Fitness for 5 genes in Escherichia coli BW25113

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500 ntalaT and rrlA are separated by 183 nucleotidesrrlA and rrfA are separated by 93 nucleotidesrrfA and mobB are separated by 269 nucleotidesmobB and mobA overlap by 19 nucleotides b3853: alaT - tRNA-Ala (NCBI), at 4,035,283 to 4,035,358 alaT b3854: rrlA - 23S ribosomal RNA (NCBI), at 4,035,542 to 4,038,446 rrlA b3855: rrfA - 5S ribosomal RNA (NCBI), at 4,038,540 to 4,038,659 rrfA b3856: mobB - molybdopterin-guanine dinucleotide biosynthesis protein B (RefSeq), at 4,038,929 to 4,039,456 mobB b3857: mobA - molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI), at 4,039,438 to 4,040,022 mobA
Group Condition b3853 b3854 b3855 b3856 b3857
alaT rrlA rrfA mobB mobA
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM N.D. N.D. N.D. +0.5 -3.0
stress 2,4-Dinitrophenol 2 mM N.D. N.D. N.D. -0.2 -2.1
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. N.D. +0.3 -2.5
stress LB Anaerobic with Hygromycin B 0.00025 mM N.D. N.D. N.D. -0.0 -2.1
stress 2,4-Dinitrophenol 2 mM N.D. N.D. N.D. -0.0 -1.8
stress octanol 0.08 vol% N.D. N.D. N.D. +0.1 -1.7
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. N.D. -0.1 -1.5
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM N.D. N.D. N.D. +0.5 -2.1
stress LB Anaerobic with Oxytetracycline 0.0000003125 mM N.D. N.D. N.D. -0.2 -1.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. N.D. N.D. -0.3 -1.3
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM N.D. N.D. N.D. +0.1 -1.6
control PBS_cage_1_mice _Feces_40 hr N.D. N.D. N.D. +0.1 -1.5
M9 Glucose 2%, 20 mM NO3- anaerobic N.D. N.D. N.D. +0.2 -1.5
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. N.D. N.D. +0.2 -1.5
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr N.D. N.D. N.D. +0.3 -1.5
stress LB Anaerobic with Furaltadone 0.00000390625 mM N.D. N.D. N.D. +0.2 -1.5
control PBS_cage_1_mice _Feces_16 hr N.D. N.D. N.D. +0.2 -1.4
stress LB Anaerobic with Hygromycin B 0.00025 mM N.D. N.D. N.D. +0.3 -1.5
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM N.D. N.D. N.D. +0.3 -1.4
stress LB Anaerobic with Doxycycline hyclate 0.000000390625 mM N.D. N.D. N.D. +0.3 -1.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. N.D. N.D. +0.6 -1.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. N.D. -0.4 +1.4
stress Spectinomycin 0.00625 mg/ml N.D. N.D. N.D. -0.4 +1.8
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. +0.6 +1.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. N.D. +0.4 +1.4
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. N.D. N.D. +0.4 +1.5
stress Benzalkonium Chloride 0.004 mg/ml N.D. N.D. N.D. +0.0 +2.1
stress Spectinomycin 0.0125 mg/ml N.D. N.D. N.D. -0.3 +2.6
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -0.0 +2.4
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. +0.1 +2.6
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