Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjiR and yjiS are separated by 176 nucleotidesyjiS and yjiT are separated by 498 nucleotidesyjiT and yjiV are separated by 2 nucleotidesyjiV and mcrC are separated by 56 nucleotides b4340: yjiR - fused predicted DNA-binding transcriptional regulator/predicted aminotransferase (NCBI), at 4,568,185 to 4,569,597 yjiR b4341: yjiS - hypothetical protein (NCBI), at 4,569,774 to 4,569,938 yjiS b4342: yjiT - orf, hypothetical protein (VIMSS), at 4,570,437 to 4,571,939 yjiT b4486: yjiV - conserved protein (pseudogene) (RefSeq), at 4,571,942 to 4,574,878 yjiV b4345: mcrC - 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI), at 4,574,935 to 4,575,981 mcrC
Group Condition b4340 b4341 b4342 b4486 b4345
yjiR yjiS yjiT yjiV mcrC
M9 Glucose 2%, 20 mM NO3- anaerobic +0.0 -0.6 -0.4 +0.0 -1.5
M9 Acetate 0.5% aerobic +0.0 -0.8 -0.5 +0.1 -1.3
M9 Glucose 2% aerobic -0.0 -0.6 -0.4 +0.0 -1.3
M9 Glucose 2% aerobic -0.0 -0.8 -0.4 +0.0 -1.1
carbon source carbon source D-Glucose 1mM -0.2 -1.0 +0.0 -0.2 -1.0
motility outer cut, LB soft agar motility assay +0.1 -1.1 -0.3 +0.1 -1.0
M9 Acetate 0.5% aerobic +0.1 -1.2 -0.5 +0.2 -0.7
M9 Glucose 2% anaerobic +0.0 -0.7 -0.4 +0.0 -1.0
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.1 -0.7 +0.1 +0.1 -1.3
motility inner cut, LB soft agar motility assay +0.3 -0.2 -0.2 +0.0 -1.7
carbon source carbon source D-Glucose 0.25mM -0.5 -2.0 -0.0 -0.0 +0.9
motility inner cut, LB soft agar motility assay +0.3 -0.3 -0.2 +0.1 -1.3
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.1 +0.3 +0.3 +0.0 -1.7
control PBS_cage_2_mice _Feces_22 hr -0.2 +0.4 +0.2 +0.1 -1.4
phage Ste41 -0.3 -1.7 +0.1 +0.1 +1.6
carbon source carbon source D-Glucose 0.25mM -0.3 -0.4 +0.1 -0.2 +1.1
control PBS_cage_1_mouse_1_Cecum_40 hr +0.2 +0.3 +0.4 +0.4 -0.9
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.0 -0.5 -0.0 -0.2 +1.3
phage T7_phage 0.0015625 MOI TC2 -0.6 +0.2 +0.4 -0.5 +1.6
msc 3hr_mediaonly_rep2_lowinoculum -0.0 -0.5 +0.0 +0.1 +1.5
phage Δdbn_10 +0.0 -0.4 -0.0 -0.1 +1.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.1 -0.4 +0.3 +0.1 +1.3
stress Trimethoprim 0.005 mM -0.1 -0.3 +0.2 -0.1 +1.5
stress Ciprofloxacin Hydrochloride 0.000156 mM +0.2 -0.5 +0.1 -0.1 +1.6
phage Papa dilution 10-1 cacl2 maltose +0.1 -0.2 -0.0 -0.3 +2.3
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr +0.1 +0.0 +0.1 +0.1 +1.7
control PBS_cage_2_mouse_2_Cecum_40 hr -0.0 +0.7 +0.2 +0.2 +1.0
phage N4_phage 0.09375 MOI TC1 -0.0 +0.0 +0.1 -0.1 +2.2
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.3 -0.2 +0.3 +0.4 +2.1
phage N4_phage 0.009375 MOI TC2 +0.1 +0.3 +0.1 -0.0 +2.4
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