Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjjU and yjjV overlap by 4 nucleotidesyjjV and yjjW are separated by 419 nucleotidesyjjW and yjjI overlap by 29 nucleotidesyjjI and deoC are separated by 257 nucleotides b4377: yjjU - predicted esterase (NCBI), at 4,610,434 to 4,611,507 yjjU b4378: yjjV - predicted DNase (NCBI), at 4,611,504 to 4,612,283 yjjV b4379: yjjW - predicted pyruvate formate lyase activating enzyme (NCBI), at 4,612,703 to 4,613,566 yjjW b4380: yjjI - hypothetical protein (NCBI), at 4,613,538 to 4,615,088 yjjI b4381: deoC - deoxyribose-phosphate aldolase (NCBI), at 4,615,346 to 4,616,125 deoC
Group Condition b4377 b4378 b4379 b4380 b4381
yjjU yjjV yjjW yjjI deoC
stress 2,4-Dinitrophenol 2 mM -0.8 -0.2 -0.1 +0.1 -1.3
stress Spectinomycin 0.0125 mg/ml -0.1 -0.2 +0.3 +0.8 -2.9
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.1 -0.5 -0.3 +0.1 -1.2
stress Spectinomycin 0.00625 mg/ml +0.3 -0.0 +0.1 +0.5 -2.6
control PBS_cage_2_mouse_1_Cecum_40 hr +0.4 -0.6 -0.6 -0.1 -0.7
motility outer cut, LB soft agar motility assay +0.4 -0.8 +0.0 +0.0 -1.2
carbon source carbon source D-Glucose 0.25mM +0.4 +0.2 -0.2 -0.7 -1.2
motility outer cut, LB soft agar motility assay -0.5 +0.5 +0.1 +0.5 -1.5
carbon source carbon source D-Mannose 0.5mM +0.2 +0.0 -0.2 +0.4 -1.3
carbon source carbon source D-Mannose 5mM -0.2 +0.6 -0.3 +0.3 -0.8
stress Cisplatin 0.1 mg/ml +1.0 -0.1 +0.2 -0.6 -0.7
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.7 -1.1 +0.5 -0.1 -0.1
stress Trimethoprim 0.005 mM -0.0 -0.6 -0.2 -0.5 +1.5
stress Benzalkonium Chloride 0.004 mg/ml +0.8 -0.2 +0.2 +0.5 -0.6
stress Aluminum chloride 5 mM +0.6 -1.1 +0.0 +0.5 +1.3
carbon source Adenosine 0.3742 mM (C) -0.2 +0.2 -0.1 +0.9 +1.0
carbon source Inosine 0.3728 mM (C) -0.2 -0.2 -0.3 +1.4 +1.1
carbon source Ying_Nucleobase14 rep C; time point 2 -0.2 -0.0 +0.1 +1.0 +1.2
phage Papa dilution 10-1 cacl2 maltose +0.4 +0.8 +0.2 +0.3 +0.4
carbon source Ying_Nucleobase14 rep B; time point 2 -0.1 +0.1 +0.0 +1.0 +1.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 +0.6 +0.2 -0.2 +1.3
carbon source Inosine 0.3728 mM (C) -0.1 +0.2 -0.1 +1.4 +1.0
carbon source Inosine 0.3728 mM (C) -0.0 +0.0 +0.1 +1.5 +0.9
carbon source Ying_Nucleobase14 rep A; time point 2 -0.1 +0.1 +0.1 +1.0 +1.4
carbon source Ying_Nucleobase14 rep A; time point 3 -0.0 -0.2 -0.1 +1.1 +1.9
carbon source Ying_Nucleobase14 rep B; time point 3 +0.0 +0.2 -0.1 +1.3 +1.6
carbon source Guanosine 0.35306 mM (C) +0.1 -0.1 -0.0 +0.8 +2.3
carbon source Ying_Nucleobase14 rep C; time point 3 -0.1 +0.2 +0.1 +1.2 +1.8
carbon source Guanosine 0.35306 mM (C) +0.0 +0.2 -0.1 +0.8 +2.3
carbon source Guanosine 0.35306 mM (C) -0.1 -0.1 +0.1 +1.0 +3.3
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