Fitness for 5 genes in Escherichia coli BW25113

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500 ntmalP and malT are separated by 611 nucleotidesmalT and rtcA are separated by 42 nucleotidesrtcA and rtcB are separated by 3 nucleotidesrtcB and rtcR are separated by 188 nucleotides b3417: malP - maltodextrin phosphorylase (NCBI), at 3,548,102 to 3,550,495 malP b3418: malT - transcriptional regulator MalT (NCBI), at 3,551,107 to 3,553,812 malT b4475: rtcA - RNA 3'-terminal-phosphate cyclase (NCBI), at 3,553,855 to 3,554,871 rtcA b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101 rtcB b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888 rtcR
Group Condition b3417 b3418 b4475 b3421 b3422
malP malT rtcA rtcB rtcR
carbon source D-Maltose (C) -1.7 -2.9 -0.1 -0.1 -0.0
carbon source D-Maltose (C) -1.8 -2.7 -0.1 +0.0 +0.1
stress 2,4-Dinitrophenol 2 mM -0.1 -1.8 +0.0 -0.1 +0.0
stress 2,4-Dinitrophenol 2 mM -0.2 -1.8 -0.0 +0.0 +0.1
phage T7_phage 0.00015625 MOI TC4 -0.0 -0.2 -0.4 -0.2 -0.7
phage T7_phage 0.0015625 MOI TC1 +0.1 -0.3 -0.3 -0.3 -0.6
stress methylglyoxal 0.008 vol% -1.1 +0.0 -0.2 -0.1 -0.0
stress Cephalothin 0.01 mg/ml -0.9 -0.2 -0.2 +0.3 -0.3
phage T7_phage 0.0015625 MOI TC2 +0.1 -0.0 -0.1 -0.6 -0.6
stress Carbenicillin 0.008 mg/ml -0.8 +0.1 -0.1 -0.2 +0.0
stress octanol 0.08 vol% -0.3 -0.0 -0.6 +0.2 +0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.7 +0.6 -0.2 +0.0 +0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.5 -0.4 -0.2 +0.0 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 +0.4 -0.2 +0.2 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.9 +0.9 +0.0 +0.1 -0.1
stress Bacitracin 1 mg/ml -0.5 +0.4 -0.2 +0.3 +0.3
stress 2-Furfuraldehyde 0.25 vol% +0.6 -0.6 +0.2 +0.2 +0.2
motility outer cut, LB soft agar motility assay -0.2 +1.0 +0.1 +0.0 +0.0
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -0.1 +0.4 +0.5 +0.2
stress Spectinomycin 0.0125 mg/ml +1.4 +0.1 -0.0 -0.2 -0.0
motility inner cut, LB soft agar motility assay -0.0 +0.5 +0.3 +0.3 +0.1
motility outer cut, LB soft agar motility assay -0.8 +1.5 +0.3 -0.1 +0.4
stress Cisplatin 0.1 mg/ml +0.7 +0.2 +0.3 -0.0 +0.2
stress Benzalkonium Chloride 0.004 mg/ml +2.2 +0.7 +0.2 +0.0 -0.0
phage Papa dilution 10-1 cacl2 maltose +0.2 +1.0 +0.6 +0.6 +1.5
fr_100_e2 Fr_100_E2 -0.1 +4.8 -0.1 -0.1 +0.0
fr_1000_e3 Fr_1000_E3 +0.0 +6.2 -0.1 +0.1 -0.1
fr_1000_f3 Fr_1000_F3 -0.1 +6.6 -0.1 +0.2 +0.0
phage Lambda_ci1857 -1.2 +7.8 +0.4 +0.4 -0.2
fr_100_f2 Fr_100_F2 +0.1 +7.7 -0.0 +0.1 -0.0
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