Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhgH and gntY are separated by 58 nucleotidesgntY and gntT are separated by 359 nucleotidesgntT and malQ are separated by 110 nucleotidesmalQ and malP are separated by 9 nucleotides b3413: yhgH - orf, hypothetical protein (VIMSS), at 3,542,904 to 3,543,587 yhgH b3414: gntY - predicted gluconate transport associated protein (NCBI), at 3,543,646 to 3,544,221 gntY b3415: gntT - gluconate transporter, high-affinity GNT I system (NCBI), at 3,544,581 to 3,545,897 gntT b3416: malQ - 4-alpha-glucanotransferase (amylomaltase) (NCBI), at 3,546,008 to 3,548,092 malQ b3417: malP - maltodextrin phosphorylase (NCBI), at 3,548,102 to 3,550,495 malP
Group Condition b3413 b3414 b3415 b3416 b3417
yhgH gntY gntT malQ malP
carbon source D-Maltose (C) +0.0 -0.7 -0.1 -5.3 -1.7
carbon source D-Maltose (C) -0.3 -0.5 -0.1 -4.7 -1.8
stress 2,4-Dinitrophenol 2 mM -0.1 -5.4 +0.0 -0.4 -0.1
carbon source Glycolic Acid (C) -0.1 -4.4 +0.1 -0.8 -0.3
carbon source Glycolic Acid (C) +0.2 -4.4 +0.1 -0.6 -0.2
carbon source acetate (C) -0.2 -4.5 +0.1 -0.3 -0.0
phage Lambda_ci1857 +0.3 -2.5 -0.2 -1.3 -1.2
carbon source Ying_AminoAcid20 rep B; time point 3 -0.2 -4.3 -0.1 -0.1 -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 -0.0 -4.4 -0.0 -0.2 -0.0
carbon source acetate (C) +0.1 -4.3 -0.0 -0.4 -0.1
stress 2,4-Dinitrophenol 2 mM +0.1 -4.1 +0.0 -0.4 -0.2
protonated/deuterated deuterated glucose in D2O -0.1 -4.4 +0.0 -0.1 +0.1
carbon source L-Malic (C) -0.1 -3.4 +0.1 -0.3 -0.3
protonated/deuterated deuterated glucose in D2O -0.1 -3.9 +0.1 -0.1 +0.1
stress 3-methyl-2-butenol 0.625 vol% +0.3 -3.7 +0.1 -0.1 +0.1
motility outer cut, LB soft agar motility assay -0.0 +2.1 -0.2 -1.4 -0.8
stress Spectinomycin 0.00625 mg/ml -0.2 +1.9 +0.0 -2.3 +0.7
stress Spectinomycin 0.0125 mg/ml -0.3 +2.9 -0.1 -2.5 +1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +3.5 +0.0 +0.5 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +3.9 -0.1 +0.8 -0.3
plasmid transformation vcDART vector transformation; agar assay +0.1 +4.4 -0.0 +0.8 +0.2
plasmid transformation vcDART vector transformation; agar assay +0.3 +4.5 -0.0 +0.8 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +4.5 -0.2 +1.2 +0.3
plasmid transformation mariner vector transformation; agar assay +0.3 +5.4 -0.1 +0.6 +0.2
plasmid transformation mariner vector transformation; agar assay +0.3 +5.3 +0.1 +0.6 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 +4.9 +0.0 +1.1 +0.1
plasmid transformation mariner vector transformation; agar assay +0.2 +5.7 -0.0 +0.7 +0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.7 +2.9 +0.3 +2.1 +2.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +6.8 -0.3 +0.1 +0.1
plasmid transformation vcDART vector transformation; agar assay +0.2 +5.7 +0.0 +0.8 +0.2
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