Fitness for 5 genes in Escherichia coli BW25113

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500 ntpurR and ydhB overlap by 4 nucleotidesydhB and ydhC are separated by 112 nucleotidesydhC and cfa are separated by 290 nucleotidescfa and ribC are separated by 39 nucleotides b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893 purR b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822 ydhB b1660: ydhC - predicted transporter (NCBI), at 1,737,935 to 1,739,146 ydhC b1661: cfa - cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI), at 1,739,437 to 1,740,585 cfa b1662: ribC - riboflavin synthase subunit alpha (NCBI), at 1,740,625 to 1,741,266 ribC
Group Condition b1658 b1659 b1660 b1661 b1662
purR ydhB ydhC cfa ribC
stress Benzalkonium Chloride 0.004 mg/ml -3.9 -2.1 -0.3 +0.2 N.D.
carbon source Ying_Nucleobase14 rep C; time point 3 -1.3 -1.6 -1.7 -0.3 N.D.
motility outer cut, LB soft agar motility assay -2.8 -1.4 -1.0 +0.7 N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 -1.2 -2.1 -1.3 +0.1 N.D.
carbon source Ying_Nucleobase14 rep A; time point 3 -1.3 -1.1 -1.7 -0.3 N.D.
carbon source Ying_Nucleobase14 rep B; time point 2 -0.9 -1.8 -1.6 -0.1 N.D.
stress Trimethoprim 0.0025 mM -3.3 -0.4 +0.3 -0.8 N.D.
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.3 -1.9 -2.3 +0.1 N.D.
carbon source Ying_Nucleobase14 rep A; time point 2 -0.8 -1.1 -1.7 -0.2 N.D.
carbon source Ying_Nucleobase14 rep C; time point 2 -1.1 -1.4 -1.1 -0.2 N.D.
carbon source Adenosine 0.3742 mM (C) -0.6 -1.7 -1.2 -0.3 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM -3.9 -0.1 +0.1 +0.3 N.D.
carbon source Adenosine 0.3742 mM (C) -0.4 -1.3 -1.5 -0.3 N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -2.4 -0.3 -0.5 +0.1 N.D.
carbon source Inosine 0.3728 mM (C) -0.6 -1.7 -1.0 +0.2 N.D.
stress octanol 0.08 vol% -3.5 +0.9 +0.3 -0.5 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -3.4 -0.0 +1.0 -0.2 N.D.
stress Trimethoprim 0.00125 mM -2.7 +0.3 +0.1 -0.1 N.D.
carbon source Inosine 0.3728 mM (C) -0.3 -1.8 -0.7 +0.5 N.D.
stress 2,4-Dinitrophenol 2 mM -2.6 -0.4 -0.1 +0.9 N.D.
stress 2,4-Dinitrophenol 2 mM -2.7 +0.0 -0.1 +1.3 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -2.1 -0.8 +0.3 +1.1 N.D.
stress acetate 140 mM -1.7 +0.3 +0.7 +0.5 N.D.
nitrogen source Glycine (N) +1.7 +0.0 +0.5 -1.5 N.D.
phage Ste41 -1.3 +2.2 +0.4 -0.1 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +2.0 -1.0 +0.9 -0.3 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.3 +0.1 -0.8 +1.0 N.D.
plasmid transformation vcDART vector transformation; agar assay +2.8 -0.3 +0.3 +0.2 N.D.
phage Laval1267 +0.3 +0.5 +0.3 +2.1 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +2.8 +0.2 +0.4 +0.5 N.D.
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