Fitness for 5 genes in Escherichia coli BW25113

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500 ntnapD and napF overlap by 11 nucleotidesnapF and yojO are separated by 108 nucleotidesyojO and eco are separated by 134 nucleotideseco and mqo are separated by 714 nucleotides b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035 napD b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519 napF b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792 yojO b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415 eco b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776 mqo
Group Condition b2207 b2208 b4604 b2209 b2210
napD napF yojO eco mqo
control PBS_cage_2_mouse_1_Cecum_40 hr +0.2 +0.1 N.D. -6.9 +0.0
carbon source carbon source D-Mannose 5mM +0.3 +0.1 N.D. -5.4 +0.0
carbon source carbon source D-Glucose 0.25mM -0.5 -1.3 N.D. -1.4 -0.3
phage Δdbn_10 -0.0 +0.2 N.D. -2.9 -0.2
phage T7_phage 0.00015625 MOI TC3 -0.7 -1.5 N.D. -0.0 -0.5
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.2 -0.4 N.D. -1.8 -0.1
phage RB68 +0.0 -0.1 N.D. -1.6 -0.4
motility outer cut, LB soft agar motility assay +0.1 -0.1 N.D. -1.9 -0.1
phage RB68 +0.2 -0.4 N.D. -1.5 -0.3
stress 3-methyl-1-butanol 1.25 vol% -0.5 -0.9 N.D. +0.5 -0.5
phage Phage_N4_cage_4_mice _Feces_16 hr +0.4 -0.3 N.D. -1.4 -0.1
carbon source Guanosine 0.35306 mM (C) +0.3 +0.2 N.D. -1.9 -0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 +0.6 N.D. -1.3 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.9 -0.8 N.D. +1.7 -0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.3 -0.5 N.D. -1.1 +0.2
carbon source carbon source D-Mannose 0.5mM -1.0 -0.4 N.D. +0.8 -0.2
protonated/deuterated deuterated glucose in H2O -0.8 -0.7 N.D. +0.6 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.3 N.D. -1.7 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 +0.3 N.D. -1.3 +0.6
phage T7_phage 0.0015625 MOI TC1 +0.7 +0.2 N.D. -1.2 -0.2
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.1 +0.9 N.D. -1.0 -0.1
mzaabcde mzaABCDE -0.8 -0.5 N.D. +0.8 +0.2
stress ethyl acetate 5 vol% -0.6 -0.2 N.D. +1.1 -0.4
phage T7_phage 0.00015625 MOI TC4 +1.1 +0.5 N.D. -1.5 -0.1
stress octanol 0.08 vol% -1.4 +0.1 N.D. +1.2 +0.3
carbon source carbon source D-Mannose 1mM -0.5 -0.4 N.D. +1.7 -0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.4 -0.6 N.D. +1.3 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.5 +0.8 N.D. -0.4 +0.6
stress benzenesulfonyl fluoride +0.5 +0.3 N.D. +1.2 +0.2
carbon source carbon source D-Glucose 0.25mM +0.2 +0.7 N.D. +1.4 +0.1
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