Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjhI and sgcR are separated by 302 nucleotidessgcR and sgcE are separated by 16 nucleotidessgcE and sgcA are separated by 11 nucleotidessgcA and ryjB overlap by 4 nucleotides b4299: yjhI - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,523,038 to 4,523,826 yjhI b4300: sgcR - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,524,129 to 4,524,911 sgcR b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560 sgcE b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003 sgcA b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089 ryjB
Group Condition b4299 b4300 b4301 b4302 b4624
yjhI sgcR sgcE sgcA ryjB
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -0.2 -0.7 -0.1 N.D.
carbon source carbon source D-Glucose 0.25mM -1.6 +0.1 -0.0 -0.1 N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.8 -0.6 -0.1 +0.0 N.D.
phage T7_phage 0.00015625 MOI TC4 -0.1 -0.5 -0.7 -0.3 N.D.
stress Cisplatin 0.1 mg/ml -0.1 -0.0 -0.5 -0.7 N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.7 -0.3 +0.1 -0.3 N.D.
carbon source carbon source D-Glucose 5mM -0.3 +0.3 -0.0 -1.0 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.4 -0.7 +0.2 +0.0 N.D.
phage T7_phage 0.00015625 MOI TC3 -0.3 +0.2 -0.5 -0.3 N.D.
lb LB, Aerobic -0.6 -0.4 +0.3 -0.2 N.D.
nophagecontrol R1_N4_T1_phage0 +0.3 -0.1 -0.4 -0.5 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.1 -0.3 -0.6 +0.3 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 -0.2 +0.3 -0.5 N.D.
carbon source Guanosine 0.35306 mM (C) -0.6 -0.3 +0.1 +0.3 N.D.
phage Papa dilution 10-1 cacl2 maltose +0.9 -0.3 -0.5 -0.5 N.D.
phage N4_phage 0.009375 MOI TC2 +0.5 -0.5 -0.4 -0.1 N.D.
stress LB Anaerobic with Spiramycin 0.00003125 mM -0.8 +0.1 +0.3 -0.2 N.D.
stress LB Aerobic with Tetracycline 0.00000125 mM +0.5 -0.0 -0.5 -0.4 N.D.
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.6 -0.3 +0.5 +0.0 N.D.
motility outer cut, LB soft agar motility assay -0.6 -0.4 +0.2 +0.5 N.D.
stress LB Anaerobic with Spiramycin 0.000125 mM -0.6 -0.3 +0.7 +0.0 N.D.
carbon source Inosine 0.3728 mM (C) -0.6 +0.2 +0.3 +0.1 N.D.
motility outer cut, LB soft agar motility assay -0.2 -0.3 +0.3 +0.6 N.D.
motility inner cut, LB soft agar motility assay -0.2 +0.3 +0.8 -0.3 N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM +0.7 -0.5 +0.3 +0.2 N.D.
msc 3hr_MSC_rep2_lowinoculum +0.4 +0.5 +0.1 -0.2 N.D.
control PBS_cage_2_mouse_2_Cecum_40 hr +0.5 +0.3 +0.5 -0.3 N.D.
phage RB68 -0.2 +0.5 +0.3 +0.4 N.D.
stress toluene 2.5 vol% +0.2 +0.3 -0.1 +0.6 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.0 +0.7 +0.4 +0.2 N.D.
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