Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhfX and yhfY are separated by 83 nucleotidesyhfY and yhfZ are separated by 16 nucleotidesyhfZ and trpS are separated by 289 nucleotidestrpS and gph overlap by 8 nucleotides b3381: yhfX - predicted amino acid racemase (NCBI), at 3,507,835 to 3,508,998 yhfX b3382: yhfY - orf, hypothetical protein (VIMSS), at 3,509,082 to 3,509,444 yhfY b3383: yhfZ - hypothetical protein (NCBI), at 3,509,461 to 3,510,366 yhfZ b3384: trpS - tryptophanyl-tRNA synthetase (NCBI), at 3,510,656 to 3,511,660 trpS b3385: gph - phosphoglycolate phosphatase (NCBI), at 3,511,653 to 3,512,411 gph
Group Condition b3381 b3382 b3383 b3384 b3385
yhfX yhfY yhfZ trpS gph
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.9 -0.4 -1.7 N.D. +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 -0.3 -2.0 N.D. +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.9 -0.4 -1.4 N.D. +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.3 +0.2 -1.2 N.D. -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 -0.3 -0.9 N.D. +0.1
stress Cisplatin 0.1 mg/ml -0.6 +0.1 -0.9 N.D. -0.4
stress Cisplatin 0.1 mg/ml -0.7 +0.1 -1.1 N.D. -0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.8 -0.2 -0.6 N.D. -0.1
stress Cisplatin 0.1 mg/ml -0.6 -0.3 -0.9 N.D. +0.1
stress Cisplatin 0.1 mg/ml -1.0 +0.2 -0.8 N.D. -0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.1 +0.4 -0.9 N.D. -0.1
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.6 -0.4 -0.6 N.D. -0.0
stress Cisplatin 0.1 mg/ml -0.6 -0.0 -0.9 N.D. -0.0
carbon source Ying_Nucleobase14 rep C; time point 3 +0.1 -0.0 +0.0 N.D. -1.6
stress 2,4-Dinitrophenol 2 mM -0.3 -0.3 -0.5 N.D. -0.5
carbon source Ying_Nucleobase14 rep A; time point 3 -0.0 +0.1 -0.1 N.D. -1.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.7 +0.2 -0.8 N.D. -0.1
carbon source Ying_Nucleobase14 rep C; time point 2 -0.0 +0.2 -0.1 N.D. -1.4
carbon source Ying_Nucleobase14 rep B; time point 2 +0.0 +0.1 -0.0 N.D. -1.4
stress Cisplatin 0.05 mg/ml -0.8 +0.1 -0.7 N.D. +0.1
carbon source Ying_Nucleobase14 rep A; time point 2 +0.0 +0.1 +0.1 N.D. -1.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.7 +0.1 -0.7 N.D. +0.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.3 -1.0 -0.0 N.D. -0.3
carbon source Ying_Nucleobase14 rep B; time point 3 +0.2 +0.1 +0.3 N.D. -1.5
carbon source a-Ketoglutaric (C) +0.1 +0.3 +0.2 N.D. -1.1
carbon source a-Ketoglutaric (C) +0.1 +0.4 +0.2 N.D. -1.1
control PBS_cage_2_mouse_1_Cecum_40 hr -0.5 +0.8 -0.2 N.D. -0.2
carbon source carbon source D-Glucose 0.25mM -0.3 +0.8 +0.3 N.D. -0.5
stress limonene 5 vol% +0.7 +0.1 +1.3 N.D. +0.1
stress limonene 5 vol% +1.0 +0.2 +1.5 N.D. +0.2
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