Fitness for 5 genes in Escherichia coli BW25113

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500 nttrkA and mscL are separated by 129 nucleotidesmscL and yhdL overlap by 4 nucleotidesyhdL and zntR are separated by 55 nucleotideszntR and yhdN are separated by 10 nucleotides b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916 trkA b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456 mscL b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671 yhdL b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152 zntR b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531 yhdN
Group Condition b3290 b3291 b4550 b3292 b3293
trkA mscL yhdL zntR yhdN
carbon source acetate (C) -2.6 -0.1 +0.1 -2.3 +0.1
carbon source acetate (C) -2.5 +0.1 +0.2 -3.1 +0.4
nitrogen source Putrescine (N) -2.8 -0.0 +0.1 -2.3 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.7 -0.2 -0.2 -2.3 -0.2
stress 3-methyl-2-butenol 1.25 vol% -3.1 +0.2 +0.2 -1.4 -0.2
stress 3-methyl-2-butenol 1.25 vol% -2.6 -0.1 +0.0 -1.1 -0.5
phage Phage_N4_cage_4_mice _Feces_40 hr -3.8 +0.1 +0.1 -0.4 -0.1
carbon source D-Sorbitol (C) -2.3 +0.1 +0.1 -2.0 +0.2
nitrogen source Putrescine (N) -2.6 +0.0 +0.4 -2.1 +0.4
carbon source L-Malic (C) -2.7 +0.0 +0.1 -1.8 +0.6
carbon source succinate (C) -2.7 -0.0 +0.2 -1.4 +0.2
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -1.2 -0.2 +0.4 -2.8 +0.3
carbon source Glycolic Acid (C) -2.7 +0.0 +0.3 -1.7 +0.6
carbon source L-Malic (C) -2.4 +0.1 +0.3 -1.8 +0.7
carbon source carbon source D-Glucose 0.25mM -1.7 +0.2 -1.3 -0.9 +0.6
carbon source D-Fructose (C) -2.6 +0.2 +0.1 -1.1 +0.3
carbon source a-Ketoglutaric (C) -2.4 +0.0 +0.3 -1.7 +0.7
carbon source Glycolic Acid (C) -2.6 +0.2 +0.3 -1.8 +0.8
carbon source a-Ketoglutaric (C) -2.2 +0.2 +0.3 -1.8 +0.5
carbon source D-Sorbitol (C) -2.5 +0.1 +0.3 -1.2 +0.4
motility outer cut, LB soft agar motility assay -3.4 -0.1 +0.1 +0.6 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.1 -0.5 -1.0 -0.9 +1.1
stress Doxycycline hyclate 0.001 mg/ml +3.3 -0.2 -1.2 -0.2 -0.7
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.4 +0.1 +2.1 -2.4 +1.0
stress Tetracycline 0.0008 mg/ml +2.7 -0.1 -1.4 +0.2 -0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.3 -0.3 +2.0 -2.1 +1.6
stress Cephalothin 0.01 mg/ml +3.5 -0.2 -0.0 +0.1 -1.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.6 -0.0 +2.4 -2.0 +1.9
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.8 +0.8 +1.9 -1.5 +1.5
stress Nalidixic 0.006 mg/ml +5.4 +0.0 +0.2 -0.1 -0.1
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