Fitness for 5 genes in Escherichia coli BW25113

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500 ntrrsG and clpB are separated by 442 nucleotidesclpB and ryfD overlap by 21 nucleotidesryfD and yfiH are separated by 7 nucleotidesyfiH and rluD overlap by 4 nucleotides b2591: rrsG - 16S ribosomal RNA (NCBI), at 2,727,638 to 2,729,179 rrsG b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317 ryfD b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056 yfiH b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD
Group Condition b2591 b2592 b4609 b2593 b2594
rrsG clpB ryfD yfiH rluD
stress Trimethoprim 0.00125 mM N.D. -0.2 -1.5 -1.2 N.D.
stress Aluminum chloride 5 mM N.D. -1.0 +0.0 -1.9 N.D.
phage N4_phage 0.09375 MOI TC1 N.D. -1.0 -0.5 -0.6 N.D.
stress Cephalothin 0.01 mg/ml N.D. -0.3 +0.1 -2.0 N.D.
phage N4_phage 0.0009375 MOI TC2 N.D. -0.7 -0.9 -0.4 N.D.
stress 3-methyl-1-butanol 1.25 vol% N.D. -0.6 -0.3 -1.0 N.D.
phage N4_phage 0.0009375 MOI TC3 N.D. -0.5 -1.2 -0.2 N.D.
phage Δdbn_10 N.D. +0.3 -1.3 -0.9 N.D.
phage N4_phage 0.00009375 MOI TC4 N.D. -0.5 -0.9 -0.5 N.D.
stress 3-methyl-1-butanol 1.25 vol% N.D. -0.4 -0.8 -0.6 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.1 -0.6 -1.2 N.D.
mzaabcde mzaABCDE N.D. -0.2 -1.3 -0.2 N.D.
phage Ste41 N.D. -0.3 -1.5 +0.3 N.D.
stress Cisplatin 0.05 mg/ml N.D. +0.1 -0.8 -0.8 N.D.
stress Carbenicillin 0.004 mg/ml N.D. +0.1 +0.3 -1.7 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.1 +0.6 -1.3 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.3 +0.3 -1.2 N.D.
stress Cisplatin 0.1 mg/ml N.D. +0.1 +0.6 -1.1 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.2 +0.4 -1.1 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.2 +0.6 -0.8 N.D.
carbon source Glycolic Acid (C) N.D. -0.4 +0.9 -0.5 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.1 +0.9 -0.7 N.D.
stress Doxycycline hyclate 0.001 mg/ml N.D. -0.4 -0.6 +1.1 N.D.
stress Trimethoprim 0.005 mM N.D. -0.2 +1.3 -0.4 N.D.
phage T7_phage 0.00015625 MOI TC3 N.D. -0.0 -0.7 +1.4 N.D.
stress limonene 5 vol% N.D. +0.9 +0.7 -0.4 N.D.
stress octanol 0.08 vol% N.D. +0.2 -0.5 +1.8 N.D.
stress limonene 5 vol% N.D. +1.1 +0.6 -0.0 N.D.
stress Chloramphenicol 0.001 mg/ml N.D. +0.1 +0.5 +1.1 N.D.
stress Bacitracin 1 mg/ml N.D. +0.2 +0.6 +1.9 N.D.
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