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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rpmH and rnpA are separated by 16 nucleotides
rnpA and yidD overlap by 37 nucleotides
yidD and yidC are separated by 2 nucleotides
yidC and trmE are separated by 105 nucleotides
b3703: rpmH - 50S ribosomal protein L34 (NCBI), at 3,882,359 to 3,882,499
rpmH
b3704: rnpA - ribonuclease P (NCBI), at 3,882,516 to 3,882,875
rnpA
b4557: yidD - hypothetical protein (NCBI), at 3,882,839 to 3,883,096
yidD
b3705: yidC - inner membrane protein translocase component YidC (NCBI), at 3,883,099 to 3,884,745
yidC
b3706: trmE - tRNA modification GTPase (NCBI), at 3,884,851 to 3,886,215
trmE
Group
Condition
b3703
b3704
b4557
b3705
b3706
rpmH
rnpA
yidD
yidC
trmE
sulfur source
M63 no sulfur + 5 mM sulfate
N.D.
N.D.
-0.2
N.D.
-4.3
sulfur source
M63 no sulfur + 5 mM L-cys
N.D.
N.D.
-0.1
N.D.
-4.4
control
PBS_cage_2_mouse_1_Cecum_40 hr
N.D.
N.D.
-0.8
N.D.
-3.7
control
PBS_cage_2_mouse_2_Cecum_40 hr
N.D.
N.D.
-0.2
N.D.
-4.3
stress
2,4-Dinitrophenol 2 mM
N.D.
N.D.
-0.3
N.D.
-4.1
stress
2,4-Dinitrophenol 2 mM
N.D.
N.D.
-0.2
N.D.
-4.2
sulfur source
M63 no sulfur + 5 mM sulfate
N.D.
N.D.
+0.3
N.D.
-4.3
nitrogen source
Putrescine (N)
N.D.
N.D.
+0.1
N.D.
-4.1
stress
toluene 2.5 vol%
N.D.
N.D.
-0.7
N.D.
-3.3
nitrogen source
Gly-DL-Asp (N)
N.D.
N.D.
-0.6
N.D.
-3.3
nitrogen source
Putrescine (N)
N.D.
N.D.
+0.0
N.D.
-3.9
phage
Phage_N4_cage_3_mice _Feces_22 hr
N.D.
N.D.
-0.0
N.D.
-3.8
micoeukaryotes
Tetrahymena sp., Mixed culture-1
N.D.
N.D.
-1.3
N.D.
-2.5
carbon source
D-Serine (C)
N.D.
N.D.
-0.1
N.D.
-3.7
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
-2.3
N.D.
-1.5
carbon source
Dodecanol (C)
N.D.
N.D.
-1.0
N.D.
-2.7
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
N.D.
N.D.
-0.1
N.D.
-3.6
sulfur source
M63 no sulfur + 5 mM L-cys
N.D.
N.D.
-0.1
N.D.
-3.6
carbon source
Dodecanol and Rhamnolipid (C)
N.D.
N.D.
+0.1
N.D.
-3.7
carbon source
Dodecanol and Rhamnolipid (C)
N.D.
N.D.
+0.2
N.D.
-3.7
phage
Phage_N4_cage_3_mice _Feces_40 hr
N.D.
N.D.
+0.3
N.D.
-3.7
nitrogen source
Glycine (N)
N.D.
N.D.
+0.3
N.D.
-3.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
N.D.
-4.0
N.D.
+0.8
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
N.D.
N.D.
+0.8
N.D.
-3.5
stress
Cobalt chloride 0.32 mM
N.D.
N.D.
+0.7
N.D.
-2.9
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
-3.3
N.D.
+2.2
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
-2.3
N.D.
+1.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
-2.6
N.D.
+3.1
stress
Benzalkonium Chloride 0.004 mg/ml
N.D.
N.D.
-2.9
N.D.
+4.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
-1.3
N.D.
+2.4
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