Fitness for 5 genes in Escherichia coli BW25113

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500 ntygfO and guaD are separated by 14 nucleotidesguaD and ygfQ are separated by 35 nucleotidesygfQ and ygfS are separated by 35 nucleotidesygfS and ygfT overlap by 1 nucleotides b2882: ygfO - putative transport protein (VIMSS), at 3,022,373 to 3,023,773 ygfO b2883: guaD - guanine deaminase (NCBI), at 3,023,788 to 3,025,107 guaD b4464: ygfQ - predicted transporter (NCBI), at 3,025,143 to 3,026,510 ygfQ b2886: ygfS - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,026,546 to 3,027,034 ygfS b2887: ygfT - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,027,034 to 3,028,953 ygfT
Group Condition b2882 b2883 b4464 b2886 b2887
ygfO guaD ygfQ ygfS ygfT
phage Ste41 -0.1 -0.0 -0.5 -1.1 -0.0
phage N4_phage 0.009375 MOI TC2 -0.4 -0.3 -0.3 -0.2 -0.4
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.2 -0.0 -0.4 -0.6 -0.1
phage T7_phage 0.00015625 MOI TC4 +0.3 -0.1 -0.8 -0.6 -0.1
stress Cisplatin 0.1 mg/ml -0.2 -0.2 -0.8 -0.0 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 -0.4 +0.2 -0.0 -0.3
phage P2_phage_0.5625_MOI +0.3 -0.2 +0.1 -0.6 -0.2
control PBS_cage_1_mice _Feces_16 hr -0.1 -0.3 +0.3 -0.6 +0.2
phage T7_phage 0.0015625 MOI TC1 +0.5 -0.1 -0.4 -0.3 -0.2
motility outer cut, LB soft agar motility assay -0.1 -0.5 +0.0 -0.3 +0.5
control PBS_cage_1_mice _Feces_22 hr -0.1 -0.0 +0.4 -0.6 +0.1
phage Ste41 -0.2 -0.5 -0.6 +0.4 +0.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.1 +0.5 -0.8 -0.3 +0.3
control PBS_cage_1_mouse_1_Cecum_40 hr +0.0 +0.0 +0.4 -0.9 +0.4
sir2-hera SIR2-HerA -0.1 -0.2 +0.5 -0.4 +0.2
carbon source carbon source D-Glucose 5mM +0.4 +0.4 -0.8 -0.1 +0.1
carbon source carbon source D-Mannose 5mM +0.1 +0.8 -0.1 -0.6 -0.1
phage TP4 +0.1 +0.4 -0.5 -0.1 +0.2
nitrogen source Glycine (N) -0.1 +0.2 -0.5 +0.5 +0.0
phage T7_phage 0.0015625 MOI TC2 -0.1 -0.7 +0.2 +0.7 -0.0
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.3 -0.0 -0.3 +0.7 +0.2
stress octanol 0.08 vol% +0.1 +0.2 -0.0 +0.8 -0.6
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.1 +0.2 -0.7 +0.8 +0.3
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.3 -0.1 -0.3 +1.2 +0.2
stress LB Anaerobic with Ampicillin 0.00000195313 mM -0.3 +0.0 +0.4 +0.4 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.3 +0.2 +0.1 +0.9 +0.0
carbon source carbon source D-Mannose 0.5mM +0.4 -0.2 +0.6 -0.1 +0.4
predation 48 hr rep3_Tiberius_100fold_dilution -0.1 +0.2 +0.4 +0.6 +0.2
stress 3-methyl-1-butanol 1.25 vol% -0.1 +0.0 +0.2 +0.8 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.6 +0.4 +0.2 +0.2
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