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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ygfO and guaD are separated by 14 nucleotides
guaD and ygfQ are separated by 35 nucleotides
ygfQ and ygfS are separated by 35 nucleotides
ygfS and ygfT overlap by 1 nucleotides
b2882: ygfO - putative transport protein (VIMSS), at 3,022,373 to 3,023,773
ygfO
b2883: guaD - guanine deaminase (NCBI), at 3,023,788 to 3,025,107
guaD
b4464: ygfQ - predicted transporter (NCBI), at 3,025,143 to 3,026,510
ygfQ
b2886: ygfS - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,026,546 to 3,027,034
ygfS
b2887: ygfT - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,027,034 to 3,028,953
ygfT
Group
Condition
b2882
b2883
b4464
b2886
b2887
ygfO
guaD
ygfQ
ygfS
ygfT
phage
Ste41
-0.1
-0.0
-0.5
-1.1
-0.0
phage
N4_phage 0.009375 MOI TC2
-0.4
-0.3
-0.3
-0.2
-0.4
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.2
-0.0
-0.4
-0.6
-0.1
phage
T7_phage 0.00015625 MOI TC4
+0.3
-0.1
-0.8
-0.6
-0.1
stress
Cisplatin 0.1 mg/ml
-0.2
-0.2
-0.8
-0.0
+0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.5
-0.4
+0.2
-0.0
-0.3
phage
P2_phage_0.5625_MOI
+0.3
-0.2
+0.1
-0.6
-0.2
control
PBS_cage_1_mice _Feces_16 hr
-0.1
-0.3
+0.3
-0.6
+0.2
phage
T7_phage 0.0015625 MOI TC1
+0.5
-0.1
-0.4
-0.3
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
-0.5
+0.0
-0.3
+0.5
control
PBS_cage_1_mice _Feces_22 hr
-0.1
-0.0
+0.4
-0.6
+0.1
phage
Ste41
-0.2
-0.5
-0.6
+0.4
+0.6
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.1
+0.5
-0.8
-0.3
+0.3
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.0
+0.0
+0.4
-0.9
+0.4
sir2-hera
SIR2-HerA
-0.1
-0.2
+0.5
-0.4
+0.2
carbon source
carbon source D-Glucose 5mM
+0.4
+0.4
-0.8
-0.1
+0.1
carbon source
carbon source D-Mannose 5mM
+0.1
+0.8
-0.1
-0.6
-0.1
phage
TP4
+0.1
+0.4
-0.5
-0.1
+0.2
nitrogen source
Glycine (N)
-0.1
+0.2
-0.5
+0.5
+0.0
phage
T7_phage 0.0015625 MOI TC2
-0.1
-0.7
+0.2
+0.7
-0.0
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.3
-0.0
-0.3
+0.7
+0.2
stress
octanol 0.08 vol%
+0.1
+0.2
-0.0
+0.8
-0.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.1
+0.2
-0.7
+0.8
+0.3
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.3
-0.1
-0.3
+1.2
+0.2
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.3
+0.0
+0.4
+0.4
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.3
+0.2
+0.1
+0.9
+0.0
carbon source
carbon source D-Mannose 0.5mM
+0.4
-0.2
+0.6
-0.1
+0.4
predation
48 hr rep3_Tiberius_100fold_dilution
-0.1
+0.2
+0.4
+0.6
+0.2
stress
3-methyl-1-butanol 1.25 vol%
-0.1
+0.0
+0.2
+0.8
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.6
+0.4
+0.2
+0.2
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