Fitness for 5 genes in Escherichia coli BW25113

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500 ntaroE and yrdC are separated by 4 nucleotidesyrdC and yrdD are separated by 4 nucleotidesyrdD and smg are separated by 28 nucleotidessmg and smf overlap by 29 nucleotides b3281: aroE - dehydroshikimate reductase, NAD(P)-binding (NCBI), at 3,428,042 to 3,428,860 aroE b3282: yrdC - predicted ribosome maturation factor (NCBI), at 3,428,865 to 3,429,437 yrdC b3283: yrdD - predicted DNA topoisomerase (NCBI), at 3,429,442 to 3,429,984 yrdD b3284: smg - hypothetical protein (NCBI), at 3,430,013 to 3,430,486 smg b4473: smf - hypothetical protein (NCBI), at 3,430,458 to 3,431,582 smf
Group Condition b3281 b3282 b3283 b3284 b4473
aroE yrdC yrdD smg smf
carbon source Ying_sugar7 rep B; time point 2 -8.8 N.D. +0.1 -0.0 -0.1
carbon source Ying_OrganicAcid7 rep B; time point 3 -8.5 N.D. +0.1 -0.1 -0.2
carbon source Ying_Nucleobase14 rep B; time point 3 -8.4 N.D. -0.1 -0.1 -0.1
carbon source Ying_sugar7 rep C; time point 2 -8.7 N.D. +0.1 -0.1 -0.1
carbon source Ying_Nucleobase14 rep C; time point 3 -8.4 N.D. -0.0 -0.1 -0.2
carbon source Ying_sugar7 rep B; time point 3 -8.6 N.D. +0.2 -0.1 -0.0
carbon source Ying_sugar7 rep A; time point 2 -8.5 N.D. +0.2 -0.1 -0.1
carbon source Ying_sugar7 rep A; time point 3 -8.6 N.D. +0.2 -0.1 -0.1
carbon source Ying_OrganicAcid7 rep C; time point 3 -8.3 N.D. +0.0 -0.1 +0.0
carbon source Ying_sugar7 rep C; time point 3 -8.5 N.D. +0.3 -0.3 +0.1
carbon source Ying_OrganicAcid7 rep A; time point 3 -8.3 N.D. +0.3 -0.1 -0.1
carbon source Ying_Nucleobase14 rep A; time point 3 -8.2 N.D. +0.0 -0.1 +0.0
carbon source Ying_Nucleobase14 rep B; time point 2 -7.7 N.D. -0.0 -0.1 +0.0
carbon source carbon source D-Mannose 1mM -6.8 N.D. +0.0 -0.9 -0.1
carbon source Ying_Nucleobase14 rep A; time point 2 -7.8 N.D. +0.0 +0.0 +0.0
carbon source carbon source D-Glucose 1mM -7.4 N.D. -0.0 -0.2 -0.1
carbon source Ying_Nucleobase14 rep C; time point 2 -7.4 N.D. +0.1 +0.1 -0.0
carbon source carbon source D-Glucose 1mM -6.8 N.D. +0.0 -0.3 -0.1
carbon source Ying_OrganicAcid7 rep B; time point 2 -6.9 N.D. +0.0 -0.2 -0.1
carbon source carbon source D-Glucose 0.25mM -6.0 N.D. -0.3 -0.5 -0.2
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin -7.0 N.D. +0.2 -0.2 +0.0
carbon source Ying_OrganicAcid7 rep C; time point 2 -6.7 N.D. +0.2 -0.3 -0.1
carbon source carbon source D-Glucose 0.5mM -6.3 N.D. -0.2 -0.4 -0.0
carbon source carbon source D-Mannose 1mM -6.6 N.D. +0.1 -0.3 -0.0
protonated/deuterated protonated control -6.7 N.D. +0.1 -0.2 +0.1
carbon source Ying_OrganicAcid7 rep A; time point 2 -6.8 N.D. +0.3 -0.1 -0.1
nutrient M9 Glucose 0.2%, Thauer's vit mix -6.6 N.D. +0.1 -0.1 +0.0
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -6.7 N.D. +0.1 -0.1 +0.1
carbon source a-Ketoglutaric (C) -5.2 N.D. +0.3 -1.3 +0.0
carbon source a-Ketoglutaric (C) -5.4 N.D. +0.6 -1.2 +0.1
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