Fitness for 5 genes in Escherichia coli BW25113

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500 ntyiaC and bisC overlap by 32 nucleotidesbisC and yiaD are separated by 152 nucleotidesyiaD and yiaE are separated by 103 nucleotidesyiaE and yiaF are separated by 49 nucleotides b3550: yiaC - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,711,675 to 3,712,115 yiaC b3551: bisC - biotin sulfoxide reductase (RefSeq), at 3,712,084 to 3,714,417 bisC b3552: yiaD - predicted outer membrane lipoprotien (NCBI), at 3,714,570 to 3,715,229 yiaD b3553: yiaE - putative dehydrogenase (VIMSS), at 3,715,333 to 3,716,307 yiaE b3554: yiaF - orf, hypothetical protein (VIMSS), at 3,716,357 to 3,717,067 yiaF
Group Condition b3550 b3551 b3552 b3553 b3554
yiaC bisC yiaD yiaE yiaF
stress Bacitracin 1 mg/ml -0.1 -0.0 -0.0 +0.1 -3.5
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.0 -0.2 -0.1 +0.3 -2.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.2 +0.0 -0.2 -0.1 -2.2
stress Bacitracin 0.5 mg/ml +0.1 +0.0 -0.2 +0.0 -1.6
stress LB Aerobic with Ramoplanin 0.000125 mM -0.0 +0.0 -0.0 -0.0 -1.6
carbon source carbon source D-Mannose 5mM -0.2 +0.0 -0.2 -0.4 -0.6
carbon source carbon source D-Glucose 5mM -0.1 +0.2 -0.4 -0.6 -0.5
mzaabcde mzaABCDE -0.3 -0.0 -0.3 -0.2 -0.5
mzaabcde mzaABCDE -0.1 -0.2 -0.2 -0.2 -0.6
retron_ec_48_control retron_Ec_48_control -0.3 -0.0 -0.2 -0.3 -0.4
gfp_control GFP_control -0.7 -0.1 -0.0 -0.0 -0.5
stress pinene 5 vol% -0.4 -0.2 -0.1 -0.1 -0.4
carbon source carbon source D-Mannose 1mM -0.3 +0.1 -0.3 -0.3 -0.4
carbon source D-Serine (C) -0.0 +0.0 -0.4 -0.8 +0.0
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 +0.1 +0.1 +0.1 -1.5
carbon source carbon source D-Glucose 20mM +0.2 +0.0 -0.2 -0.3 -0.6
carbon source carbon source D-Mannose 20mM +0.2 +0.1 -0.3 -0.3 -0.5
phage Δdbn_10 +0.4 -0.0 -0.9 +0.4 -0.2
stress Benzalkonium Chloride 0.004 mg/ml -0.1 +0.4 -0.0 +0.2 -0.7
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 -0.4 -0.2 +0.3 -0.3
phage T7_phage 0.00015625 MOI TC4 -0.0 -0.2 -0.2 +0.5 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -0.1 -0.2 -0.0 +0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 +0.1 -0.1 -0.1 +0.6
motility outer cut, LB soft agar motility assay +0.9 -0.1 +0.0 -0.3 -0.2
phage T7_phage 0.0015625 MOI TC2 +0.3 +0.1 -0.2 -0.4 +0.5
stress Aluminum chloride 5 mM -0.4 -0.0 -0.1 +0.3 +0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -0.3 +0.5 +0.2 -0.0
stress Trimethoprim 0.00125 mM -0.3 +0.0 -0.1 +0.2 +0.7
motility inner cut, LB soft agar motility assay +0.3 +0.3 +0.3 -0.3 -0.1
stress Chlorite 1.25 mM +0.7 -0.1 +0.3 -0.0 +0.1
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