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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yhaK and yhaL are separated by 22 nucleotides
yhaL and yhaM are separated by 133 nucleotides
yhaM and yhaO are separated by 27 nucleotides
yhaO and tdcG are separated by 274 nucleotides
b3106: yhaK - predicted pirin-related protein (NCBI), at 3,252,341 to 3,253,042
yhaK
b3107: yhaL - orf, hypothetical protein (VIMSS), at 3,253,065 to 3,253,229
yhaL
b4470: yhaM - hypothetical protein (NCBI), at 3,253,363 to 3,254,673
yhaM
b3110: yhaO - predicted transporter (RefSeq), at 3,254,701 to 3,256,032
yhaO
b4471: tdcG - L-serine dehydratase 3 (NCBI), at 3,256,307 to 3,257,671
tdcG
Group
Condition
b3106
b3107
b4470
b3110
b4471
yhaK
yhaL
yhaM
yhaO
tdcG
stress
octanol 0.08 vol%
-0.8
-5.3
-0.1
-0.0
-0.2
carbon source
carbon source D-Glucose 0.25mM
-0.5
-5.9
+0.2
+0.2
-0.2
phage
Ste41
-0.4
-4.1
-0.1
+0.2
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-4.6
+0.2
-0.2
+0.1
motility
outer cut, LB soft agar motility assay
+0.8
-3.3
-1.0
+0.0
+0.2
protonated/deuterated
deuterated glucose in H2O
-0.1
-3.5
-0.1
+0.1
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.3
-1.4
-0.4
-0.2
+0.1
phage
N4_phage 0.09375 MOI TC1
-0.2
-2.5
+0.0
-0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.6
-1.8
+0.3
-0.0
-0.2
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.1
-1.7
-0.6
+0.4
-0.3
stress
3-methyl-1-butanol 2.5 vol%
+0.1
-1.9
+0.0
+0.1
-0.1
phage
T7_phage 0.00015625 MOI TC4
-0.3
-1.1
+0.3
-0.1
-0.6
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.4
-2.0
-0.1
+0.1
+0.0
control
PBS_cage_2_mouse_2_Cecum_40 hr
-0.2
-1.4
-0.3
+0.2
+0.3
no phage control
Control_TnG
-0.3
-1.6
+0.2
+0.1
+0.1
phage
Lambda_ci1857
-1.3
-0.5
+0.2
+0.2
-0.1
carbon source
carbon source D-Glucose 5mM
+0.4
-2.2
+0.3
+0.3
-0.1
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
+0.4
-1.9
+0.2
-0.0
+0.1
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.4
-1.1
-0.9
+0.2
+0.3
gfp_control
GFP_control
-0.1
-1.4
-0.2
+0.3
+0.3
motility
outer cut, LB soft agar motility assay
+0.5
-0.7
-0.6
+0.3
+0.3
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.7
+1.3
-0.1
+0.1
-0.1
carbon source
carbon source D-Mannose 0.5mM
-0.4
+1.4
-0.1
-0.1
+0.1
nitrogen source
L-Arginine (N)
+1.7
+0.2
-0.2
-0.2
-0.0
carbon source
carbon source D-Glucose 20mM
+0.1
+1.4
-0.4
+0.0
+0.3
carbon source
a-Ketoglutaric (C)
+1.0
+0.7
+0.0
-0.2
+0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.3
+2.8
-0.2
-0.0
-0.2
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.2
+2.3
-0.6
+0.0
+0.5
nitrogen source
L-Arginine (N)
+1.8
+0.4
-0.0
-0.0
+0.1
stress
Chlorite 1.25 mM
+0.5
-0.8
+0.4
-0.3
+3.2
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