Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjhF and yjhG are separated by 106 nucleotidesyjhG and yjhH are separated by 10 nucleotidesyjhH and yjhI are separated by 4 nucleotidesyjhI and sgcR are separated by 302 nucleotides b4296: yjhF - KpLE2 phage-like element; predicted transporter (NCBI), at 4,518,694 to 4,520,043 yjhF b4297: yjhG - KpLE2 phage-like element; predicted dehydratase (NCBI), at 4,520,150 to 4,522,117 yjhG b4298: yjhH - putative lyase/synthase (VIMSS), at 4,522,128 to 4,523,033 yjhH b4299: yjhI - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,523,038 to 4,523,826 yjhI b4300: sgcR - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,524,129 to 4,524,911 sgcR
Group Condition b4296 b4297 b4298 b4299 b4300
yjhF yjhG yjhH yjhI sgcR
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.4 -0.0 -0.2 -0.4 -0.7
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.1 -0.1 -0.1 -0.8 -0.6
lb LB, Aerobic -0.1 +0.0 -0.2 -0.6 -0.4
stress LB Aerobic with Ramoplanin 0.000125 mM -0.4 -0.0 -0.2 -0.5 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 +0.1 +0.1 -0.6 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.1 -0.2 +0.1 -0.4 -0.6
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 +0.3 -0.8 -0.4 +0.1
predation 3 hr rep1_Tiberius_10fold_dilution -0.3 -0.0 -0.2 -0.8 +0.2
phage Ste41 -0.4 +0.5 -0.9 -0.3 +0.0
predation 3 hr rep1_HD100_100fold_dilution -0.3 -0.1 +0.2 -0.7 -0.2
nopredatorcontrol 3 hr NoPredatorControl -0.2 -0.0 +0.2 -0.9 -0.1
carbon source carbon source D-Glucose 0.25mM +0.2 +0.3 +0.1 -1.6 +0.1
phage R2_N4_T2_phage10 +0.1 -0.1 -0.3 -0.6 +0.2
stress LB Aerobic with Spiramycin 0.000125 mM -0.0 +0.0 +0.3 -0.7 -0.3
carbon source D-Serine (C) -0.0 -0.7 -0.1 -0.2 +0.3
motility outer cut, LB soft agar motility assay -0.0 +0.1 +0.2 -0.6 -0.4
phage R1_N4_T2_phage8 +0.3 +0.1 -0.1 -0.4 -0.4
phage N4_phage 0.009375 MOI TC2 -0.0 -0.1 -0.1 +0.5 -0.5
carbon source D-Serine (C) +0.1 -0.6 -0.0 -0.2 +0.5
stress LB Aerobic with Irgasan 0.0000003125 mM +0.0 -0.2 -0.2 +0.7 -0.5
carbon source carbon source D-Mannose 1mM +0.3 -0.3 -0.3 +0.3 +0.1
phage Papa dilution 10-1 cacl2 maltose +0.2 -0.3 -0.2 +0.9 -0.3
msc 3hr_MSC_rep2_lowinoculum +0.1 +0.2 -0.0 +0.4 +0.5
control PBS_cage_2_mouse_2_Cecum_40 hr +0.3 -0.1 +0.3 +0.5 +0.3
phage Koxy_10 +0.3 +0.3 +0.3 +0.1 +0.2
msc 22hr_MSC_rep1_highinoculum +0.3 +0.1 +0.2 +0.4 +0.2
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +0.2 +0.0 -0.0 +0.7 +0.4
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +0.3 +0.1 +0.0 +0.7 +0.2
stress Spectinomycin 0.0125 mg/ml +0.1 +0.1 +0.4 -0.0 +0.8
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr +0.2 +0.0 +0.2 +0.4 +0.6
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