Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhhI and yhhJ are separated by 164 nucleotidesyhhJ and rbbA overlap by 1 nucleotidesrbbA and yhiI overlap by 4 nucleotidesyhiI and yhiJ are separated by 365 nucleotides b3484: yhhI - predicted transposase (NCBI), at 3,622,401 to 3,623,537 yhhI b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826 yhhJ b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561 rbbA b3487: yhiI - predicted HlyD family secretion protein (NCBI), at 3,627,558 to 3,628,625 yhiI b3488: yhiJ - hypothetical protein (NCBI), at 3,628,991 to 3,630,613 yhiJ
Group Condition b3484 b3485 b3486 b3487 b3488
yhhI yhhJ rbbA yhiI yhiJ
carbon source carbon source D-Glucose 0.25mM -5.0 -0.4 +0.1 -0.2 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.8 -0.4 -0.1 -0.8 -0.3
phage T7_phage 0.0015625 MOI TC2 -2.5 -0.3 -0.1 +0.1 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM -1.7 +0.0 +0.2 -0.2 -0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON -1.8 -0.0 -0.1 +0.3 -0.3
stress LB Anaerobic with Ticarcillin 0.00003125 mM -1.4 -0.1 -0.0 -0.0 -0.2
stress Aluminum chloride 5 mM -1.5 -0.2 -0.1 +0.1 -0.1
motility inner cut, LB soft agar motility assay -0.6 -0.6 +0.0 -0.2 -0.2
carbon source carbon source D-Glucose 0.25mM -1.7 -0.3 +0.4 -0.1 +0.2
carbon source carbon source D-Mannose 20mM -1.4 +0.2 +0.1 -0.0 -0.3
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -1.5 +0.1 -0.0 +0.1 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.3 +0.1 +0.1 -0.0 -0.1
stress LB Anaerobic with Hygromycin B 0.00025 mM -1.1 -0.1 +0.0 +0.3 -0.2
phage Ste41 +0.3 -0.6 -0.4 -0.1 -0.4
stress 3-methyl-1-butanol 1.25 vol% -0.8 +0.2 -0.1 -0.3 +0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -1.4 -0.1 -0.1 +0.4 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.1 +0.1 +0.1 +0.3 -0.4
stress LB Anaerobic with Mecillinam 0.00003125 mM -0.9 -0.1 -0.2 +0.3 +0.2
phage Lambda_ci1857 -0.7 +0.3 -0.2 +0.2 -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr -0.9 +0.3 -0.1 -0.4 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -0.4 +0.1 +0.5 -0.2
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.5 -0.3 -0.1 -0.6 +0.1
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.7 -0.1 -0.0 +0.5 +0.2
carbon source carbon source D-Mannose 5mM +0.7 -0.1 +0.1 -0.1 -0.6
carbon source carbon source D-Mannose 5mM +1.2 -0.3 -0.1 -1.0 +0.4
control PBS_cage_2_mouse_1_Cecum_40 hr +0.7 -0.2 +0.3 -0.4 +0.2
phage Δdbn_10 +0.7 -0.5 -0.1 +0.2 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +1.1 -0.1 -0.0 -0.3 +0.1
stress octanol 0.08 vol% +1.2 -0.2 +0.2 +0.2 +0.2
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +1.4 +0.3 +0.3 +0.2 +0.3
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