Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhaB and yhaC are separated by 21 nucleotidesyhaC and rnpB are separated by 613 nucleotidesrnpB and garK are separated by 32 nucleotidesgarK and garR are separated by 96 nucleotides b3120: yhaB - orf, hypothetical protein (VIMSS), at 3,265,876 to 3,266,415 yhaB b3121: yhaC - hypothetical protein (NCBI), at 3,266,437 to 3,267,624 yhaC b3123: rnpB - RNase P RNA component precursor RnpB (NCBI), at 3,268,238 to 3,268,614 rnpB b3124: garK - glycerate kinase I (RefSeq), at 3,268,647 to 3,269,792 garK b3125: garR - tartronate semialdehyde reductase (RefSeq), at 3,269,889 to 3,270,773 garR
Group Condition b3120 b3121 b3123 b3124 b3125
yhaB yhaC rnpB garK garR
carbon source Glycolic Acid (C) -0.6 -2.2 N.D. -4.1 -1.3
carbon source D-Glucuronic Acid (C) -1.1 -1.0 N.D. -4.9 -0.4
carbon source Glycolic Acid (C) -0.9 -0.8 N.D. -4.5 -0.8
carbon source D-Galacturonic Acid (C) -0.8 -2.0 N.D. -3.5 -0.6
carbon source D-Galacturonic Acid (C) -0.2 -1.8 N.D. -3.4 -0.6
carbon source D-Glucuronic Acid (C) -0.4 -0.8 N.D. -3.8 -0.5
carbon source D-Galacturonic Acid (C) M9 -0.6 -0.6 N.D. -3.6 -0.4
motility inner cut, LB soft agar motility assay -0.8 -4.3 N.D. -0.3 +0.3
motility inner cut, LB soft agar motility assay -1.2 -3.7 N.D. -0.5 +0.4
motility outer cut, LB soft agar motility assay -1.0 -3.4 N.D. +0.0 -0.6
motility outer cut, LB soft agar motility assay -2.6 -2.7 N.D. -0.5 +0.7
carbon source D-Galacturonic Acid (C) MOPS -0.4 -0.4 N.D. -3.3 -0.5
motility inner cut, LB soft agar motility assay -1.4 -3.1 N.D. -0.2 +0.3
motility inner cut, LB soft agar motility assay -1.2 -2.8 N.D. -0.1 +0.0
carbon source D-Ribose (C) -0.2 -2.1 N.D. -1.7 -0.1
carbon source D-Serine (C) -0.4 -0.4 N.D. -3.0 -0.1
M9 Glucose 2% aerobic -0.9 -2.4 N.D. -0.2 -0.1
M9 Glucose 2%, 20 mM NO3- anaerobic -0.9 -2.5 N.D. -0.2 -0.0
protonated/deuterated protonated glucose in D2O -0.4 -2.4 N.D. -0.6 -0.1
M9 Glucose 2% anaerobic -1.1 -2.0 N.D. -0.1 +0.0
stress Cisplatin 0.1 mg/ml -1.2 -2.2 N.D. +0.1 +0.2
carbon source D-Serine (C) -0.5 +0.3 N.D. -2.5 -0.3
stress D-Cycloserine 0.1 mg/ml -0.9 -2.1 N.D. +0.2 -0.1
control PBS_cage_1_mouse_1_Cecum_40 hr -0.9 +1.3 N.D. -1.4 -0.4
phage T7_phage 0.0015625 MOI TC2 +0.7 +2.0 N.D. -0.5 -0.2
control PBS_cage_1_mice _Feces_16 hr +1.4 +1.6 N.D. -0.6 +0.4
stress M9 with Methotrexate 1.98 mM 24 hr +1.0 +2.2 N.D. +0.2 -0.2
phage R2_N4_T2_phage10 +2.3 +0.7 N.D. -0.1 +0.4
phage N4_phage 0.009375 MOI TC2 +1.5 +2.8 N.D. -0.3 -0.0
phage Papa dilution 10-1 cacl2 maltose +2.0 +3.3 N.D. +0.7 +0.1
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