Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhaL and yhaM are separated by 133 nucleotidesyhaM and yhaO are separated by 27 nucleotidesyhaO and tdcG are separated by 274 nucleotidestdcG and yhaR are separated by 71 nucleotides b3107: yhaL - orf, hypothetical protein (VIMSS), at 3,253,065 to 3,253,229 yhaL b4470: yhaM - hypothetical protein (NCBI), at 3,253,363 to 3,254,673 yhaM b3110: yhaO - predicted transporter (RefSeq), at 3,254,701 to 3,256,032 yhaO b4471: tdcG - L-serine dehydratase 3 (NCBI), at 3,256,307 to 3,257,671 tdcG b3113: yhaR - orf, hypothetical protein (VIMSS), at 3,257,743 to 3,258,132 yhaR
Group Condition b3107 b4470 b3110 b4471 b3113
yhaL yhaM yhaO tdcG yhaR
carbon source carbon source D-Glucose 0.25mM -5.9 +0.2 +0.2 -0.2 -0.4
stress octanol 0.08 vol% -5.3 -0.1 -0.0 -0.2 +0.4
phage Ste41 -4.1 -0.1 +0.2 -0.3 +0.0
motility outer cut, LB soft agar motility assay -3.3 -1.0 +0.0 +0.2 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -4.6 +0.2 -0.2 +0.1 +0.8
phage N4_phage 0.09375 MOI TC1 -2.5 +0.0 -0.2 -0.2 +0.0
protonated/deuterated deuterated glucose in H2O -3.5 -0.1 +0.1 +0.2 +0.6
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -1.7 -0.6 +0.4 -0.3 +0.2
motility outer cut, LB soft agar motility assay -1.2 -0.4 +0.0 +0.1 -0.4
stress 3-methyl-1-butanol 2.5 vol% -1.9 +0.0 +0.1 -0.1 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.4 -0.4 -0.2 +0.1 +0.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -1.9 +0.2 -0.0 +0.1 -0.0
control PBS_cage_2_mouse_2_Cecum_40 hr -1.4 -0.3 +0.2 +0.3 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.8 +0.3 -0.0 -0.2 +0.3
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None -1.7 +0.1 -0.2 -0.2 +0.5
carbon source carbon source D-Glucose 5mM -2.2 +0.3 +0.3 -0.1 +0.3
motility outer cut, LB soft agar motility assay -0.7 -0.6 +0.3 +0.3 -0.5
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -2.0 -0.1 +0.1 +0.0 +0.8
phage T7_phage 0.00015625 MOI TC4 -1.1 +0.3 -0.1 -0.6 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.1 -0.9 +0.2 +0.3 +0.6
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.7 -0.4 +0.1 -0.4 -0.8
gfp_control GFP_control -1.4 -0.2 +0.3 +0.3 +0.1
carbon source carbon source D-Mannose 0.5mM -1.5 +0.1 +0.1 +0.2 +0.3
stress 3-methyl-1-butanol 1.25 vol% -1.2 +0.2 +0.2 +0.1 +0.5
phage Δdbn_10 +1.4 -0.3 +0.1 -0.1 -1.4
carbon source carbon source D-Glucose 20mM +1.4 -0.4 +0.0 +0.3 -0.1
control PBS_cage_1_mouse_1_Cecum_40 hr +2.3 -0.6 +0.0 +0.5 -0.5
motility inner cut, LB soft agar motility assay +1.3 -0.3 +0.1 +0.1 +0.6
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +2.8 -0.2 -0.0 -0.2 -0.3
stress Chlorite 1.25 mM -0.8 +0.4 -0.3 +3.2 +2.8
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