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Cofit
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhaB and yhaC are separated by 21 nucleotides
yhaC and rnpB are separated by 613 nucleotides
rnpB and garK are separated by 32 nucleotides
garK and garR are separated by 96 nucleotides
b3120: yhaB - orf, hypothetical protein (VIMSS), at 3,265,876 to 3,266,415
yhaB
b3121: yhaC - hypothetical protein (NCBI), at 3,266,437 to 3,267,624
yhaC
b3123: rnpB - RNase P RNA component precursor RnpB (NCBI), at 3,268,238 to 3,268,614
rnpB
b3124: garK - glycerate kinase I (RefSeq), at 3,268,647 to 3,269,792
garK
b3125: garR - tartronate semialdehyde reductase (RefSeq), at 3,269,889 to 3,270,773
garR
Group
Condition
b3120
b3121
b3123
b3124
b3125
yhaB
yhaC
rnpB
garK
garR
carbon source
Glycolic Acid (C)
-0.6
-2.2
N.D.
-4.1
-1.3
carbon source
D-Glucuronic Acid (C)
-1.1
-1.0
N.D.
-4.9
-0.4
carbon source
Glycolic Acid (C)
-0.9
-0.8
N.D.
-4.5
-0.8
carbon source
D-Galacturonic Acid (C)
-0.8
-2.0
N.D.
-3.5
-0.6
carbon source
D-Galacturonic Acid (C)
-0.2
-1.8
N.D.
-3.4
-0.6
carbon source
D-Glucuronic Acid (C)
-0.4
-0.8
N.D.
-3.8
-0.5
carbon source
D-Galacturonic Acid (C) M9
-0.6
-0.6
N.D.
-3.6
-0.4
motility
inner cut, LB soft agar motility assay
-0.8
-4.3
N.D.
-0.3
+0.3
motility
inner cut, LB soft agar motility assay
-1.2
-3.7
N.D.
-0.5
+0.4
motility
outer cut, LB soft agar motility assay
-1.0
-3.4
N.D.
+0.0
-0.6
motility
outer cut, LB soft agar motility assay
-2.6
-2.7
N.D.
-0.5
+0.7
carbon source
D-Galacturonic Acid (C) MOPS
-0.4
-0.4
N.D.
-3.3
-0.5
motility
inner cut, LB soft agar motility assay
-1.4
-3.1
N.D.
-0.2
+0.3
motility
inner cut, LB soft agar motility assay
-1.2
-2.8
N.D.
-0.1
+0.0
carbon source
D-Ribose (C)
-0.2
-2.1
N.D.
-1.7
-0.1
carbon source
D-Serine (C)
-0.4
-0.4
N.D.
-3.0
-0.1
M9 Glucose 2% aerobic
-0.9
-2.4
N.D.
-0.2
-0.1
M9 Glucose 2%, 20 mM NO3- anaerobic
-0.9
-2.5
N.D.
-0.2
-0.0
protonated/deuterated
protonated glucose in D2O
-0.4
-2.4
N.D.
-0.6
-0.1
M9 Glucose 2% anaerobic
-1.1
-2.0
N.D.
-0.1
+0.0
stress
Cisplatin 0.1 mg/ml
-1.2
-2.2
N.D.
+0.1
+0.2
carbon source
D-Serine (C)
-0.5
+0.3
N.D.
-2.5
-0.3
stress
D-Cycloserine 0.1 mg/ml
-0.9
-2.1
N.D.
+0.2
-0.1
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.9
+1.3
N.D.
-1.4
-0.4
phage
T7_phage 0.0015625 MOI TC2
+0.7
+2.0
N.D.
-0.5
-0.2
control
PBS_cage_1_mice _Feces_16 hr
+1.4
+1.6
N.D.
-0.6
+0.4
stress
M9 with Methotrexate 1.98 mM 24 hr
+1.0
+2.2
N.D.
+0.2
-0.2
phage
R2_N4_T2_phage10
+2.3
+0.7
N.D.
-0.1
+0.4
phage
N4_phage 0.009375 MOI TC2
+1.5
+2.8
N.D.
-0.3
-0.0
phage
Papa dilution 10-1 cacl2 maltose
+2.0
+3.3
N.D.
+0.7
+0.1
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