Fitness for 5 genes in Escherichia coli BW25113

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500 ntcadB and cadC are separated by 364 nucleotidescadC and yjdQ are separated by 256 nucleotidesyjdQ and pheU are separated by 197 nucleotidespheU and yjdC are separated by 106 nucleotides b4132: cadB - predicted lysine/cadaverine transporter (NCBI), at 4,356,720 to 4,358,054 cadB b4133: cadC - DNA-binding transcriptional activator (NCBI), at 4,358,419 to 4,359,957 cadC b4654: yjdQ - no description, at 4,360,214 to 4,360,376 yjdQ b4134: pheU - tRNA-Phe (NCBI), at 4,360,574 to 4,360,649 pheU b4135: yjdC - orf, hypothetical protein (VIMSS), at 4,360,756 to 4,361,331 yjdC
Group Condition b4132 b4133 b4654 b4134 b4135
cadB cadC yjdQ pheU yjdC
stress LB + ciprofloxacin regrowth after 48hrs -1.1 -0.6 -0.7 N.D. -0.2
lb LB regrowth after 48hrs -1.0 -0.6 -0.7 N.D. -0.2
lb LB regrowth after 48hrs -1.0 -0.6 -0.5 N.D. -0.3
stress LB + carbenicillin regrowth after 48hr -0.9 -0.5 -0.5 N.D. -0.2
M9 Acetate 0.5% aerobic -0.5 -0.9 +0.1 N.D. +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.4 +0.7 N.D. -0.5
stress BHIS with Methotrexate 1.98 mM 48 hr +0.0 +0.9 -1.2 N.D. -0.0
stress BHIS with Methotrexate 1.98 mM 24 hr +0.1 +1.0 -1.3 N.D. +0.0
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.2 +1.0 -1.3 N.D. -0.0
stress BHIS with Methotrexate 0.06 mM 24 hr +0.1 +1.1 -1.2 N.D. -0.1
stress BHIS with Methotrexate 0.1 mM 48 hr +0.2 +1.0 -1.1 N.D. -0.1
stress BHIS with Methotrexate 1.98 mM 48 hr +0.1 +1.0 -1.2 N.D. +0.1
stress BHIS with Methotrexate 1.98 mM 48 hr +0.1 +0.9 -1.0 N.D. +0.0
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.1 +1.0 -1.2 N.D. +0.1
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.1 +1.0 -1.2 N.D. +0.1
stress BHIS with Methotrexate 0.06 mM 48 hr +0.1 +1.0 -1.3 N.D. +0.1
stress BHIS with Methotrexate 0.1 mM 24 hr +0.1 +1.1 -1.2 N.D. +0.0
stress BHIS with Methotrexate 0.1 mM 24 hr +0.1 +1.0 -1.1 N.D. +0.1
stress BHIS with Methotrexate 1.98 mM 24 hr +0.1 +1.1 -1.1 N.D. +0.0
stress BHIS with Methotrexate 0.06 mM 48 hr +0.1 +1.1 -1.2 N.D. +0.0
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.2 +1.0 -1.3 N.D. +0.2
stress BHIS with Methotrexate 0.1 mM 48 hr +0.2 +1.0 -1.2 N.D. +0.1
stress BHIS with Methotrexate 0.06 mM 24 hr +0.1 +1.1 -1.1 N.D. +0.1
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.2 +1.1 -1.2 N.D. +0.1
stress BHIS with Methotrexate 1.98 mM 24 hr +0.0 +1.1 -1.0 N.D. +0.1
stress BHIS with Methotrexate 0.06 mM 24 hr +0.2 +1.1 -1.2 N.D. +0.1
stress BHIS with Methotrexate 0.1 mM 24 hr +0.2 +1.0 -1.0 N.D. +0.1
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.2 +1.1 -1.0 N.D. +0.2
stress BHIS with Methotrexate 0.1 mM 48 hr +0.2 +1.1 -0.7 N.D. +0.1
phage T7_phage 0.0015625 MOI TC2 +0.1 +0.7 +1.0 N.D. +0.2
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