Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhfM and yhfN are separated by 20 nucleotidesyhfN and frlC are separated by 49 nucleotidesfrlC and frlD overlap by 4 nucleotidesfrlD and yhfR are separated by 99 nucleotides b3370: yhfM - putative amino acid/amine transport protein (VIMSS), at 3,497,932 to 3,499,269 yhfM b3371: yhfN - putative transport protein (VIMSS), at 3,499,290 to 3,500,312 yhfN b4474: frlC - predicted isomerase (NCBI), at 3,500,362 to 3,501,192 frlC b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974 frlD b3375: yhfR - putative transcriptional regulator (VIMSS), at 3,502,074 to 3,502,805 yhfR
Group Condition b3370 b3371 b4474 b3374 b3375
yhfM yhfN frlC frlD yhfR
dart Outgrowth from conjugation with pRH22 in LB+Gent50 -0.0 -0.2 -0.0 -0.0 -2.8
phage phage +0.1 -0.2 -0.1 -0.2 -2.5
dart Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 -0.0 -0.1 +0.1 +0.0 -2.9
dart Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 -0.0 -0.1 +0.0 -0.0 -2.5
stress 3-methyl-1-butanol 1.25 vol% -0.1 -0.7 -0.3 -0.3 -1.1
dart Outgrowth from conjugation with pRH23 in LB+Gent50 -0.0 -0.0 +0.1 +0.0 -2.5
dart Outgrowth from conjugation with pRH23 in LB+Gent50 +0.0 -0.0 +0.0 -0.1 -2.4
dart Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 -0.1 +0.1 +0.0 +0.1 -2.4
dart Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 -0.0 +0.0 +0.1 -0.2 -2.2
dart Outgrowth from conjugation with pRH22 in LB+Gent50 -0.1 +0.1 +0.2 +0.2 -2.6
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.0 -0.3 +0.2 -0.4 -1.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.0 +0.0 -0.2 -1.9
phage T7_phage 0.00015625 MOI TC4 -0.1 -0.4 -0.1 -0.0 -1.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.2 -0.3 -0.3 -1.2
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector +0.0 +0.1 +0.0 +0.1 -1.8
carbon source carbon source D-Mannose 1mM +0.1 +0.0 -0.2 -0.8 -0.6
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector +0.0 -0.0 +0.0 +0.1 -1.5
lb anaerobic no stress control LB anaerobic no stress control +0.0 -0.2 +0.1 -0.0 -1.3
carbon source Glycolic Acid (C) +0.0 +0.3 +0.1 -0.1 -1.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -0.6 -0.0 +0.0 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 -0.3 +0.4 -0.4 -0.6
stress Benzalkonium Chloride 0.004 mg/ml +0.6 +0.5 -0.2 -0.2 +0.2
stress LB Anaerobic with Azlocillin 0.00003125 mM -0.1 -0.3 +0.0 +0.1 +1.3
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 -0.1 -0.1 -0.0 +0.1 +1.3
stress LB Anaerobic with Cefoxitin 0.0000015625 mM +0.0 +0.1 -0.1 -0.1 +1.3
stress LB Anaerobic with Ticarcillin 0.00003125 mM -0.0 -0.1 -0.1 -0.1 +1.6
stress LB Anaerobic with Penicillin g 0.0000625 mM -0.1 +0.1 +0.1 -0.2 +1.4
stress LB Anaerobic with Penicillin g 0.0000625 mM -0.1 +0.2 +0.0 -0.0 +1.4
fr_1000_f3 Fr_1000_F3 +0.1 -0.1 +0.0 +0.1 +1.3
stress LB Anaerobic with Amoxicillin 0.00000390625 mM -0.0 +0.2 +0.1 +0.0 +1.5
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