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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yiaC and bisC overlap by 32 nucleotides
bisC and yiaD are separated by 152 nucleotides
yiaD and yiaE are separated by 103 nucleotides
yiaE and yiaF are separated by 49 nucleotides
b3550: yiaC - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,711,675 to 3,712,115
yiaC
b3551: bisC - biotin sulfoxide reductase (RefSeq), at 3,712,084 to 3,714,417
bisC
b3552: yiaD - predicted outer membrane lipoprotien (NCBI), at 3,714,570 to 3,715,229
yiaD
b3553: yiaE - putative dehydrogenase (VIMSS), at 3,715,333 to 3,716,307
yiaE
b3554: yiaF - orf, hypothetical protein (VIMSS), at 3,716,357 to 3,717,067
yiaF
Group
Condition
b3550
b3551
b3552
b3553
b3554
yiaC
bisC
yiaD
yiaE
yiaF
stress
Bacitracin 1 mg/ml
-0.1
-0.0
-0.0
+0.1
-3.5
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.0
-0.2
-0.1
+0.3
-2.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.2
+0.0
-0.2
-0.1
-2.2
stress
Bacitracin 0.5 mg/ml
+0.1
+0.0
-0.2
+0.0
-1.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.0
+0.0
-0.0
-0.0
-1.6
carbon source
carbon source D-Mannose 5mM
-0.2
+0.0
-0.2
-0.4
-0.6
carbon source
carbon source D-Glucose 5mM
-0.1
+0.2
-0.4
-0.6
-0.5
mzaabcde
mzaABCDE
-0.3
-0.0
-0.3
-0.2
-0.5
mzaabcde
mzaABCDE
-0.1
-0.2
-0.2
-0.2
-0.6
retron_ec_48_control
retron_Ec_48_control
-0.3
-0.0
-0.2
-0.3
-0.4
gfp_control
GFP_control
-0.7
-0.1
-0.0
-0.0
-0.5
stress
pinene 5 vol%
-0.4
-0.2
-0.1
-0.1
-0.4
carbon source
carbon source D-Mannose 1mM
-0.3
+0.1
-0.3
-0.3
-0.4
carbon source
D-Serine (C)
-0.0
+0.0
-0.4
-0.8
+0.0
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.3
+0.1
+0.1
+0.1
-1.5
carbon source
carbon source D-Glucose 20mM
+0.2
+0.0
-0.2
-0.3
-0.6
carbon source
carbon source D-Mannose 20mM
+0.2
+0.1
-0.3
-0.3
-0.5
phage
Δdbn_10
+0.4
-0.0
-0.9
+0.4
-0.2
stress
Benzalkonium Chloride 0.004 mg/ml
-0.1
+0.4
-0.0
+0.2
-0.7
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.2
-0.4
-0.2
+0.3
-0.3
phage
T7_phage 0.00015625 MOI TC4
-0.0
-0.2
-0.2
+0.5
-0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.1
-0.2
-0.0
+0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.5
+0.1
-0.1
-0.1
+0.6
motility
outer cut, LB soft agar motility assay
+0.9
-0.1
+0.0
-0.3
-0.2
phage
T7_phage 0.0015625 MOI TC2
+0.3
+0.1
-0.2
-0.4
+0.5
stress
Aluminum chloride 5 mM
-0.4
-0.0
-0.1
+0.3
+0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
-0.3
+0.5
+0.2
-0.0
stress
Trimethoprim 0.00125 mM
-0.3
+0.0
-0.1
+0.2
+0.7
motility
inner cut, LB soft agar motility assay
+0.3
+0.3
+0.3
-0.3
-0.1
stress
Chlorite 1.25 mM
+0.7
-0.1
+0.3
-0.0
+0.1
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