Fitness for 5 genes in Escherichia coli BW25113

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500 ntgrpE and yfjB are separated by 122 nucleotidesyfjB and recN are separated by 85 nucleotidesrecN and smpA are separated by 148 nucleotidessmpA and b2618 are separated by 61 nucleotides b2614: grpE - heat shock protein (NCBI), at 2,748,137 to 2,748,730 grpE b2615: yfjB - inorganic polyphosphate/ATP-NAD kinase (NCBI), at 2,748,853 to 2,749,731 yfjB b2616: recN - recombination and repair protein (NCBI), at 2,749,817 to 2,751,478 recN b2617: smpA - small membrane protein A (VIMSS), at 2,751,627 to 2,751,968 smpA b2618: b2618 - orf, hypothetical protein (VIMSS), at 2,752,030 to 2,752,320 b2618
Group Condition b2614 b2615 b2616 b2617 b2618
grpE yfjB recN smpA b2618
stress Cisplatin 0.1 mg/ml -0.6 N.D. -4.7 -4.4 -0.1
phage T7_phage 0.00015625 MOI TC2 -0.2 N.D. -0.2 -6.1 -1.5
stress Nalidixic 0.06 mM -0.6 N.D. -2.0 -6.8 +1.6
stress Nalidixic 0.006 mg/ml -0.4 N.D. -3.2 -5.4 +1.3
phage T7_phage 0.0015625 MOI TC1 -0.5 N.D. +0.4 -6.5 -0.4
stress LB + carbenicillin regrowth after 48hr -0.2 N.D. -5.7 -0.9 +0.2
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -1.1 N.D. -1.9 -1.3 -2.2
stress LB + carbenicillin regrowth after 48hr -0.2 N.D. -5.4 -0.9 +0.3
stress Nalidixic 0.06 mM -0.0 N.D. -2.3 -5.4 +1.7
phage T7_phage 0.000015625 MOI TC3 +0.2 N.D. -0.3 -5.5 -0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON -4.7 N.D. -0.4 +0.8 -1.3
phage T7_phage 0.00015625 MOI TC4 -0.7 N.D. +0.6 -4.2 -1.0
stress Nalidixic 0.0313 mM -0.1 N.D. -3.3 -2.9 +1.1
phage phage -1.2 N.D. -0.1 -4.4 +0.7
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 N.D. -0.1 -5.3 +0.6
stress LB Anaerobic with Nalidixic 0.0000078125 mM -0.1 N.D. -1.6 -3.5 +0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.2 N.D. -0.5 -2.2 -3.0
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.8 N.D. -0.1 -4.2 -0.9
stress LB Aerobic with Spiramycin 0.000125 mM +0.4 N.D. -0.0 -5.6 +1.0
stress LB Aerobic with Ramoplanin 0.000125 mM +0.2 N.D. -0.2 -5.6 +1.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.9 N.D. -0.0 -3.3 +1.2
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM +1.1 N.D. +0.0 -5.2 +0.0
stress LB Aerobic with Novobiocin 0.0000625 mM -1.6 N.D. +0.1 -3.2 +1.2
stress Bacitracin 0.5 mg/ml +1.0 N.D. -0.1 -4.7 +0.8
stress LB Aerobic with Ramoplanin 0.000125 mM +1.0 N.D. -0.2 -5.6 +1.9
stress octanol 0.08 vol% +0.7 N.D. -0.2 -6.4 +3.4
stress Chloramphenicol 0.001 mg/ml -0.4 N.D. -0.1 -3.6 +2.2
stress Benzalkonium Chloride 0.004 mg/ml -0.5 N.D. -0.0 -4.1 +4.5
stress Cephalothin 0.01 mg/ml -0.9 N.D. +0.1 +4.9 +1.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.8 N.D. +0.2 +5.2 +1.0
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