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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhfM and yhfN are separated by 20 nucleotides
yhfN and frlC are separated by 49 nucleotides
frlC and frlD overlap by 4 nucleotides
frlD and yhfR are separated by 99 nucleotides
b3370: yhfM - putative amino acid/amine transport protein (VIMSS), at 3,497,932 to 3,499,269
yhfM
b3371: yhfN - putative transport protein (VIMSS), at 3,499,290 to 3,500,312
yhfN
b4474: frlC - predicted isomerase (NCBI), at 3,500,362 to 3,501,192
frlC
b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974
frlD
b3375: yhfR - putative transcriptional regulator (VIMSS), at 3,502,074 to 3,502,805
yhfR
Group
Condition
b3370
b3371
b4474
b3374
b3375
yhfM
yhfN
frlC
frlD
yhfR
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
-0.0
-0.2
-0.0
-0.0
-2.8
phage
phage
+0.1
-0.2
-0.1
-0.2
-2.5
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-0.0
-0.1
+0.1
+0.0
-2.9
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-0.0
-0.1
+0.0
-0.0
-2.5
stress
3-methyl-1-butanol 1.25 vol%
-0.1
-0.7
-0.3
-0.3
-1.1
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
-0.0
-0.0
+0.1
+0.0
-2.5
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
+0.0
-0.0
+0.0
-0.1
-2.4
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-0.1
+0.1
+0.0
+0.1
-2.4
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-0.0
+0.0
+0.1
-0.2
-2.2
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
-0.1
+0.1
+0.2
+0.2
-2.6
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.0
-0.3
+0.2
-0.4
-1.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.0
+0.0
-0.2
-1.9
phage
T7_phage 0.00015625 MOI TC4
-0.1
-0.4
-0.1
-0.0
-1.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
-0.2
-0.3
-0.3
-1.2
fishing
LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector
+0.0
+0.1
+0.0
+0.1
-1.8
carbon source
carbon source D-Mannose 1mM
+0.1
+0.0
-0.2
-0.8
-0.6
fishing
LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector
+0.0
-0.0
+0.0
+0.1
-1.5
lb anaerobic no stress control
LB anaerobic no stress control
+0.0
-0.2
+0.1
-0.0
-1.3
carbon source
Glycolic Acid (C)
+0.0
+0.3
+0.1
-0.1
-1.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
-0.6
-0.0
+0.0
-0.8
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.2
-0.3
+0.4
-0.4
-0.6
stress
Benzalkonium Chloride 0.004 mg/ml
+0.6
+0.5
-0.2
-0.2
+0.2
stress
LB Anaerobic with Azlocillin 0.00003125 mM
-0.1
-0.3
+0.0
+0.1
+1.3
mixed community
LB 0.1x with Mixed culture; Trichoderma harzianum M10
-0.1
-0.1
-0.0
+0.1
+1.3
stress
LB Anaerobic with Cefoxitin 0.0000015625 mM
+0.0
+0.1
-0.1
-0.1
+1.3
stress
LB Anaerobic with Ticarcillin 0.00003125 mM
-0.0
-0.1
-0.1
-0.1
+1.6
stress
LB Anaerobic with Penicillin g 0.0000625 mM
-0.1
+0.1
+0.1
-0.2
+1.4
stress
LB Anaerobic with Penicillin g 0.0000625 mM
-0.1
+0.2
+0.0
-0.0
+1.4
fr_1000_f3
Fr_1000_F3
+0.1
-0.1
+0.0
+0.1
+1.3
stress
LB Anaerobic with Amoxicillin 0.00000390625 mM
-0.0
+0.2
+0.1
+0.0
+1.5
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