Fitness for 5 genes in Escherichia coli BW25113

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500 ntrhtC and rhtB are separated by 61 nucleotidesrhtB and pldB are separated by 110 nucleotidespldB and yigL are separated by 7 nucleotidesyigL and yigM are separated by 75 nucleotides b3823: rhtC - threonine efflux system (NCBI), at 4,005,780 to 4,006,400 rhtC b3824: rhtB - neutral amino-acid efflux system (NCBI), at 4,006,462 to 4,007,082 rhtB b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215 pldB b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023 yigL b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998 yigM
Group Condition b3823 b3824 b3825 b3826 b3827
rhtC rhtB pldB yigL yigM
motility outer cut, LB soft agar motility assay -0.8 -0.4 -0.8 +0.1 -0.6
stress Spectinomycin 0.0125 mg/ml -0.3 -0.2 -1.0 -0.2 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.6 +0.0 -0.7 -0.3 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 -0.1 -1.0 -0.2 -0.3
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 -0.3 -0.4 +0.1 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -0.3 -0.0 -0.1 -0.1
lb anaerobic no stress control LB anaerobic no stress control -0.2 -0.1 -0.8 -0.1 -0.1
phage R1_N4_T2_phage8 -0.7 +0.1 -0.1 +0.3 -0.6
sulfur source M63 no sulfur + 5 mM L-cys -0.1 +0.0 -0.6 -0.1 -0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 +0.0 -0.9 +0.1 -0.2
phage T7_phage 0.00015625 MOI TC4 -0.3 -0.3 -0.6 -0.2 +0.4
phage phage -0.2 -0.1 -0.5 -0.3 +0.1
carbon source carbon source D-Mannose 5mM -0.2 -0.1 +0.2 -0.3 -0.5
stress Spectinomycin 0.00625 mg/ml +0.2 +0.1 -0.5 -0.2 -0.3
carbon source Glycolic Acid (C) +0.1 +0.2 -0.8 +0.2 -0.3
stress octanol 0.08 vol% +0.2 -0.7 -0.2 -0.3 +0.4
phage T7_phage 0.0015625 MOI TC2 +0.4 -0.4 -0.3 -0.3 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.2 +0.2 +0.1 -0.3
stress Cisplatin 0.1 mg/ml -0.3 +0.3 +0.3 -0.4 -0.4
stress Aluminum chloride 5 mM -0.3 +0.2 +0.1 -0.1 -0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 -0.1 +0.2 -0.3 -0.4
carbon source acetate (C) -0.2 +0.1 +0.5 -0.2 -0.4
phage Laval1267 +0.0 -0.2 +0.5 -0.4 +0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -0.3 +0.7 +0.2 +0.2
stress Trimethoprim 0.00125 mM -0.2 -0.1 +0.5 +0.3 +0.2
phage Ste41 +0.8 +0.3 -0.3 -0.3 +0.5
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 +0.4 -0.6 +0.5 +0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.3 -0.1 +0.4 +0.1 +0.5
plasmid transformation mariner vector transformation; agar assay +0.1 +0.3 +0.3 +0.2 +0.3
control PBS_cage_1_mouse_1_Cecum_40 hr -0.1 +0.4 +0.1 +0.6 +0.5
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