Fitness for 5 genes in Escherichia coli BW25113

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500 ntinsC and ygeQ are separated by 91 nucleotidesygeQ and glyU are separated by 155 nucleotidesglyU and b2865 are separated by 78 nucleotidesb2865 and xdhA are separated by 453 nucleotides b2861: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 2,995,257 to 2,995,622 insC b2863: ygeQ - orf, hypothetical protein (VIMSS), at 2,995,714 to 2,996,850 ygeQ b2864: glyU - tRNA-Gly (NCBI), at 2,997,006 to 2,997,079 glyU b2865: b2865 - putative lipoprotein (VIMSS), at 2,997,158 to 2,997,913 b2865 b2866: xdhA - xanthine dehydrogenase, molybdenum binding subunit (NCBI), at 2,998,367 to 3,000,625 xdhA
Group Condition b2861 b2863 b2864 b2865 b2866
insC ygeQ glyU b2865 xdhA
phage R2_N4_T1_phage10 N.D. -0.4 N.D. -0.4 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.4 N.D. -0.8 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.5 N.D. -0.8 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. -0.3 N.D. -0.7 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.5 N.D. -0.6 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.5 N.D. -0.3 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.7 N.D. -0.1 -0.1
phage N4_phage 0.009375 MOI TC2 N.D. -0.2 N.D. -0.4 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.8 N.D. -0.2 +0.1
carbon source carbon source D-Glucose 0.25mM N.D. -0.4 N.D. -0.2 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. -0.2 N.D. -0.6 +0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.4 N.D. -0.5 +0.1
carbon source carbon source D-Glucose 0.25mM N.D. -0.5 N.D. -0.3 +0.1
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM N.D. -0.4 N.D. -0.3 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -0.4 N.D. -0.2 +0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. -0.3 N.D. +0.3 -0.3
stress LB Anaerobic with Spectinomycin 0.0000625 mM N.D. -0.3 N.D. -0.2 +0.3
motility outer cut, LB soft agar motility assay N.D. -0.4 N.D. +0.2 +0.2
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. +0.4 N.D. +0.1 -0.4
phage RB68 N.D. +0.4 N.D. +0.2 -0.2
phage RB68 N.D. +0.4 N.D. +0.4 -0.2
control PBS_cage_1_mice _Feces_16 hr N.D. -0.0 N.D. +0.4 +0.3
phage Ste41 N.D. +0.3 N.D. +0.1 +0.4
phage Ste41 N.D. +0.3 N.D. +0.7 -0.2
phage T7_phage 0.0015625 MOI TC1 N.D. +0.2 N.D. +0.5 +0.2
motility inner cut, LB soft agar motility assay N.D. +0.2 N.D. +0.4 +0.4
phage T7_phage 0.00015625 MOI TC4 N.D. +0.2 N.D. +0.6 +0.3
stress Phosphomycin 0.001 mg/ml N.D. +0.5 N.D. +0.4 +0.3
stress Bacitracin 1 mg/ml N.D. +0.2 N.D. +1.1 +0.1
phage T7_phage 0.0015625 MOI TC2 N.D. +0.6 N.D. +0.5 +0.3
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