Fitness for 5 genes in Escherichia coli BW25113

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500 ntsodB and ydhP are separated by 161 nucleotidesydhP and ynhF are separated by 165 nucleotidesynhF and purR are separated by 298 nucleotidespurR and ydhB overlap by 4 nucleotides b1656: sodB - superoxide dismutase, Fe (NCBI), at 1,733,402 to 1,733,983 sodB b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314 ydhP b4602: ynhF - hypothetical protein (RefSeq), at 1,735,480 to 1,735,569 ynhF b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893 purR b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822 ydhB
Group Condition b1656 b1657 b4602 b1658 b1659
sodB ydhP ynhF purR ydhB
stress Benzalkonium Chloride 0.004 mg/ml +0.3 +0.3 N.D. -3.9 -2.1
motility outer cut, LB soft agar motility assay -0.3 -0.7 N.D. -2.8 -1.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.0 +0.1 N.D. -3.9 -0.1
stress Trimethoprim 0.0025 mM -0.4 -0.2 N.D. -3.3 -0.4
carbon source Ying_Nucleobase14 rep B; time point 3 -0.5 +0.4 N.D. -1.2 -2.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.3 -0.2 N.D. -2.1 -0.8
stress Trimethoprim 0.00125 mM -0.6 +0.0 N.D. -2.7 +0.3
stress Cephalothin 0.01 mg/ml -0.4 -0.5 N.D. -1.8 -0.3
carbon source Ying_Nucleobase14 rep C; time point 2 -0.6 +0.2 N.D. -1.1 -1.4
stress 2,4-Dinitrophenol 2 mM +0.3 +0.0 N.D. -2.6 -0.4
phage Lambda_ci1857 +0.4 -0.2 N.D. -2.0 -0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.6 +0.2 N.D. -3.4 -0.0
carbon source carbon source D-Mannose 0.5mM -1.8 -0.6 N.D. -0.6 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.2 +0.1 N.D. -2.4 -0.3
stress octanol 0.08 vol% +0.5 -0.4 N.D. -3.5 +0.9
carbon source D-Serine (C) -2.9 -0.3 N.D. +0.8 -0.1
phage RB68 -1.2 +0.4 N.D. -2.2 +0.6
stress Cholin acetate 75 mM -0.3 +0.4 N.D. -2.3 -0.1
phage Ste41 +0.4 -0.1 N.D. -2.3 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.9 +0.1 N.D. -1.9 -0.6
carbon source carbon source D-Glucose 0.25mM +0.8 -0.9 N.D. -0.6 -0.8
motility inner cut, LB soft agar motility assay +0.9 -0.4 N.D. -1.7 +0.3
nitrogen source Cytidine (N) -1.8 +0.4 N.D. +0.7 +0.3
stress Chlorite 1.25 mM +1.0 +0.3 N.D. -1.9 +0.1
phage Ste41 -0.3 -0.5 N.D. -1.3 +2.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.6 +0.3 N.D. +1.6 +1.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 -0.1 N.D. +2.0 -1.0
plasmid transformation vcDART vector transformation; agar assay +0.2 +0.3 N.D. +2.5 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.1 N.D. +2.8 +0.2
plasmid transformation vcDART vector transformation; agar assay +0.4 +0.2 N.D. +2.8 -0.3
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