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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
malP and malT are separated by 611 nucleotides
malT and rtcA are separated by 42 nucleotides
rtcA and rtcB are separated by 3 nucleotides
rtcB and rtcR are separated by 188 nucleotides
b3417: malP - maltodextrin phosphorylase (NCBI), at 3,548,102 to 3,550,495
malP
b3418: malT - transcriptional regulator MalT (NCBI), at 3,551,107 to 3,553,812
malT
b4475: rtcA - RNA 3'-terminal-phosphate cyclase (NCBI), at 3,553,855 to 3,554,871
rtcA
b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101
rtcB
b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888
rtcR
Group
Condition
b3417
b3418
b4475
b3421
b3422
malP
malT
rtcA
rtcB
rtcR
carbon source
D-Maltose (C)
-1.7
-2.9
-0.1
-0.1
-0.0
carbon source
D-Maltose (C)
-1.8
-2.7
-0.1
+0.0
+0.1
stress
2,4-Dinitrophenol 2 mM
-0.1
-1.8
+0.0
-0.1
+0.0
stress
2,4-Dinitrophenol 2 mM
-0.2
-1.8
-0.0
+0.0
+0.1
phage
T7_phage 0.00015625 MOI TC4
-0.0
-0.2
-0.4
-0.2
-0.7
phage
T7_phage 0.0015625 MOI TC1
+0.1
-0.3
-0.3
-0.3
-0.6
stress
methylglyoxal 0.008 vol%
-1.1
+0.0
-0.2
-0.1
-0.0
stress
Cephalothin 0.01 mg/ml
-0.9
-0.2
-0.2
+0.3
-0.3
phage
T7_phage 0.0015625 MOI TC2
+0.1
-0.0
-0.1
-0.6
-0.6
stress
Carbenicillin 0.008 mg/ml
-0.8
+0.1
-0.1
-0.2
+0.0
stress
octanol 0.08 vol%
-0.3
-0.0
-0.6
+0.2
+0.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.7
+0.6
-0.2
+0.0
+0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.5
-0.4
-0.2
+0.0
-0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.4
+0.4
-0.2
+0.2
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.9
+0.9
+0.0
+0.1
-0.1
stress
Bacitracin 1 mg/ml
-0.5
+0.4
-0.2
+0.3
+0.3
stress
2-Furfuraldehyde 0.25 vol%
+0.6
-0.6
+0.2
+0.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.2
+1.0
+0.1
+0.0
+0.0
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.2
-0.1
+0.4
+0.5
+0.2
stress
Spectinomycin 0.0125 mg/ml
+1.4
+0.1
-0.0
-0.2
-0.0
motility
inner cut, LB soft agar motility assay
-0.0
+0.5
+0.3
+0.3
+0.1
motility
outer cut, LB soft agar motility assay
-0.8
+1.5
+0.3
-0.1
+0.4
stress
Cisplatin 0.1 mg/ml
+0.7
+0.2
+0.3
-0.0
+0.2
stress
Benzalkonium Chloride 0.004 mg/ml
+2.2
+0.7
+0.2
+0.0
-0.0
phage
Papa dilution 10-1 cacl2 maltose
+0.2
+1.0
+0.6
+0.6
+1.5
fr_100_e2
Fr_100_E2
-0.1
+4.8
-0.1
-0.1
+0.0
fr_1000_e3
Fr_1000_E3
+0.0
+6.2
-0.1
+0.1
-0.1
fr_1000_f3
Fr_1000_F3
-0.1
+6.6
-0.1
+0.2
+0.0
phage
Lambda_ci1857
-1.2
+7.8
+0.4
+0.4
-0.2
fr_100_f2
Fr_100_F2
+0.1
+7.7
-0.0
+0.1
-0.0
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