Condition
b4301
b4302
b4624
b4303
b4304
sgcE
sgcA
ryjB
sgcQ
sgcC
carbon source
carbon source D-Glucose 5mM
-0.0
-1.0
N.D.
-0.7
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.7
-0.1
N.D.
-0.1
-0.7
phage
Papa dilution 10-1 cacl2 maltose
-0.5
-0.5
N.D.
-0.2
-0.3
phage
T7_phage 0.00015625 MOI TC4
-0.7
-0.3
N.D.
-0.3
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
+0.1
N.D.
-0.5
-0.7
phage
Bas60
-0.6
-0.1
N.D.
-0.3
-0.2
nophagecontrol
R1_N4_T1_phage0
-0.4
-0.5
N.D.
-0.3
+0.1
phage
Δdbn_10
-0.0
-0.0
N.D.
-1.1
+0.1
phage
T7_phage 0.00015625 MOI TC3
-0.5
-0.3
N.D.
-0.1
-0.2
phage
T7_phage 0.0015625 MOI TC2
+0.2
-0.2
N.D.
-0.7
-0.2
stress
3-methyl-1-butanol 1.25 vol%
-0.6
+0.1
N.D.
-0.4
-0.2
stress
Cisplatin 0.1 mg/ml
-0.5
-0.7
N.D.
+0.5
+0.1
gfp_control
GFP_control
-0.7
+0.2
N.D.
+0.1
-0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.3
+0.3
N.D.
-0.3
-0.2
mzaabcde
mzaABCDE
-0.4
+0.4
N.D.
-0.1
-0.2
drt_type_3
DRT_type_3
-0.4
+0.4
N.D.
+0.1
-0.2
phage
Ste41
+0.4
+0.3
N.D.
-0.7
+0.1
phage
R2_N4_T1_phage10
-0.1
+0.4
N.D.
-0.4
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.3
+0.4
N.D.
-0.3
+0.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.3
-0.5
N.D.
+0.1
+0.2
phage
RB68
+0.3
+0.4
N.D.
-0.3
-0.2
control
PBS_cage_1_mice _Feces_16 hr
+0.5
+0.3
N.D.
-0.5
+0.0
motility
inner cut, LB soft agar motility assay
+0.2
-0.4
N.D.
+0.4
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.6
+0.3
N.D.
+0.8
-0.1
motility
outer cut, LB soft agar motility assay
+0.3
+0.6
N.D.
-0.3
-0.0
control
PBS_cage_2_mouse_3_Cecum_40 hr
+0.6
-0.4
N.D.
+0.4
+0.1
phage
TP4
+0.3
+0.4
N.D.
+0.3
-0.2
motility
inner cut, LB soft agar motility assay
+0.4
-0.2
N.D.
+0.6
+0.2
motility
inner cut, LB soft agar motility assay
+0.8
-0.3
N.D.
+0.5
+0.1
phage
Koxy_10
+0.3
+0.3
N.D.
+0.4
+0.3
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