Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjiS and yjiT are separated by 498 nucleotidesyjiT and yjiV are separated by 2 nucleotidesyjiV and mcrC are separated by 56 nucleotidesmcrC and mcrB overlap by 1 nucleotides b4341: yjiS - hypothetical protein (NCBI), at 4,569,774 to 4,569,938 yjiS b4342: yjiT - orf, hypothetical protein (VIMSS), at 4,570,437 to 4,571,939 yjiT b4486: yjiV - conserved protein (pseudogene) (RefSeq), at 4,571,942 to 4,574,878 yjiV b4345: mcrC - 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI), at 4,574,935 to 4,575,981 mcrC b4346: mcrB - component of McrBC 5-methylcytosine restriction system (VIMSS), at 4,575,981 to 4,577,360 mcrB
Group Condition b4341 b4342 b4486 b4345 b4346
yjiS yjiT yjiV mcrC mcrB
M9 Glucose 2%, 20 mM NO3- anaerobic -0.6 -0.4 +0.0 -1.5 -1.2
M9 Acetate 0.5% aerobic -0.8 -0.5 +0.1 -1.3 -1.1
M9 Glucose 2% aerobic -0.8 -0.4 +0.0 -1.1 -1.3
M9 Acetate 0.5% aerobic -1.2 -0.5 +0.2 -0.7 -1.3
M9 Glucose 2% aerobic -0.6 -0.4 +0.0 -1.3 -1.1
M9 Glucose 2% anaerobic -0.7 -0.4 +0.0 -1.0 -1.1
motility outer cut, LB soft agar motility assay -1.1 -0.3 +0.1 -1.0 -0.6
motility inner cut, LB soft agar motility assay -0.2 -0.2 +0.0 -1.7 -0.5
motility inner cut, LB soft agar motility assay -0.3 -0.2 +0.1 -1.3 -0.7
carbon source carbon source D-Glucose 1mM -1.0 +0.0 -0.2 -1.0 -0.1
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.7 +0.1 +0.1 -1.3 -0.4
M9 Glucose 2% anaerobic -0.4 -0.3 +0.1 -0.6 -0.8
motility outer cut, LB soft agar motility assay +0.1 +0.1 +0.0 -1.5 -0.6
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.3 +0.3 +0.0 -1.7 -0.4
carbon source carbon source D-Glucose 0.25mM -2.0 -0.0 -0.0 +0.9 -0.3
phage Ste41 -1.7 +0.1 +0.1 +1.6 +0.5
control PBS_cage_1_mouse_1_Cecum_40 hr +0.3 +0.4 +0.4 -0.9 +0.9
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.5 -0.0 -0.2 +1.3 +0.5
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.3 -0.1 +0.0 +1.2 +0.8
stress Ciprofloxacin Hydrochloride 0.000156 mM -0.5 +0.1 -0.1 +1.6 +0.5
control PBS_cage_2_mouse_2_Cecum_40 hr +0.7 +0.2 +0.2 +1.0 -0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.4 +0.3 +0.1 +1.3 +0.6
phage Δdbn_10 -0.4 -0.0 -0.1 +1.7 +0.7
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 +0.3 -0.2 +0.7 +0.9
stress Trimethoprim 0.005 mM -0.3 +0.2 -0.1 +1.5 +0.8
phage T7_phage 0.0015625 MOI TC2 +0.2 +0.4 -0.5 +1.6 +0.8
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.2 +0.3 +0.4 +2.1 -0.1
phage N4_phage 0.09375 MOI TC1 +0.0 +0.1 -0.1 +2.2 +0.7
phage Papa dilution 10-1 cacl2 maltose -0.2 -0.0 -0.3 +2.3 +1.5
phage N4_phage 0.009375 MOI TC2 +0.3 +0.1 -0.0 +2.4 +1.3
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