Fitness for 5 genes in Escherichia coli BW25113

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500 ntyhaK and yhaL are separated by 22 nucleotidesyhaL and yhaM are separated by 133 nucleotidesyhaM and yhaO are separated by 27 nucleotidesyhaO and tdcG are separated by 274 nucleotides b3106: yhaK - predicted pirin-related protein (NCBI), at 3,252,341 to 3,253,042 yhaK b3107: yhaL - orf, hypothetical protein (VIMSS), at 3,253,065 to 3,253,229 yhaL b4470: yhaM - hypothetical protein (NCBI), at 3,253,363 to 3,254,673 yhaM b3110: yhaO - predicted transporter (RefSeq), at 3,254,701 to 3,256,032 yhaO b4471: tdcG - L-serine dehydratase 3 (NCBI), at 3,256,307 to 3,257,671 tdcG
Group Condition b3106 b3107 b4470 b3110 b4471
yhaK yhaL yhaM yhaO tdcG
stress octanol 0.08 vol% -0.8 -5.3 -0.1 -0.0 -0.2
carbon source carbon source D-Glucose 0.25mM -0.5 -5.9 +0.2 +0.2 -0.2
phage Ste41 -0.4 -4.1 -0.1 +0.2 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -4.6 +0.2 -0.2 +0.1
motility outer cut, LB soft agar motility assay +0.8 -3.3 -1.0 +0.0 +0.2
protonated/deuterated deuterated glucose in H2O -0.1 -3.5 -0.1 +0.1 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.3 -1.4 -0.4 -0.2 +0.1
phage N4_phage 0.09375 MOI TC1 -0.2 -2.5 +0.0 -0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -1.8 +0.3 -0.0 -0.2
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.1 -1.7 -0.6 +0.4 -0.3
stress 3-methyl-1-butanol 2.5 vol% +0.1 -1.9 +0.0 +0.1 -0.1
phage T7_phage 0.00015625 MOI TC4 -0.3 -1.1 +0.3 -0.1 -0.6
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.4 -2.0 -0.1 +0.1 +0.0
control PBS_cage_2_mouse_2_Cecum_40 hr -0.2 -1.4 -0.3 +0.2 +0.3
no phage control Control_TnG -0.3 -1.6 +0.2 +0.1 +0.1
phage Lambda_ci1857 -1.3 -0.5 +0.2 +0.2 -0.1
carbon source carbon source D-Glucose 5mM +0.4 -2.2 +0.3 +0.3 -0.1
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.4 -1.9 +0.2 -0.0 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 -1.1 -0.9 +0.2 +0.3
gfp_control GFP_control -0.1 -1.4 -0.2 +0.3 +0.3
motility outer cut, LB soft agar motility assay +0.5 -0.7 -0.6 +0.3 +0.3
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.7 +1.3 -0.1 +0.1 -0.1
carbon source carbon source D-Mannose 0.5mM -0.4 +1.4 -0.1 -0.1 +0.1
nitrogen source L-Arginine (N) +1.7 +0.2 -0.2 -0.2 -0.0
carbon source carbon source D-Glucose 20mM +0.1 +1.4 -0.4 +0.0 +0.3
carbon source a-Ketoglutaric (C) +1.0 +0.7 +0.0 -0.2 +0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.3 +2.8 -0.2 -0.0 -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr -0.2 +2.3 -0.6 +0.0 +0.5
nitrogen source L-Arginine (N) +1.8 +0.4 -0.0 -0.0 +0.1
stress Chlorite 1.25 mM +0.5 -0.8 +0.4 -0.3 +3.2
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