Fitness for 5 genes in Escherichia coli BW25113

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500 ntfimB and fimE are separated by 477 nucleotidesfimE and fimA are separated by 481 nucleotidesfimA and fimI are separated by 64 nucleotidesfimI and fimC are separated by 36 nucleotides b4312: fimB - tyrosine recombinase/inversion of on/off regulator of fimA (NCBI), at 4,538,980 to 4,539,582 fimB b4313: fimE - tyrosine recombinase/inversion of on/off regulator of fimA (NCBI), at 4,540,060 to 4,540,656 fimE b4314: fimA - major type 1 subunit fimbrin (pilin) (NCBI), at 4,541,138 to 4,541,686 fimA b4315: fimI - fimbrial protein (VIMSS), at 4,541,751 to 4,542,290 fimI b4316: fimC - periplasmic chaperone, required for type 1 fimbriae (VIMSS), at 4,542,327 to 4,543,052 fimC
Group Condition b4312 b4313 b4314 b4315 b4316
fimB fimE fimA fimI fimC
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -1.2 +0.5 -0.8 -2.8
phage Ste41 -0.8 +3.3 -3.4 -0.4 -2.0
phage Ste41 -0.9 +3.4 -3.1 -0.2 -1.9
msc 22hr_mediaonly_rep1_highinoculum -1.4 +2.6 -1.4 +0.3 -1.4
lb LB -1.0 +3.2 -1.3 -0.8 -1.1
lb LB -1.0 +3.2 -1.3 -0.7 -1.1
msc 22hr_mediaonly_rep2_highinoculum -1.1 +2.8 -1.5 +0.4 -1.3
msc 22hr_mediaonly_rep2_lowinoculum -1.1 +2.9 -1.4 +0.3 -1.1
msc 22hr_MSC_rep2_highinoculum -1.1 +2.2 -1.0 +0.4 -0.8
lb LB -0.8 +2.7 -0.6 -0.6 -0.7
msc 22hr_mediaonly_rep1_lowinoculum -1.0 +2.9 -1.0 +0.3 -1.1
fr_1000_f3 Fr_1000_F3 -0.8 +3.2 -0.8 -0.1 -0.9
predation 3 hr rep1_Tiberius_100fold_dilution -0.7 +4.4 -1.0 -0.4 -0.9
phage LB -0.4 +3.5 -0.5 -0.4 -0.5
predation 3 hr rep1_HD100_100fold_dilution -0.7 +3.9 -0.6 -0.3 -0.7
predation 3 hr rep1_Tiberius_100fold_dilution -0.7 +4.2 -0.7 -0.2 -0.8
predation 3 hr rep1_HD100_10fold_dilution -0.7 +3.8 -0.5 -0.3 -0.5
nopredatorcontrol 3 hr NoPredatorControl -0.8 +4.4 -0.8 -0.2 -0.9
predation 3 hr rep1_Tiberius_10fold_dilution -0.7 +4.0 -0.6 -0.3 -0.6
nopredatorcontrol 3 hr NoPredatorControl -0.6 +3.9 -0.4 -0.4 -0.7
predation 3 hr rep1_HD100_10fold_dilution -0.7 +3.8 -0.5 -0.1 -0.6
predation 3 hr rep1_Tiberius_10fold_dilution -0.7 +4.4 -0.6 -0.2 -0.7
predation 3 hr rep1_HD100_no_dilution -0.6 +4.0 -0.5 -0.1 -0.5
predation 3 hr rep1_Tiberius_no_dilution -0.5 +4.2 -0.6 -0.1 -0.5
predation 3 hr rep1_HD100_100fold_dilution -0.6 +4.1 -0.6 -0.1 -0.3
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan -0.3 +4.0 -0.3 -0.3 -0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.3 +4.1 -0.4 -0.4 -0.1
mixed community Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan -0.4 +4.7 -0.3 -0.1 -0.3
mixed community Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan -0.3 +5.1 -0.3 -0.1 -0.2
nopredatorcontrol 48 hr NoPredatorControl -0.4 +5.3 -0.4 +0.1 -0.5
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