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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhaL and yhaM are separated by 133 nucleotides
yhaM and yhaO are separated by 27 nucleotides
yhaO and tdcG are separated by 274 nucleotides
tdcG and yhaR are separated by 71 nucleotides
b3107: yhaL - orf, hypothetical protein (VIMSS), at 3,253,065 to 3,253,229
yhaL
b4470: yhaM - hypothetical protein (NCBI), at 3,253,363 to 3,254,673
yhaM
b3110: yhaO - predicted transporter (RefSeq), at 3,254,701 to 3,256,032
yhaO
b4471: tdcG - L-serine dehydratase 3 (NCBI), at 3,256,307 to 3,257,671
tdcG
b3113: yhaR - orf, hypothetical protein (VIMSS), at 3,257,743 to 3,258,132
yhaR
Group
Condition
b3107
b4470
b3110
b4471
b3113
yhaL
yhaM
yhaO
tdcG
yhaR
carbon source
carbon source D-Glucose 0.25mM
-5.9
+0.2
+0.2
-0.2
-0.4
stress
octanol 0.08 vol%
-5.3
-0.1
-0.0
-0.2
+0.4
phage
Ste41
-4.1
-0.1
+0.2
-0.3
+0.0
motility
outer cut, LB soft agar motility assay
-3.3
-1.0
+0.0
+0.2
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-4.6
+0.2
-0.2
+0.1
+0.8
phage
N4_phage 0.09375 MOI TC1
-2.5
+0.0
-0.2
-0.2
+0.0
protonated/deuterated
deuterated glucose in H2O
-3.5
-0.1
+0.1
+0.2
+0.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-1.7
-0.6
+0.4
-0.3
+0.2
motility
outer cut, LB soft agar motility assay
-1.2
-0.4
+0.0
+0.1
-0.4
stress
3-methyl-1-butanol 2.5 vol%
-1.9
+0.0
+0.1
-0.1
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.4
-0.4
-0.2
+0.1
+0.2
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-1.9
+0.2
-0.0
+0.1
-0.0
control
PBS_cage_2_mouse_2_Cecum_40 hr
-1.4
-0.3
+0.2
+0.3
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.8
+0.3
-0.0
-0.2
+0.3
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None
-1.7
+0.1
-0.2
-0.2
+0.5
carbon source
carbon source D-Glucose 5mM
-2.2
+0.3
+0.3
-0.1
+0.3
motility
outer cut, LB soft agar motility assay
-0.7
-0.6
+0.3
+0.3
-0.5
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-2.0
-0.1
+0.1
+0.0
+0.8
phage
T7_phage 0.00015625 MOI TC4
-1.1
+0.3
-0.1
-0.6
+0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-1.1
-0.9
+0.2
+0.3
+0.6
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
+0.7
-0.4
+0.1
-0.4
-0.8
gfp_control
GFP_control
-1.4
-0.2
+0.3
+0.3
+0.1
carbon source
carbon source D-Mannose 0.5mM
-1.5
+0.1
+0.1
+0.2
+0.3
stress
3-methyl-1-butanol 1.25 vol%
-1.2
+0.2
+0.2
+0.1
+0.5
phage
Δdbn_10
+1.4
-0.3
+0.1
-0.1
-1.4
carbon source
carbon source D-Glucose 20mM
+1.4
-0.4
+0.0
+0.3
-0.1
control
PBS_cage_1_mouse_1_Cecum_40 hr
+2.3
-0.6
+0.0
+0.5
-0.5
motility
inner cut, LB soft agar motility assay
+1.3
-0.3
+0.1
+0.1
+0.6
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+2.8
-0.2
-0.0
-0.2
-0.3
stress
Chlorite 1.25 mM
-0.8
+0.4
-0.3
+3.2
+2.8
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