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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
wzyE and rffM are separated by 2 nucleotides
rffM and yifK are separated by 190 nucleotides
yifK and argX are separated by 102 nucleotides
argX and hisR are separated by 57 nucleotides
b3793: wzyE - putative enterobacterial common antigen polymerase (NCBI), at 3,976,624 to 3,977,976
wzyE
b3794: rffM - putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI), at 3,977,979 to 3,978,719
rffM
b3795: yifK - predicted transporter (NCBI), at 3,978,910 to 3,980,295
yifK
b3796: argX - tRNA-Arg (NCBI), at 3,980,398 to 3,980,474
argX
b3797: hisR - tRNA-His (NCBI), at 3,980,532 to 3,980,608
hisR
Group
Condition
b3793
b3794
b3795
b3796
b3797
wzyE
rffM
yifK
argX
hisR
stress
octanol 0.08 vol%
N.D.
-5.9
-0.1
N.D.
N.D.
mzaabcde
mzaABCDE
N.D.
-5.5
-0.3
N.D.
N.D.
mzaabcde
mzaABCDE
N.D.
-5.3
-0.1
N.D.
N.D.
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
N.D.
-4.8
-0.3
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
N.D.
-4.7
-0.2
N.D.
N.D.
sir2-hera
SIR2-HerA
N.D.
-4.8
-0.0
N.D.
N.D.
ppi
ppI
N.D.
-4.5
-0.1
N.D.
N.D.
drt_type_3
DRT_type_3
N.D.
-4.6
-0.0
N.D.
N.D.
ppi
ppI
N.D.
-4.4
-0.1
N.D.
N.D.
micoeukaryotes
Tetrahymena sp., Mixed culture-1
N.D.
-4.5
+0.1
N.D.
N.D.
mzaabcde
mzaABCDE
N.D.
-4.2
-0.1
N.D.
N.D.
stress
Benzalkonium Chloride 0.004 mg/ml
N.D.
-3.4
-0.9
N.D.
N.D.
gfp_control
GFP_control
N.D.
-4.3
+0.1
N.D.
N.D.
retron_ec_48_control
retron_Ec_48_control
N.D.
-4.2
-0.0
N.D.
N.D.
drt_type_3
DRT_type_3
N.D.
-4.1
-0.0
N.D.
N.D.
stress
2,4-Dinitrophenol 2 mM
N.D.
-4.0
-0.1
N.D.
N.D.
carbon source
a-Ketoglutaric (C)
N.D.
-4.1
+0.1
N.D.
N.D.
sir2-hera
SIR2-HerA
N.D.
-3.9
-0.0
N.D.
N.D.
retron_ec_48_control
retron_Ec_48_control
N.D.
-3.9
-0.0
N.D.
N.D.
sir2-hera
SIR2-HerA
N.D.
-3.9
-0.1
N.D.
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
N.D.
-3.9
+0.0
N.D.
N.D.
stress
2,4-Dinitrophenol 2 mM
N.D.
-3.8
+0.1
N.D.
N.D.
gfp_control
GFP_control
N.D.
-3.7
+0.0
N.D.
N.D.
gfp_control
GFP_control
N.D.
-3.5
-0.0
N.D.
N.D.
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
N.D.
-3.6
+0.1
N.D.
N.D.
drt_type_3
DRT_type_3
N.D.
-3.6
+0.1
N.D.
N.D.
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
-3.3
-0.2
N.D.
N.D.
phage
phage
N.D.
-3.7
+0.3
N.D.
N.D.
ppi
ppI
N.D.
-3.3
-0.1
N.D.
N.D.
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
N.D.
-3.6
+0.2
N.D.
N.D.
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