Fitness for 5 genes in Escherichia coli BW25113

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500 ntyfhM and sseA are separated by 206 nucleotidessseA and ryfA are separated by 515 nucleotidesryfA and sseB overlap by 2 nucleotidessseB and pepB are separated by 141 nucleotides b2520: yfhM - hypothetical protein (NCBI), at 2,645,348 to 2,650,309 yfhM b2521: sseA - putative thiosulfate sulfurtransferase (VIMSS), at 2,650,516 to 2,651,361 sseA b4440: ryfA - unknown RNA (NCBI), at 2,651,877 to 2,652,180 ryfA b2522: sseB - enhanced serine sensitivity (VIMSS), at 2,652,179 to 2,652,955 sseB b2523: pepB - putative peptidase (VIMSS), at 2,653,097 to 2,654,380 pepB
Group Condition b2520 b2521 b4440 b2522 b2523
yfhM sseA ryfA sseB pepB
phage Ste41 -0.0 -0.1 -0.1 -0.4 -1.3
stress 2,4-Dinitrophenol 2 mM -0.1 -0.5 -0.0 -0.5 -0.2
phage RB68 -0.1 -0.2 -0.4 -0.3 -0.2
stress Cephalothin 0.01 mg/ml -0.4 -0.4 -0.1 +0.1 -0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.3 -0.7 -0.1 +0.2 +0.3
phage T7_phage 0.0015625 MOI TC1 +0.2 -0.2 +0.0 +0.2 -0.5
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.1 +0.4 +0.0 +0.1 -0.6
stress Cisplatin 0.05 mg/ml -0.0 -0.1 +0.1 +0.4 -0.5
stress Cisplatin 0.1 mg/ml +0.1 +0.4 -0.3 +0.2 -0.5
stress 3-methyl-1-butanol 1.25 vol% -0.3 +0.3 +0.3 -0.1 -0.1
phage T7_phage 0.0015625 MOI TC2 +0.0 -0.0 +0.5 +0.2 -0.4
stress Nickel (II) chloride 1.5 mM -0.0 +0.9 +0.0 -0.0 -0.5
phage T7_phage 0.00015625 MOI TC3 -0.0 -0.6 +0.5 +0.3 +0.3
stress Nickel (II) chloride 1.5 mM -0.0 +1.0 +0.1 -0.1 -0.2
stress Nickel (II) chloride 1.5 mM -0.0 +1.1 +0.1 +0.0 -0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 +0.2 -0.2 +0.3 +0.5
plasmid transformation mariner vector transformation; agar assay +0.2 +0.1 +0.1 +0.5 +0.2
plasmid transformation mariner vector transformation; agar assay +0.0 +0.3 +0.0 +0.5 +0.3
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.1 +0.7 +0.1 +0.2 +0.2
carbon source Ying_Nucleobase14 rep B; time point 3 +0.0 +0.6 +0.1 +0.2 +0.5
plasmid transformation vcDART vector transformation; agar assay -0.0 +0.1 -0.0 +1.0 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.0 -0.3 +1.2 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.3 -0.2 +0.9 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -0.2 +0.2 +1.2 +0.6
plasmid transformation vcDART vector transformation; agar assay +0.0 +0.2 +0.0 +1.4 +0.7
plasmid transformation vcDART vector transformation; agar assay +0.0 +0.2 +0.0 +1.4 +0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.3 +0.2 +1.3 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.0 -0.2 +2.1 +0.9
stress Nickel (II) chloride 1 mM -0.0 +3.1 -0.0 +0.2 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.2 +0.1 +1.9 +0.8
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