Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjhG and yjhH are separated by 10 nucleotidesyjhH and yjhI are separated by 4 nucleotidesyjhI and sgcR are separated by 302 nucleotidessgcR and sgcE are separated by 16 nucleotides b4297: yjhG - KpLE2 phage-like element; predicted dehydratase (NCBI), at 4,520,150 to 4,522,117 yjhG b4298: yjhH - putative lyase/synthase (VIMSS), at 4,522,128 to 4,523,033 yjhH b4299: yjhI - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,523,038 to 4,523,826 yjhI b4300: sgcR - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,524,129 to 4,524,911 sgcR b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560 sgcE
Group Condition b4297 b4298 b4299 b4300 b4301
yjhG yjhH yjhI sgcR sgcE
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.1 -0.1 -0.8 -0.6 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.0 -0.6 -0.2 -0.7
phage T7_phage 0.00015625 MOI TC4 -0.2 +0.1 -0.1 -0.5 -0.7
carbon source carbon source D-Glucose 0.25mM +0.3 +0.1 -1.6 +0.1 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 -0.2 -0.4 -0.7 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.2 +0.1 -0.4 -0.6 +0.1
phage N4_phage 0.009375 MOI TC1 -0.1 -0.4 -0.1 +0.2 -0.4
lb LB, Aerobic +0.0 -0.2 -0.6 -0.4 +0.3
carbon source D-Serine (C) -0.7 -0.1 -0.2 +0.3 -0.1
phage R2_N4_T2_phage10 -0.1 -0.3 -0.6 +0.2 +0.1
stress LB Aerobic with Spiramycin 0.000125 mM +0.0 +0.3 -0.7 -0.3 +0.0
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.3 -0.8 -0.4 +0.1 +0.1
phage N4_phage 0.009375 MOI TC2 -0.1 -0.1 +0.5 -0.5 -0.4
phage Papa dilution 10-1 cacl2 maltose -0.3 -0.2 +0.9 -0.3 -0.5
motility outer cut, LB soft agar motility assay +0.1 +0.2 -0.6 -0.4 +0.2
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.3 +0.4 -0.1 -0.3 -0.6
phage Ste41 +0.5 -0.9 -0.3 +0.0 +0.4
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.1 -0.0 -0.6 -0.3 +0.5
stress LB Anaerobic with Spiramycin 0.000125 mM +0.0 -0.1 -0.6 -0.3 +0.7
stress LB Anaerobic with Spiramycin 0.00003125 mM -0.0 +0.1 -0.8 +0.1 +0.3
phage T7_phage 0.00015625 MOI TC3 +0.3 +0.1 -0.3 +0.2 -0.5
phage Ste41 -0.1 -0.4 +0.3 +0.3 -0.2
carbon source Inosine 0.3728 mM (C) +0.1 +0.1 -0.6 +0.2 +0.3
stress LB Anaerobic with Sulfamonomethoxine 0.00003125 mM +0.0 +0.1 -0.6 +0.1 +0.4
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 -0.2 +0.7 -0.5 +0.3
phage RB68 -0.0 -0.3 -0.2 +0.5 +0.3
nopredatorcontrol 18 hr NoPredatorControl +0.2 +0.1 -0.4 +0.2 +0.4
motility inner cut, LB soft agar motility assay +0.3 +0.1 -0.2 +0.3 +0.8
stress Spectinomycin 0.0125 mg/ml +0.1 +0.4 -0.0 +0.8 +0.1
control PBS_cage_2_mouse_2_Cecum_40 hr -0.1 +0.3 +0.5 +0.3 +0.5
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