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Cofit
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yfhM and sseA are separated by 206 nucleotides
sseA and ryfA are separated by 515 nucleotides
ryfA and sseB overlap by 2 nucleotides
sseB and pepB are separated by 141 nucleotides
b2520: yfhM - hypothetical protein (NCBI), at 2,645,348 to 2,650,309
yfhM
b2521: sseA - putative thiosulfate sulfurtransferase (VIMSS), at 2,650,516 to 2,651,361
sseA
b4440: ryfA - unknown RNA (NCBI), at 2,651,877 to 2,652,180
ryfA
b2522: sseB - enhanced serine sensitivity (VIMSS), at 2,652,179 to 2,652,955
sseB
b2523: pepB - putative peptidase (VIMSS), at 2,653,097 to 2,654,380
pepB
Group
Condition
b2520
b2521
b4440
b2522
b2523
yfhM
sseA
ryfA
sseB
pepB
phage
Ste41
-0.0
-0.1
-0.1
-0.4
-1.3
stress
2,4-Dinitrophenol 2 mM
-0.1
-0.5
-0.0
-0.5
-0.2
phage
RB68
-0.1
-0.2
-0.4
-0.3
-0.2
stress
Cephalothin 0.01 mg/ml
-0.4
-0.4
-0.1
+0.1
-0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.3
-0.7
-0.1
+0.2
+0.3
phage
T7_phage 0.0015625 MOI TC1
+0.2
-0.2
+0.0
+0.2
-0.5
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.1
+0.4
+0.0
+0.1
-0.6
stress
Cisplatin 0.05 mg/ml
-0.0
-0.1
+0.1
+0.4
-0.5
stress
Cisplatin 0.1 mg/ml
+0.1
+0.4
-0.3
+0.2
-0.5
stress
3-methyl-1-butanol 1.25 vol%
-0.3
+0.3
+0.3
-0.1
-0.1
phage
T7_phage 0.0015625 MOI TC2
+0.0
-0.0
+0.5
+0.2
-0.4
stress
Nickel (II) chloride 1.5 mM
-0.0
+0.9
+0.0
-0.0
-0.5
phage
T7_phage 0.00015625 MOI TC3
-0.0
-0.6
+0.5
+0.3
+0.3
stress
Nickel (II) chloride 1.5 mM
-0.0
+1.0
+0.1
-0.1
-0.2
stress
Nickel (II) chloride 1.5 mM
-0.0
+1.1
+0.1
+0.0
-0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
+0.2
-0.2
+0.3
+0.5
plasmid transformation
mariner vector transformation; agar assay
+0.2
+0.1
+0.1
+0.5
+0.2
plasmid transformation
mariner vector transformation; agar assay
+0.0
+0.3
+0.0
+0.5
+0.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.1
+0.7
+0.1
+0.2
+0.2
carbon source
Ying_Nucleobase14 rep B; time point 3
+0.0
+0.6
+0.1
+0.2
+0.5
plasmid transformation
vcDART vector transformation; agar assay
-0.0
+0.1
-0.0
+1.0
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.0
-0.3
+1.2
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.3
-0.2
+0.9
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-0.2
+0.2
+1.2
+0.6
plasmid transformation
vcDART vector transformation; agar assay
+0.0
+0.2
+0.0
+1.4
+0.7
plasmid transformation
vcDART vector transformation; agar assay
+0.0
+0.2
+0.0
+1.4
+0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
+0.3
+0.2
+1.3
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.0
-0.2
+2.1
+0.9
stress
Nickel (II) chloride 1 mM
-0.0
+3.1
-0.0
+0.2
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.2
+0.1
+1.9
+0.8
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