Fitness for 5 genes in Escherichia coli BW25113

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500 ntffh and ypjD are separated by 166 nucleotidesypjD and yfjD are separated by 20 nucleotidesyfjD and grpE are separated by 54 nucleotidesgrpE and yfjB are separated by 122 nucleotides b2610: ffh - Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) (NCBI), at 2,744,456 to 2,745,817 ffh b2611: ypjD - predicted inner membrane protein (RefSeq), at 2,745,984 to 2,746,775 ypjD b4461: yfjD - predicted inner membrane protein (RefSeq), at 2,746,796 to 2,748,082 yfjD b2614: grpE - heat shock protein (NCBI), at 2,748,137 to 2,748,730 grpE b2615: yfjB - inorganic polyphosphate/ATP-NAD kinase (NCBI), at 2,748,853 to 2,749,731 yfjB
Group Condition b2610 b2611 b4461 b2614 b2615
ffh ypjD yfjD grpE yfjB
predation 6 hr rep2_HD100_10fold_dilution_grewON N.D. -0.5 +0.1 -4.8 N.D.
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. +0.4 +0.4 -4.7 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. -0.8 -0.3 -1.9 N.D.
phage RB68 N.D. -0.9 -0.2 -1.6 N.D.
m9 anaerobic no stress control M9 anaerobic no stress control N.D. -0.7 -0.1 -1.5 N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM N.D. +0.0 -0.6 -1.6 N.D.
phage N4_phage 0.09375 MOI TC1 N.D. +0.2 -0.1 -2.3 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.1 +0.3 -2.4 N.D.
M9 Acetate 0.5% aerobic N.D. -1.7 +0.3 -0.5 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.4 +0.2 -1.7 N.D.
sulfur source M63 no sulfur + 5 mM sulfate N.D. -0.6 +0.2 -1.4 N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. +0.2 +0.0 -1.9 N.D.
stress M9 with Methotrexate 0.06 mM 48 hr N.D. -1.7 -0.1 +0.3 N.D.
carbon source Glycolic Acid (C) N.D. -0.8 +0.9 -1.3 N.D.
carbon source acetate (C) N.D. -1.7 +0.5 +0.1 N.D.
motility outer cut, LB soft agar motility assay N.D. +0.9 +0.2 -2.0 N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. +0.4 +0.3 -1.7 N.D.
carbon source Glycolic Acid (C) N.D. -0.5 +0.9 -0.9 N.D.
nitrogen source Ammonium chloride (N) N.D. -1.2 -0.0 +0.9 N.D.
carbon source carbon source D-Glucose 5mM N.D. +0.8 +0.6 -1.7 N.D.
carbon source L-Malic (C) N.D. -1.2 +0.3 +0.8 N.D.
carbon source D-Serine (C) N.D. -1.1 +0.4 +0.6 N.D.
stress M9 with Methotrexate 1.98 mM 48 hr N.D. -1.1 +0.3 +0.7 N.D.
stress M9 with Methotrexate 1.98 mM 24 hr N.D. -0.9 +0.5 +0.8 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. -0.9 +0.9 +0.6 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. -1.0 +0.5 +1.4 N.D.
stress Nickel (II) chloride 1.5 mM N.D. +0.1 +2.0 -0.1 N.D.
stress octanol 0.08 vol% N.D. +0.6 +0.9 +0.7 N.D.
stress Cobalt chloride 0.32 mM N.D. +0.2 +1.0 +1.0 N.D.
stress Nickel (II) chloride 1 mM N.D. +0.6 +2.7 +0.6 N.D.
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