Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjgD and yjgM are separated by 144 nucleotidesyjgM and yjgN are separated by 192 nucleotidesyjgN and valS are separated by 55 nucleotidesvalS and holC overlap by 1 nucleotides b4255: yjgD - hypothetical protein (NCBI), at 4,476,496 to 4,476,912 yjgD b4256: yjgM - predicted acetyltransferase (NCBI), at 4,477,057 to 4,477,560 yjgM b4257: yjgN - conserved inner membrane protein (NCBI), at 4,477,753 to 4,478,949 yjgN b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860 valS b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303 holC
Group Condition b4255 b4256 b4257 b4258 b4259
yjgD yjgM yjgN valS holC
carbon source Glycolic Acid (C) -0.9 -1.3 +0.1 N.D. N.D.
carbon source Glycolic Acid (C) -0.3 -1.4 +0.0 N.D. N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON -2.0 +0.1 +0.4 N.D. N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -1.4 -0.1 +0.2 N.D. N.D.
stress Bacitracin 1 mg/ml -1.6 +0.3 -0.0 N.D. N.D.
motility outer cut, LB soft agar motility assay -1.0 +0.2 -0.4 N.D. N.D.
carbon source acetate (C) +0.2 -1.5 +0.3 N.D. N.D.
stress Spectinomycin 0.0125 mg/ml -1.2 +0.2 +0.1 N.D. N.D.
carbon source acetate (C) +0.3 -1.2 +0.2 N.D. N.D.
stress Cephalothin 0.01 mg/ml -1.5 +0.5 +0.4 N.D. N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM -0.7 -0.3 +0.4 N.D. N.D.
M9 Acetate 0.5% aerobic +0.4 -0.9 -0.1 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 +0.0 -0.9 N.D. N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr -1.0 +0.2 +0.6 N.D. N.D.
stress Benzalkonium Chloride 0.004 mg/ml +0.7 -0.1 -0.7 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.0 +0.1 -1.0 N.D. N.D.
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.6 +0.5 -0.8 N.D. N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +1.3 -0.1 -0.2 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.3 +0.1 -0.2 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.2 +0.3 -0.3 N.D. N.D.
carbon source Inosine 0.3728 mM (C) +0.7 +0.7 -0.1 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +1.6 -0.0 -0.3 N.D. N.D.
phage T7_phage 0.0015625 MOI TC2 +0.6 +0.3 +0.5 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 2 +0.4 +0.9 +0.1 N.D. N.D.
carbon source Ying_Nucleobase14 rep C; time point 2 +0.3 +0.9 +0.3 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 2 +0.4 +0.9 +0.2 N.D. N.D.
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.8 +0.3 +0.4 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 3 +0.4 +1.2 +0.1 N.D. N.D.
carbon source Ying_Nucleobase14 rep C; time point 3 +0.4 +1.1 +0.2 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 +0.8 +1.0 +0.2 N.D. N.D.
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