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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhhI and yhhJ are separated by 164 nucleotides
yhhJ and rbbA overlap by 1 nucleotides
rbbA and yhiI overlap by 4 nucleotides
yhiI and yhiJ are separated by 365 nucleotides
b3484: yhhI - predicted transposase (NCBI), at 3,622,401 to 3,623,537
yhhI
b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826
yhhJ
b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561
rbbA
b3487: yhiI - predicted HlyD family secretion protein (NCBI), at 3,627,558 to 3,628,625
yhiI
b3488: yhiJ - hypothetical protein (NCBI), at 3,628,991 to 3,630,613
yhiJ
Group
Condition
b3484
b3485
b3486
b3487
b3488
yhhI
yhhJ
rbbA
yhiI
yhiJ
carbon source
carbon source D-Glucose 0.25mM
-5.0
-0.4
+0.1
-0.2
+0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.8
-0.4
-0.1
-0.8
-0.3
phage
T7_phage 0.0015625 MOI TC2
-2.5
-0.3
-0.1
+0.1
+0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.7
+0.0
+0.2
-0.2
-0.2
predation
6 hr rep1_HD100_10fold_dilution_grewON
-1.8
-0.0
-0.1
+0.3
-0.3
stress
LB Anaerobic with Ticarcillin 0.00003125 mM
-1.4
-0.1
-0.0
-0.0
-0.2
stress
Aluminum chloride 5 mM
-1.5
-0.2
-0.1
+0.1
-0.1
motility
inner cut, LB soft agar motility assay
-0.6
-0.6
+0.0
-0.2
-0.2
carbon source
carbon source D-Glucose 0.25mM
-1.7
-0.3
+0.4
-0.1
+0.2
carbon source
carbon source D-Mannose 20mM
-1.4
+0.2
+0.1
-0.0
-0.3
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-1.5
+0.1
-0.0
+0.1
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.3
+0.1
+0.1
-0.0
-0.1
stress
LB Anaerobic with Hygromycin B 0.00025 mM
-1.1
-0.1
+0.0
+0.3
-0.2
phage
Ste41
+0.3
-0.6
-0.4
-0.1
-0.4
stress
3-methyl-1-butanol 1.25 vol%
-0.8
+0.2
-0.1
-0.3
+0.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-1.4
-0.1
-0.1
+0.4
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.1
+0.1
+0.1
+0.3
-0.4
stress
LB Anaerobic with Mecillinam 0.00003125 mM
-0.9
-0.1
-0.2
+0.3
+0.2
phage
Lambda_ci1857
-0.7
+0.3
-0.2
+0.2
-0.2
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.9
+0.3
-0.1
-0.4
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.5
-0.4
+0.1
+0.5
-0.2
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.5
-0.3
-0.1
-0.6
+0.1
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.7
-0.1
-0.0
+0.5
+0.2
carbon source
carbon source D-Mannose 5mM
+0.7
-0.1
+0.1
-0.1
-0.6
carbon source
carbon source D-Mannose 5mM
+1.2
-0.3
-0.1
-1.0
+0.4
control
PBS_cage_2_mouse_1_Cecum_40 hr
+0.7
-0.2
+0.3
-0.4
+0.2
phage
Δdbn_10
+0.7
-0.5
-0.1
+0.2
+0.4
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+1.1
-0.1
-0.0
-0.3
+0.1
stress
octanol 0.08 vol%
+1.2
-0.2
+0.2
+0.2
+0.2
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+1.4
+0.3
+0.3
+0.2
+0.3
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