Fitness for 5 genes in Escherichia coli BW25113

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500 ntyghG and b2972 are separated by 65 nucleotidesb2972 and yghJ are separated by 197 nucleotidesyghJ and yghK are separated by 484 nucleotidesyghK and glcB are separated by 354 nucleotides b2971: yghG - hypothetical protein (NCBI), at 3,111,089 to 3,111,499 yghG b2972: b2972 - putative peptidase (VIMSS), at 3,111,565 to 3,112,374 b2972 b4466: yghJ - predicted inner membrane lipoprotein (NCBI), at 3,112,572 to 3,117,134 yghJ b2975: yghK - glycolate transporter (NCBI), at 3,117,619 to 3,119,301 yghK b2976: glcB - malate synthase (NCBI), at 3,119,656 to 3,121,827 glcB
Group Condition b2971 b2972 b4466 b2975 b2976
yghG b2972 yghJ yghK glcB
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 -0.5 +0.1 -0.4 +0.4
phage T7_phage 0.00015625 MOI TC3 +0.1 -0.5 -0.5 -0.1 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.7 -0.5 +0.0 -0.2 +0.4
stress Benzalkonium Chloride 0.004 mg/ml -0.5 -0.3 -0.0 +0.3 -0.4
phage T7_phage 0.00015625 MOI TC4 +0.0 -0.2 -0.2 -0.4 -0.2
plasmid transformation vcDART vector transformation; agar assay -0.4 -0.2 -0.2 -0.2 +0.3
plasmid transformation vcDART vector transformation; agar assay -0.3 -0.2 -0.1 -0.2 +0.2
plasmid transformation mariner vector transformation; agar assay -0.2 -0.3 -0.1 -0.2 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.4 +0.1 -0.1 +0.3
phage Ste41 +0.2 +0.4 -0.2 -0.5 -0.3
stress Aluminum chloride 5 mM +0.4 -0.2 -0.1 -0.0 -0.4
predation 3 hr rep1_Tiberius_100fold_dilution -0.4 +0.2 +0.2 -0.2 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.6 +0.2 -0.1 +0.0 +0.2
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.4 -0.2 +0.1 -0.1 +0.4
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.6 +0.1 +0.1 +0.2 +0.1
phage Ste41 +0.2 +0.3 -0.1 -0.3 -0.1
carbon source carbon source D-Mannose 1mM +0.4 -0.5 -0.0 +0.2 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 +0.1 +0.4 -0.2 +0.4
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.6 -0.3 +0.0 +0.0 -0.1
phage RB68 +0.5 +0.2 -0.1 -0.3 -0.1
carbon source Ying_Others16 rep B; time point 2 +0.5 -0.2 +0.0 -0.1 +0.2
phage RB68 +0.6 +0.5 +0.1 -0.2 -0.1
control PBS_cage_1_mice _Feces_22 hr -0.0 +0.1 -0.1 +0.1 +0.8
carbon source carbon source D-Glucose 5mM +0.4 +0.1 +0.1 +0.0 +0.4
phage R2_N4_T2_phage10 +0.7 +0.0 +0.2 -0.1 +0.2
phage N4_phage 0.00009375 MOI TC4 +0.4 +0.2 +0.1 +0.3 +0.2
control PBS_cage_1_mice _Feces_40 hr +0.1 +0.4 -0.1 +0.1 +0.6
control PBS_cage_1_mice _Feces_16 hr +0.1 +0.3 +0.0 +0.0 +0.8
carbon source Glycolic Acid (C) +0.0 -0.1 +0.1 -0.6 +2.1
carbon source Glycolic Acid (C) +0.2 +0.1 +0.1 -0.5 +2.5
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