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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
trkA and mscL are separated by 129 nucleotides
mscL and yhdL overlap by 4 nucleotides
yhdL and zntR are separated by 55 nucleotides
zntR and yhdN are separated by 10 nucleotides
b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916
trkA
b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456
mscL
b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671
yhdL
b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152
zntR
b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531
yhdN
Group
Condition
b3290
b3291
b4550
b3292
b3293
trkA
mscL
yhdL
zntR
yhdN
carbon source
acetate (C)
-2.6
-0.1
+0.1
-2.3
+0.1
carbon source
acetate (C)
-2.5
+0.1
+0.2
-3.1
+0.4
nitrogen source
Putrescine (N)
-2.8
-0.0
+0.1
-2.3
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-1.7
-0.2
-0.2
-2.3
-0.2
stress
3-methyl-2-butenol 1.25 vol%
-3.1
+0.2
+0.2
-1.4
-0.2
stress
3-methyl-2-butenol 1.25 vol%
-2.6
-0.1
+0.0
-1.1
-0.5
phage
Phage_N4_cage_4_mice _Feces_40 hr
-3.8
+0.1
+0.1
-0.4
-0.1
carbon source
D-Sorbitol (C)
-2.3
+0.1
+0.1
-2.0
+0.2
nitrogen source
Putrescine (N)
-2.6
+0.0
+0.4
-2.1
+0.4
carbon source
L-Malic (C)
-2.7
+0.0
+0.1
-1.8
+0.6
carbon source
succinate (C)
-2.7
-0.0
+0.2
-1.4
+0.2
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-1.2
-0.2
+0.4
-2.8
+0.3
carbon source
Glycolic Acid (C)
-2.7
+0.0
+0.3
-1.7
+0.6
carbon source
L-Malic (C)
-2.4
+0.1
+0.3
-1.8
+0.7
carbon source
carbon source D-Glucose 0.25mM
-1.7
+0.2
-1.3
-0.9
+0.6
carbon source
D-Fructose (C)
-2.6
+0.2
+0.1
-1.1
+0.3
carbon source
a-Ketoglutaric (C)
-2.4
+0.0
+0.3
-1.7
+0.7
carbon source
Glycolic Acid (C)
-2.6
+0.2
+0.3
-1.8
+0.8
carbon source
a-Ketoglutaric (C)
-2.2
+0.2
+0.3
-1.8
+0.5
carbon source
D-Sorbitol (C)
-2.5
+0.1
+0.3
-1.2
+0.4
motility
outer cut, LB soft agar motility assay
-3.4
-0.1
+0.1
+0.6
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.1
-0.5
-1.0
-0.9
+1.1
stress
Doxycycline hyclate 0.001 mg/ml
+3.3
-0.2
-1.2
-0.2
-0.7
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.4
+0.1
+2.1
-2.4
+1.0
stress
Tetracycline 0.0008 mg/ml
+2.7
-0.1
-1.4
+0.2
-0.1
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.3
-0.3
+2.0
-2.1
+1.6
stress
Cephalothin 0.01 mg/ml
+3.5
-0.2
-0.0
+0.1
-1.2
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.6
-0.0
+2.4
-2.0
+1.9
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.8
+0.8
+1.9
-1.5
+1.5
stress
Nalidixic 0.006 mg/ml
+5.4
+0.0
+0.2
-0.1
-0.1
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